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    Öğe
    A comparative evaluation of the effects of bevacizumab and 5-fluorouracil on wound healing in rat model
    (Drunpp-Sarajevo, 2012) Gunay, Ahmet; Atilgan, Serhat; Yesilova, Yavuz; Yaman, Ferhan; Ozgoz, Mehmet; Lacin, Nihat
    Purpose: Our aim is to evaluate the use of combined bevacizumab with 5-Fluorouracil (5-FU) on post-operative scarring survival following experimental skin incision surgery in comparison to the agents alone. Material and methods: Skin incision surgery was performed on 28 female Spraque-Dawley rats. The rats were allocated to one of four treatments: 5-FU combined with Bevacizumab, 5FU alone, bevacizumab alone and phosphate buffered saline (PBS). A single subcutaneous injection was administered immediate postoperatively. Histological staining determined the presence of cutaneous fibrosis and mRNA expression of collagen I and fibronectin in the tissue was quantified. Results: Relative quantity of Colla1 mRNA transcript 5 fold increased in Group 1 (PBS) in comparison to control group. However, Colla1 level in Group 2 (5-FU) and 3 (bevacizumab) is decreased. The lowest level of Colla1 was detected in Group 4 (combination of 5-FU and bevacizumab). On the other hand, relative quantity of Fn1 is slightly elevated in Group 1 (PBS) and decreased in Group 2 (5-FU) and group 3 (bevacizumab) in comparison to control group. The lowest level of Fn 1 was detected in Group 4 (combination of 5-FU and bevacizumab). Conclusions: Bevacizumab in combination with 5-FU resulted in a greater anti-fibrotic effect compared to monotherapy with 5FU or bevacizumab alone, as evidenced by the attenuation in fibronectin and mature collagen I expression and deposition. (P<0.05) The results provide compelling evidence that combined bevacizumab and 5-FU offers superior anti-fibrotic effect over monotherapy. A synergistic effect is suggested to be present.
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    Öğe
    Evaluation of the Regeneration Effects of Systemically Applied Methylprednisolone and Vitamin B12 on Mental Nerve Crush Injury
    (Turkish Neurosurgical Society, 2024) Kara, Cigdem; Lacin, Nihat; Adak, Berat Metin; Kara, Alper; Tunik, Selcuk; Uysal, Ersin; Soylu, Fahri Emrah
    AIM: To evaluate the effects of methylprednisolone and vitamin B12 injection on the regeneration of the nerves after a mental nerve crush injury. MATERIAL and METHODS: A total of 40 albino Wistar rats have been randomly divided into four groups: Ynt: ÖNEMLİ: Turkish Neurosurgery_JTN 43470_proof reading group 1 (n=10): crush-like nerve damage was created by pinching the left mental nerve for 60 s with an aneurysm clip and intraperitoneally administered 1 ml of saline for 14 days; group 2 (n=10): the left mental nerve was pinched for 60 s with an aneurysm clip and intraperitoneally administered 2 mg/kg of methylprednisolone for 14 days; group 3, experimental group (n=10): the left mental nerve was pinched for 60 s with an aneurysm clip and intraperitoneally administered 2 mg/kg of vitamin B12 for 14 days; and group 4, experimental group (n=10): the left mental nerve was pinched for 60 s and intraperitoneally administered 2 mg/kg of methylprednisolone and 2 mg/kg of vitamin B12 for 14 days. All rats were sacrificed on the 28th postoperative day, and histopathological evaluation was performed. RESULTS: Nerve damage was higher in the control group than in other groups (p<0.05). When Neural Cell Adhesion Molecule (NCAM) expression levels were compared, no major differences were observed between the methylprednisolone and control groups (p>0.05). The B12 and B12+methylprednisolone groups reached significantly higher NCAM expression levels compared to the control and methylprednisolone groups. When the myelin basic protein (MBP) expression levels were compared (p<0.05), the MBP expression was significantly higher in all experimental groups than in the control group (p<0.05). CONCLUSION: Systemic vitamin B12 and methylprednisolone administration effectively supported remyelination in the crushed mental nerve by increasing Schwann cell proliferation and differentiation. © (2024), (Turkish Neurosurgical Society). All rights reserved.