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Öğe The antioxidant effect of Michauxia campanuloides on rat ovaries(Hellenic Veterinary Medical Society, 2021) Kurt, S.; Koca, R. H.; Hürkul, M. M.; Şeker, U.; Köroğlu, A.This study investigated how Michauxia campanuloides affects the Proliferating Cell Nuclear Antigen (PCNA) expression in granulosa cells, the ovarian histomorphology and serum total antioxidant capacity (TAC) in rats. Rats were divided into control (C), treatment 1 (T 1) and treatment 2 (T 2) groups. The rats in the T 1 and T 2 groups received aqueous extract of M. campanuloides at doses of 20 mg/kg/day and 40 mg/kg/day orally for 21 days, respectively. Serum TAC levels, follicles counts including primordial, primary, preantral, antral and atretic follicles, and PCNA expression in granulosa cells were evaluated. Numbers of preantral follicles increased in T 1 and T 2 groups compared to C group (P < 0.05). TAC and numbers of preantral and antral follicles increased in T 2 group compared to T 1 and C groups (P < 0.05). PCNA expression in granulosa cells was increased in T 2 group compared to T 1 and C groups (P <0.01). In conclusion, treatment with M. campanuloides had positive effects on antioxidant activity, follicular dynamics, and PCNA expression of granulosa cell in rats.Öğe Investigation of the relationship between some metabolic and antioxidants parameters and pregnancy success at the first postpartum artificial insemination in primiparous cows(Wiley, 2021) Yazliki, M.O.; Kurt, S.; Terzi, O.S.; Kaya, U.Investigation of the relationship between some metabolic and antioxidants parameters and pregnancy success at the first postpartum artificial insemination in primiparous cowsÖğe Oxidative stress in cows according to calving season: passive calf immunity and its relationship with colostrum quality(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2021) Colakoglu, H. E.; Yazlik, M. O.; Colakoglu, E. C.; Kaya, U.; Bayramoglu, R.; Kurt, S.; Vural, R.This study details the relationship between maternal plasma oxidant-antioxidant enzymes with colostrum quality, serum gamma glutamyl transferase (GGT), immunoglobulin G (IgG) and IgM concentrations of calves in the different calving seasons. Holstein breed cows between two and eight lactations and their calves were enrolled in the study. Holstein cows calving in winter (n=45) and their calves (n=45) were assigned to the winter group, while cows calving in summer (n=45) and their calves (n=45) were assigned to the summer group. Samples for malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were collected on day -21 +/- 3 before expected calving and also on calving day (Day 0). IgG and the specific gravity of the colostrun were determined after calving. Serum GGT and IgG and IgM were measured before the feeding, with colostrum, of calves (0 hours) and also in the 24th hour following the feeding of colostrum. Plasma MDA levels at -21 +/- 3 and 0 days in the summer cows were determined to be higher. GSH-Px activity was higher in the winter cows. IgG levels and the specific gravity of the colostrum were also higher in the winter cows. Calf IgG levels at the 24th hour of life were higher in the winter cows. In the winter group, IgM levels at 0 and 24 hours were also higher. While MDA was negatively correlated with IgG, IgM, GGT, IgG and the specific gravity of colostrum, GSH-Px activity had a positive correlation with IgG. IgM, GGT, IgG and the specific gravity of colostrum. The observed differences in plasma MDA, GSH-Px, calf serum IgG and IgM levels, and colostrum quality between both groups suggest a possible seasonal effect. The relationship between maternal oxidant-antioxidant enzymes, colostrum quality, and passive calf immunity revealed that these enzymes could be used as indicators in the evaluation of calf health and colostrum quality.