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    Investigation of algerian crataegus monogyna jacq phenolic compounds (Using LC-ESI-MS/MS analysis, antioxidant activity, and enzyme inhibition) and their potential implications for food and nutraceutical applications
    (Multidisciplinary Digital Publishing Institute (MDPI), 2024) Goudjil, Sabrina; Boussekine, Samira; Goudjil, Sarra; Goudjil, Hanane; Yılmaz, Mustafa Abdullah; Ola, Mohammad Shamsul; Ali, Ahmad; Çakır, Oğuz
    Investigations into the phenolic constituents of the butanolic fraction of Crataegus monogyna were optimized using LC-ESI-MS/MS analysis, identifying and quantifying at least 23 fingerprint phytochemical compounds. The major phenolic compounds were epicatechin (99.916 ± 2.208 mg/g), isoquercetrin (53.31 ± 1.172 mg/g), chlorogenic acid (47.457 ± 1.010 mg/g), quinic acid (37.819 ± 1.406 mg/g), rutin (29.98 ± 0.740 mg/g), hesperidin (5.296 ± 0.177 mg/g, detected for the first time in the C. monogyna species), astragalin (1.774 ± 0.020 mg/g), and nicotiflorin (1.482 ± 0.016 mg/g). The antioxidant properties of the lyophilized butanolic fraction were evaluated using DPPH, GOR, ABTS, CUPRAC, and reducing power assays, all of which demonstrated that there was strong activity. Additionally, the neuroprotective effect was evaluated in vitro, showing a potent inhibitory effect on acetylcholinesterase (AChE) with an IC50 of 43.65 ± 2.10 µg/mL. The antidiabetic effect was investigated through α-amylase inhibition (IC50 = 91.19 ± 0.10 µg/mL), showing high inhibitory activity. In addition, the butanolic extract exhibited significant urease inhibition with an IC50 of 26.36 ± 0.05 µg/mL. These results suggest that Algerian C. monogyna has potential as a therapeutic agent for managing diabetes complications and as a natural source of AChE inhibitors, making it a promising subject for the treatment of urease-related conditions. Its high concentrations of natural antioxidants, such as epicatechin, isoquercetrin, chlorogenic acid, quinic acid, rutin, hesperidin, and astragalin, make it suitable for integration into medicine, pharmaceuticals, cosmetics, and the food sector.
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    Therapeutic potential of Hyoscyamus niger-derived compounds: Targeting ovarian cancer through antioxidant activity and EGFR tyrosine kinase inhibition
    (Elsevier B.V., 2024) Lekmine, Sabrina; Benslama, Ouided; Kadi, Kenza; Yılmaz, Mustafa Abdullah; Ali, Ahmad; Ola, Mohammad Shamsul; García, Antonio Ignacio Martín
    Ovarian cancer poses a significant challenge to women's health, necessitating innovative therapeutic approaches. This study investigates the therapeutic potential of compounds derived from Hyoscyamus niger, a medicinal plant with a history of traditional use, focusing on their antioxidant properties and their ability to inhibit epidermal growth factor-receptor (EGFR) tyrosine kinase a promising target in cancer therapy. LC-ESI-MS/MS analysis of H. niger extract revealed the presence of 21 phenolic compounds, with luteolin and p-coumaric acid notably exhibiting the highest concentrations. The extract displayed robust antioxidant activity across multiple assays, and its performance in the Reducing Power assay highlights its potential in reducing oxidative stress. Molecular docking results indicate that several phenolic compounds may serve as EGFR inhibitors, offering promising avenues for further investigation in cancer therapy. Notably, hyperoside emerged as a strong candidate due to its favorable binding energy and interactions within the EGFR active site. This study demonstrates the therapeutic promise of H. niger-derived compounds in targeting ovarian cancer through antioxidant activity and EGFR inhibition. Further research is warranted to validate their efficacy and safety, potentially opening up new avenues for ovarian cancer treatment.