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    Detection of Theileria annulata and T. buffeli in Cattle by Multiplex PCR in Diyarbakir Area
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Deniz, Ahmet; Oncel, Taraneh; Icen, Hasan; Simsek, Aynur
    The aim of this study was to detect Theileria annulata and T. buffeli in healthy cattle by multiplex PCR method in Diyarbakir area of Turkey. For this purpose a total of 100 blood samples were collected within the tubes coated with EDTA from cattle in Diyarbakir area. At the same time, peripheral blood smears were also prepared from each animal for microscopic examination. Additionally, the cattle were also examined for tick infestation. Tick infestation was not observed on the examined cattle. In microscopic examination of the blood smears, 5 of the 100 cattle were detected as positive for Theileria spp. In molecular analyses of the blood samples, T. annulata was detected in 23 cattle by multiplex PCR. In one cattle, T. annulata + T. buffeli was also determined via multiplex PCR. Results indicated that, T. annulata is commonly observed in cattle in Diyarbakir.