Yazar "Orcan, Pinar" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    Effect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey
    (Springer, 2020) Isikalan, Cidem; Orcan, Pinar; Akbas, Filiz; Namli, Sureyya; Kuru, Ibrahim Selcuk; Bulus, Serife
    Ajuga xylorrhiza Kit Tan is a rare endemic perennial species growing in the stony habitats in Southeastern Anatolia region, Turkey. An efficient and reproducible protocol has been developed for micropropagation of endangered Ajuga xylorrhiza. Shoots were cultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of 6-benzyl amino purine (BAP), thidiazuron (TDZ), and kinetin (Kin). The optimal shoot formation was with the medium containing 0.125 mg L-1 BAP (7.91 shoots per explant), and 0.25 mg L-1 Kin (4.9 shoots per explant) was recorded. The best result in terms of shoot length (3.43 to 3.63 cm) was obtained from the Kin treatments. Kin parameters were more effective on shoot length and multiple shoot production than BAP and TDZ. For rooting, regenerated shoots were transferred to 1/4 strength MS medium containing two different concentrations of indole acetic acid (IAA) and alpha-naphthalenetic acid (NAA) at 0 (control), 0.5, or 1.0 mg L-1.The best results in terms of the number of roots per shoot were obtained from the medium supplemented with 0.5 mg L-1 IAA (3.10 roots per explant). Rooted plantlets were transferred to soil for acclimization with 100% success rate.
  • [ X ]
    Öğe
    EVALUATION OF SALINITY TOLERANCE IN RICE (ORYZA SATIVA L.) USING WATER POTENTIAL, BIOMASS, MEMBRAN DAMAGE AND OSMOPROTECTIVE COMPOUND
    (Parlar Scientific Publications (P S P), 2019) Orcan, Pinar; Isikalan, Cigdem; Akbas, Filiz
    We investigated the physiological (biomass, water relative content-RWC) and biochemical responses (osmoprotective compound-prolin, membran damage-MDA) of rice (Oryza sativa L.) varieties against stress factor at different concentrations (100, 200 and 300 mM NaCI). 2 local populations (Karacadag, Hazro) and six rice varieties namely, Gala, Edirne, Sumnu, Negi, Tunca and Aromatik-1 were included in this study. As NaCI concentration increased, RWC decreased at different levels depending on the varieties, but the greatest decrease was detected in Gala (66%) and Karacadag (74%). However, Tunca (85%) and Aromatik-1 (86%) were selected as the least affected varieties even at high NaCI concentration (300 mM). After 10 days of applications, the increase in proline (pro) content varied according to the varieties of rice and duration of application. When the malondialdehyde (MDA) contents of the varieties were examined, it was determined that $umnu and Gala were highly affected even at 100 mM NaCI. Severe stress factor (300 mM NaCI) caused MDA increase in Tunca and Aromatik-1 but this increase were found higher in Karacadag, Neg4 and Gala. As a result, when all parameters are evaluated Gala and Karacadag was found a higher sensitivity while Tunca and Aromatik-1 had tolerant against salinity.