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    Effects of presence or absence of a dominant follicle estimated by a single ultrasound examination at the time of follicular aspiration on superovulatory responses and embryo production in lactating simmental cows
    (Kafkas Üniversitesi Veteriner Fakültesi, 2019) Cirit, Ümüt; Özmen, Mehmet Ferit; Köse, Mehmet; Küçükaslan, İbrahim; Çınar, Elif Merve; Kutsal, Hüseyin Gökhan
    The aim of the study was to evaluate effects of presence or absence of a dominant follicle (DF) estimated by a single ultrasound examination atthe time of follicular aspiration (FA) on superstimulatory and superovulatory responses and embryo production in lactating Simmental cows. Atrandom stages of the estrous cycle, the ovaries of cows (n=42) were examined by transrectal ultrasonography (US) and all follicles ≥3 mm werecounted. Donors with <10 follicles 3-8 mm in diameter were considered to have a dominant follicle (group DF+; n=30), while donors ≥10 smallfollicles 3-8 mm were classified as having no dominant follicle (group DF–; n=12). Just after US examination, all cows were subjected to ultrasoundguidedtransvaginal aspiration of all follicles ≥5 mm and a progesterone-releasing device was placed in the vagina. Thirty-six h after FA, all cows weresuperstimulated with FSH, which was given as twice-daily injections over 6 days. Cows were pre-treated with a single dose of 400 IU of eCG 24 h beforethe start of FSH treatments. It was concluded from this study that the presence of a DF estimated by a single ultrasound examination at the time of FAeffects negatively the superstimulatory and superovulatory responses, fertilization rate and embryo quality (P<0.05) but not the number of embryoscollected. It was also concluded that estimation of a dominant follicle by a single ultrasound examination at the time of follicular aspiration based onthe number of small follicles may be used the selection of potential donor cows and can significantly contribute to improvements in superstimulatoryand superovulatory responses and embryo quality.