Yazar "Namli, Suereyya" seçeneğine göre listele

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    Adventitious shoot development from leaf and stem explants of Amygdalus communis L. cv. Yaltinski
    (Southern Cross Publ, 2010) Isikalan, Cigdem; Akbas, Filiz; Namli, Suereyya; Basaran, Davut
    In the present research effect of plant growth regulators (PGRs) on callus formation and adventitious shoot from different explants of Amygdalus communis L.cv. Yaltinski was investigated. Callus was initiated from leaf and stem explants on MS medium supplemented with four different auxin (IAA, NAA, IBA and 2, 4-D) combined with BAP (1.0 mg l(-1)) under dark and light conditions. Among all the concentrations of auxin the best result from leaf explants was observed with the 1.0: 1.0 and 2.0:1.0 NAA plus BAP treatments (90%, 88% respectively) under light conditions. Only non-embryogenic callus on leaf explants obtained in the concentrations (1.0, 2.0, 4.0 mg l(-1)) of auxin. The obtained calli from leaf explants cultured on MS media containing different concentrations of BAP (1.0, 2.0, 4.0, 6.0, 8.0 mg l(-1)) for adventitious shoot development. Calli showed some proliferation after it was transferred to the media, but did not show any embryogenic response. In our research, the highest callus induction (80%) was obtained on medium containing 1.0: 1.0 2,4-D plus BAP, under the dark (7 days) conditions for stem explants. Moreover, at the same medium and under the same conditions embryogenic calli was obtained as well. Calli showed adventitious shoot development after it was transferred to medium containing 4.0 mg l(-1) BAP, but other concentrations of BAP (2.0, 6.0, 8.0 mg l(-1)) did not show any embryogenic response. The medium supplemented with 4.0: 1.0 IAA plus BAP provided root development directly from the yaltinski stem explants under the dark conditions (7 days).
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    Callus Induction and Plant Regeneration from Different Explants of Actinidia deliciosa
    (Springer, 2009) Akbas, Filiz; Isikalan, Cigdem; Namli, Suereyya
    In this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit (Actinidia deliciosa) using different organs of shoots developed under in vitro conditions. Effects of explants source and media (M-1, 1.0 mg l(-1) BA + 2.0 mg l(-1) 2,4-D-M-2, 1.0 mg l(-1) NAA + 2.0 mg l(-1) 2,4-D) on initiation of callus were examined in order to obtain callus for organogenesis. The best callus for plant regeneration was obtained from leaf explants on Murashige and Skoog's medium (MS) supplemented with M-2. Formation of callus from leaf of kiwifruit (A. deliciosa) was cultured in MS medium containing different concentration of N-6-benzylaminopurin (BA; 0.0, 1.0, 2.0, 4.0, 6.0, 8.0 mg l(-1)) for callus proliferation and plant regeneration. Although the first shoot formation was appeared in medium containing 6.0 and 8.0 mg l(-1) BA, the best shoots formation was obtained in medium with 4.0 mg l(-1) BA.
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    Cytogenetic Effects of Magnesium Sulphate on The Root Tip Cells of Pea (Pisum sativum L.cv.araka)
    (Asian Journal Of Chemistry, 2009) Ayaz, Emine; Namli, Suereyya
    The effects of magnesium sulphate on mitosis were investigated in Pisum sativum L.cv.araka. developed in vitro conditions. The roots of pea (Pisum sativum L.cv.araka) were treated with MgSO(4) concentrations of 1 -0.1 -0.01 mp/L for 3-6 and 12 h treatment periods. It was found that MuSO(4) has a marked mitodepressive action on mitosis. Magnesium sulphate significantly decreased the mitotic index at all concentrations and treatment periods. Mitotic abnormalities were increased and the mitotic index was decreased depending on the concentration and duration time of magnesium sulphate applied.
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    The effect of different plant hormones (PGRs) on multiple shoots of Hypericum retusum Aucher
    (Southern Cross Publ, 2010) Namli, Suereyya; Akbas, Filiz; Isikalan, Cigdem; Tilkat, Emine Ayaz; Basaran, Davut
    Use of Hypericum species have increased in the past few years due to the antidepressant and antiviral activities found in extracts of those plants. As a result of its potential as a pharmaceutical, a new system was developed for in vitro culture of this species. The goal of this investigation was to produce multiple shoot via in vitro techniques for Hypericum retusum Aucher. In vitro germination of the seeds was standardized on Murashige and Skoog (MS) hormone-free medium. Cultures were initiated from shoots inoculated onto MS medium supplemented individually with nine different concentrations of 6-Benzylaminopurine (BAP) and Kinetin (Kn). The highest number of shoots was obtained on medium supplemented with 0.5 mg l(-1) BAP (64.25 shoot/explant). Out of all the investigated concentrations of Kn, the best result was obtained on medium supplemented with 1.5 mg l(-1) Kn (27.87 shoot/explant). In addition, shoots were cultured separately on the media containing BAP (0.5 mgl(-1)) and Kn (1.5 mgl(-1)) combined with three different auxins (0.25 mgl(-1) IAA, IBA, NAA). In view of number and length of shoot the best result was obtained on the medium supplemented with 0.5 mg l(-1) BAP + 0.25 mg l(-1) IBA (54.12 shoot/explant, 3.36 length of shoot). In the presented study, the use of the BAP alone was the most efficient for shoot propagation. Rooted plantlets were acclimatized and successfully transferred to soil.
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    Effect of plant growth regulators on in vitro shoot multiplication of Amygdalus communis L. cv. Yaltsinki
    (Academic Journals, 2009) Akbas, Filiz; Isikalan, Cigdem; Namli, Suereyya; Ak, Bekir Erol
    An efficient protocol was established for in vitro shoot multiplication from apical shoot tips derived from mature trees of almond (Amygdalus communis L.) cultivars, Yaltsinki. Explants were cultured on Murashige and Skoog (1962) (MS) medium containing various concentrations of 6-benzyladenin (BA) and kinetin (kin) for shoot multiplication. Shoot multiplication was best achieved from explant on MS medium containing 30 gl(-1) sucrose, 7 gl(-1) agar and 1.0 mgl(-1) BA. This amount of BA (1.0 mgl(-1)) gave the best multiple shoot formation response with an average of 16.10 shoots per explant. In addition, shoots were cultured on the media containing 1.0 mgl(-1) BA and kin combined with three different auxins (0.25 and 0.5 mgl(-1) of IAA, IBA and NAA) separately. It was noted that 1.0 mgl(-1) BA and kin combinated with NAA had inhibitory effect on new shoot formation and no shoot formation was induced. However, explants cultivated on medium containing 1.0 mgl(-1) BA and 0.5 mgl(-1) IAA resulted in 11.25 shoots per explant. The effect of four different sucrose concentrations (20, 30, 40, 50 gl(-1)) on the multiplication of shoots was also investigated. The best shoot multiplication was obtained in MS media containing 30 gl(-1) sucrose with an average of 15.40 shoots per explant.
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    EFFECT OF UV-C ON PRODUCTION OF HYPERICIN IN Hypericum triquetrifolium Turra GROWN UNDER IN-VITRO CONDITIONS
    (Parlar Scientific Publications (P S P), 2009) Namli, Suereyya; Toker, Zuhal; Isikalan, Cigdem; Oezen, Hasan Cetin
    In this study, the effects of UV-C radiation on the increasing of hypericin in Hypericum triquetrifolium Turra were investigated by using in vitro techniques. The mature seeds of Hypericum triquetrifolium Turra were germinated in Murashige and Skoog (MS) medium without hormone under in vitro conditions, and the shoots obtained were successfully reproduced in MS medium supplemented with 0.25 mg L-1 BA (N-6-benzylaminopurine). The UV-C radiation was applied to Hypericum triquetrifolium Turra at different periods (15, 30, 45, 60 min). Although the hypericin content increased at 15-min UV-C radiation, it decreased after 60 min of application. Depending upon different parameters of UV-C radiation, some morphological alterations were observed in shoots.
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    In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil)
    (Academic Journals, 2008) Isikalan, Cigdem; Akbas, Filiz Adiyaman; Namli, Suereyya; Tilkat, Engin; Basaran, Davut
    An efficient in vitro propagation method was developed for almond (Amygdalus communis L. cv. Nonpareil). The effect of BA and kinetin (0.0, 0.5, 1.0, 2.0, 4.0 mgl(-1)) on the culture initiation of zygotic embryos isolated from mature seeds was investigated. A Murashige and Skoog (1962) (MS) medium containing 30 gl(-1) sucrose, 0.5 and 1.0 mgl(-1) N-6-benzylaminopurine (BA) and 7 gl(-1) agar resulted in a multiple shoot initiation at the rate of 11.0 +/- 1.32 and 14.7 +/- 2.12 shoot per explant, respectively, in 28 days of culture. The effects of a low concentration of BA (0.1, 0.5, 1.0 and 2.0 mgl(-1)) and different combinations of auxin + cytokinin were investigated for shoot proliferation. The best results for new shoot production were obtained from a MS culture medium which was supplemented with 1.0 mgl(-1) BA. The rooting was achieved in a MS medium supplemented with 8.0 mgl(-1) indole acetic acid (IAA). The in vitro raised plants were acclimatized in a growth room and successfully transplanted to the field. This method here in described will be useful for the rapid multiplication of almond (A. communis L. cv. Nonpareil) in commercial exploitation.
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    The karyotype analysis of Pistacia vera L. from Turkey
    (Taylor & Francis Ltd, 2009) Ayaz, Emine; Namli, Suereyya
    A detailed karyotype analysis was developed for Pistacia vera L. grown in Turkey. In vitro roots obtained from mature seeds were used as plant material. The chromosome number of P. vera L. was found to be 2n = 30 at c-metaphase of mitosis cell investigated for all of the materials. Centromere type of all chromosomes were determinated as median, submedian, subtelocentric, telocentric and total lengths of chromosome pairs were found between 35.4 and 5.97 m. An idiogram was constructed from the average chromosome length, arm ratio and centomere type for each of the chromosome pairs.