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    Effect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey
    (Springer, 2020) Isikalan, Cidem; Orcan, Pinar; Akbas, Filiz; Namli, Sureyya; Kuru, Ibrahim Selcuk; Bulus, Serife
    Ajuga xylorrhiza Kit Tan is a rare endemic perennial species growing in the stony habitats in Southeastern Anatolia region, Turkey. An efficient and reproducible protocol has been developed for micropropagation of endangered Ajuga xylorrhiza. Shoots were cultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of 6-benzyl amino purine (BAP), thidiazuron (TDZ), and kinetin (Kin). The optimal shoot formation was with the medium containing 0.125 mg L-1 BAP (7.91 shoots per explant), and 0.25 mg L-1 Kin (4.9 shoots per explant) was recorded. The best result in terms of shoot length (3.43 to 3.63 cm) was obtained from the Kin treatments. Kin parameters were more effective on shoot length and multiple shoot production than BAP and TDZ. For rooting, regenerated shoots were transferred to 1/4 strength MS medium containing two different concentrations of indole acetic acid (IAA) and alpha-naphthalenetic acid (NAA) at 0 (control), 0.5, or 1.0 mg L-1.The best results in terms of the number of roots per shoot were obtained from the medium supplemented with 0.5 mg L-1 IAA (3.10 roots per explant). Rooted plantlets were transferred to soil for acclimization with 100% success rate.
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    Öğe
    THE EFFECT OF UV-B ON FATTY ACID CONTENT AND RADICAL SCAVENGING ACTIVITY OF METHANOLIC EXTRACTS FROM Hypericum retusum Aucher GROWN UNDER IN VITRO CONDITIONS
    (Parlar Scientific Publications (P S P), 2013) Isikalan, Cigdem; Karakus, Pinar; Kuru, Ibrahim Selcuk; Celik, Kadir Serdar
    The study was aimed to evaluate the effects of UV-B radiation on fatty acid content and antioxidant activity of a methanolic extract of Hypericum retusum Aucher plantlets grown under in vitro conditions. Therefore, the plantlets were exposed to UV-B radiation for different durations (Ti: 15, T-2: 30, T-3: 45, T-4: 60 min) The UV-B application led to increase in linolenic (42.84%), oleic (6.92%) and linoleic acid (28.48%) in H. retusum. The methanol extracts of plantlets grown in vitro conditions showed the strongest free radical scavenging activities at concentrations of 50, 100, and 300 mu g/mL(-1). It has been found that UV-B radiation and in vitro growth conditions have a significant effect on the accumulation of fatty acids. However, the free radical scavenging ability of H. retusum extract was decreased after UV-B application.