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Öğe Acupuncture stimulation causes earlier increase in the size and blood flow of corpus luteum and progesterone levels in dairy cows(Wiley-Blackwell, 2015) Kucukaslan, I.; Aslan, S.; Ay, S. S.; Kaya, D.; Findik, M.; Kacar, C.; Wollgarten, B.[Abstract Not Available]Öğe Beneficial effects of Beta-carotene injections prior to treatment with PGF2? on the fertility of postpartum dairy cows(Ecole Nationale Veterinaire Toulouse, 2012) Ay, S. S.; Kaya, D.; Kucukaslan, I.; Agaoglu, A. R.; Emre, B.; Handler, J.; Findik, M.This study examined the effects of Beta-carotene (beta C) injections before estrus synchronization with PGF(2)alpha on fertility parameters in cows. A total of 124 postpartum (pp) cows were used. In GI (n=25), beta C (Carofertin (R), 0.4 mg/kgBW, i.m.) was injected on d 15 and 45 pp. In GII (n=25), beta C was administered on d 15 pp. In GIII (n=25), beta C was administered on d 45 pp. In GIV (n=25), beta C was injected on d 35 and 45 pp. GV (n=24) was set aside as a control group. All animals received PGF(2)alpha (Iliren (R), 0.0015 mg/kgBW, i.m.) on d 50 and 61 pp. Blood samples were collected on d 15,48 and three days after each beta C injection. The overall pregnancy rate was determined to be higher in GI (100%) and GIV (88.0%) than GV (70.8%) (P<0.01). The number of services per conception was the lowest in GI (1.4) and GII (1.4), while days of first service were lower in GIII (90.3 d) and GIV (90.8 d) (P<0.05). The insemination index was found to be lowest in GI (1.4). These results suggest that two administrations of beta C in the early and late pp period before administering PGF(2)alpha can improve fertility. It was concluded that when serum beta C concentrations remain high for a long time (30d) as a results of beta C injections, fertility can be improved.Öğe Characterization of Oestrous Induction Response, Oestrous Duration, Fecundity and Fertility in Awassi Ewes During the Non-breeding Season Utilizing both CIDR and Intravaginal Sponge Treatments(Wiley, 2010) Ozyurtlu, N.; Kucukaslan, I.; Cetin, Y.Contents The aim of this study was to investigate characterization of oestrous response, onset of induced oestrus, oestrous duration, fecundity and fertility in Awassi ewes treatment with intravaginal sponges and Controlled Intravaginal Drug Release (CIDR) devices in combination with pregnant mare serum gonadotropin (PMSG) under local environmental conditions during the non-breeding season. A total of 62 ewes were divided into three groups. Group CIDR (n = 20) was treated with CIDR devices for 12 days and 400 IU PMSG was injected upon removal of the CIDR. For ewes in Group Sponge (SP) (n = 24), 30 mg fluorogestone acetate was administered to the sheep for 12 days and 400 IU PMSG was injected upon withdrawal of the sponge. Group Control (CON) (n = 18) served as a control group and received no treatment. Adult, intact and sexually experienced Awassi rams were introduced to all groups at the time when the intravaginal devices were removed. There were no significant differences in terms of oestrous response (CIDR: 90%, SP: 87.5%), time to onset of oestrus and duration of induced oestrus between the CIDR and SP groups. The oestrous response of treatment groups was significantly greater (p < 0.05) than in the control ewes. There were no significant differences in pregnancy (CIDR: 70%, SP: 70.8%), lambing (CIDR: 85%, SP: 79.2%) and fecundity rates between ewes treated with CIDR and those treated with sponges. However, pregnancy and lambing rates were significantly (p < 0.05) higher in ewes treated with CIDR or sponges when compared with those in the control group. It was concluded that it is possible to induce fertile oestrus, successful pregnancy and lambing with the treatment of either CIDR or intravaginal sponge in combination with PMSG in Awassi ewes during the non-breeding season.Öğe Clinical and endocrinological effects of two different treatments for the induction of abortion in bitches(Wiley-Blackwell, 2011) Kaya, D.; Kucukaslan, I.; Agaoglu, A.; Ay, S.; Schafer-Somi, S.; Emre, B.; Bal, Y.[Abstract Not Available]Öğe The effect of acupuncture application during diestrus periods on the size and blood flow of corpus luteum and hormonal parameters in dairy cows(Wiley-Blackwell, 2014) Kucukaslan, I.; Ay, S. S.; Kaya, D.; Findik, M.; Aslan, S.[Abstract Not Available]Öğe The effectiveness of combined preventive treatment with Ceftiofur, Oxytocin and PGF2? on fertility parameters in cows(Ecole Nationale Veterinaire Toulouse, 2012) Kaya, D.; Ay, S. S.; Kucukaslan, I.; Beceriklisoy, H. B.; Agaoglu, A. R.; Findik, M.; Ozyurtlu, N.The present study is aimed at evaluating the effectiveness of combined preventive treatment with Ceftiofur Hydrochloride (CHR), Oxytocin (OXT) and PGF(2 alpha) on fertility parameters and puerperal disorder rates in postpartum (pp) dairy cows. A total of 191 Holstein-Friesian cows were divided into four groups. The cows in group I (GI; n=37) and II (GII; n=69) were treated on a daily basis with CHR and OXT for five and three days pp, respectively. Group III (GIII; n=52) was designed as the PGF(2 alpha) group, and group IV (GIV; n=33) served as a control group. The animals in GI, GII, and Gill were given a PGF(2 alpha) analogue on days 15 and 26 pp. Each group was further divided into subgroups of normal parturition (np) and dystocia (d) when the study results were evaluated. It was found that the first service pregnancy rates for the subgroup of dystocia were significantly higher (P<0.05 and P<0.01) in subgroup GII (76.9%) than in GI (30.0%) and GIV (37.5%). Furthermore, the median time to first service and the median time to pregnancy were longer for cows in subgroup GIVd as compared with subgroups GId, GIId, and GIIId (P<0.001; P<0.01). Group II was found to have the lowest endometritis rates after treatment of subgroups-d and -np. No statistically significant difference was found between the groups with regard to cystic ovary rates after treatment (P>0.05). As the treatment with CHR-OXT combined with PGF(2 alpha) on days 15 and 26 was associated to improved uterine involution patterns and reproductive performance, our results suggest that administration of this preventive treatment would be useful, especially for cases of dystocia.Öğe The effects of aglepristone alone and in combination with cloprostenol on hormonal values during termination of mid-term pregnancy in bitches(Elsevier Science Bv, 2014) Kaya, D.; Kucukaslan, I.; Agaoglu, A. R.; Ay, S. S.; Schaefer-Somi, S.; Emre, B.; Bal, Y.This study was designed to assess endocrine changes associated with termination of midterm pregnancy after use of two different protocols. For this purpose we compared the effects of aglepristone (AGL) alone and in combination with cloprostenol (CLO) on serum concentrations of progesterone (P-4), estradiol (E-2) and relaxin (RLN) measured at shortterm intervals during the abortion period in bitches. Fourteen pregnant bitches between day 25 and 32 of gestation were used in the study. In the AGL group (n = 7), aglepristone was administered solely (10 mg/kg body weight (BW), subcutaneously, once daily on two consecutive days) whereas in the AGL-CLO group (n = 7), aglepristone (dosage as in AGL group) and cloprostenol (1 mu g/kg BW, subcutaneously, same with aglepristone) were combined. All pregnancies were successfully terminated 5.2 1.6 days after initiation of treatments, which was significant in both groups (P> 0.05). At the time of the start of abortion (SA) and the end of abortion (EA), the mean P4 concentrations were 26.6 7.3 and 12.0 6.4 ng/ml in AGL group, and 2.7 0.7 and 0.9 0.1 ng/ml, in AGL-CLO group, respectively (P< 0.01). Serum E-2 concentrations were significantly higher (P< 0.05) in AGL group at 42, 48, 54 hand SA after initiation of treatment. In the AGL-CLO group, serum RLN concentrations did not significantly change from the initiation of treatment to EA (P> 0.05). However, markedly higher RLN concentrations (P< 0.05) were observed in the AGL group at 48 h (1.5 0.7 ng/ml) and at SA (1.6 0.5 ng/ml). The results of the present study indicate that changes in the hormonal concentrations affect the mechanism of abortion in different ways. Further in depth studies investigating changes in the expression of hormone receptors inside the ovary, endometrium and placenta might be helpful to our understanding of the endocrinological differences observed in this study. (C) 2014 Elsevier B.V. All rights reserved.Öğe Endometrial echotexture parameters in Turkish Saanen Goats (Akkeci) during oestrus and early pregnancy(Elsevier Science Bv, 2014) Cengiz, M.; Kanca, H.; Salar, S.; Bastan, A.; Kucukaslan, I.; Alkan, H.; Karakas, K.This experiment was conducted to evaluate endometrial echotexture in oestrus and early pregnancy and its association with ovarian hormones and foetal count in goats. Akkeci goats (Saanen x Kills crossbreed, n = 40) were randomly divided into two groups. Ten does (NAT) were mated on natural oestrus and 30 does (SYN) were subjected to synchronisation-prior to mating. The uterus was scanned on the days of sponge insertion (d - 14), sponge removal (d - 2) and mating (d 0) as well as 17 (d 17) and 30 (d 30) days after mating. Mean gray level (MGL), homogeneity (HOM) and contrast (CON) values were calculated. Blood samples were collected on days ultrasonography was performed. Data were analyzed by Chi-square, ANOVA, regression tests. HOM value reached the highest level on the mating day and then continuously decreased (P<0.0001). Overall, HOM values were greater for SYN does than for NAT does after mating. CON values were virtually stable during the experimental period. MGL value fluctuated during the breeding period (P<0.03) at a similar fashion in NAT and SYN does. Foetal count was not correlated with plasma hormones and echotexture parameters. Plasma progesterone concentration was correlated with echotexture parameters (r=-0.28 for HOM; r=0.29 for CON; r=0.25 for MGL; P<0.05 for all) during post-mating. In conclusion, echotexture parameters changed during the breeding period, in association with plasma progesterone concentration. Future studies should test if the echotextural changes during embryonic fixation days can be used as a marker for early detection of pregnancy in does. (C) 2014 Elsevier B.V. All rights reserved.Öğe Evaluation of endometrial echotexture and cervical cytology in cows during and after treatment of endometritis(Georg Thieme Verlag Kg, 2014) Kucukaslan, I.; Kaya, D.; Emre, B.; Bollwein, H.; Ozyurtlu, N.; Mulazimoglu, S. B.; Aslan, S.Objective: The aim of this study was to evaluate changes in the endometrium by using echotexture parameters during and after treatment of endometritis with intrauterine administration of an intrauterine antiseptic solution (Lotagen (R), 3% metacresolsulphonic acid and formaldehyde) in cows which became pregnant after treatment. Material and methods: According to the severity of endometritis 21 cows were divided into three groups: El (slight, n = 7), E2 (moderate, n = 8), E3 (severe, n = 6). The control group (C, n = 11) consisted of cows without endometritis that did not receive an intrauterine medication. A software (Bs200 Pro (R)) was used to evaluate echotexture parameters Contrast (CON), Gradient (GR), Homogeneity (HUM), Mean Gray Level (MGL) of images taken during the examinations at hours (h) 0, 1 and 6 and days (d) 2, 3, 5 and 10. Results: At 0 h, GR was significantly lower in group E2 than in groups El and C (p < 0.05). There was an increase in GR values between 0 h and 10 d in group E2 and E3, but a decrease during the same time interval in group C (p < 0.05). In contrast, CON values of group E2 were lower (p < 0.05) at 0 h compared to other timepoints of examination and lower than in group C. HOM values were lower (p < 0.05) in groups El, E2 and E3 than in group Con d 5 and d 10. HUM values were higher at 1 h compared to 6 h, d 2 and d 10 in group E3 (p < 0.05). By contrast to GR values, HUM values were higher in group C at 6 h and d 10 than they were in group E3. MGL values of group E2 were higher (p < 0.05) than in group C until d 10 and higher (p < 0.05) in group E3 than in group C at 6 h after treatment. In group E2 an increase of MGL values until d 2 was followed by a decrease (p < 0.05). Conclusion: Echotexture parameters determined by the evaluation of sonographic B-mode images reflect changes in the endometrium and could be used for the evaluation of the recovery period after treatment of endometritis.Öğe Expression profile of interferon tau-stimulated genes in ovine peripheral blood leukocytes during embryonic death(Elsevier Science Inc, 2016) Kose, M.; Kaya, M. S.; Aydilek, N.; Kucukaslan, I.; Bayril, T.; Bademkiran, S.; Kiyma, Z.Early and efficient detection of embryonic death (ED) has a valuable impact as important as early pregnancy diagnosis in ruminants. Among early pregnancy diagnosis methods, detection of the expression of interferon tau stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) is well documented in cows and ewes. Therefore, we hypothesized that the expression profile of ISGs in PBLs might also be useful for detecting ED in these animals. For this purpose, pregnant ewes were used as an experimental model. Pregnancy was detected on Day 18 after mating by transrectal ultrasonography. Pregnant ewes were divided into a control group (sham injection on Day 18, n = 10) and ED group (treated with 75 mu g synthetic PGF(2 alpha) on Day 18, n = 12). PBLs and plasma were collected on Days 0 (mating day), 15, 18, 19, 20, 21, 23, and 25 by jugular venipuncture. Total RNA was isolated from PBLs. ISGs expression levels were determined by real-time polymerase chain reaction in triplicate. Electrochemiluminescence immunoassay was used to measure progesterone (P-4) levels in plasma. In the ED group, the P-4 level declined to less than 1 ng/mL on Day 19 and remained at a low level until the end of the study. Compared with that on Day 0, receptor transporter protein 4 (RTP4) and ISG15 expression was upregulated on Day 15 and remained high until Day 21 in both groups, and RTP4 and ISG15 mRNA levels were attenuated on Days 23 and 25 only in the ED group (P < 0.001). Myxovirus resistance 1 expression was upregulated on Day 15 and remained high until Day 23 in both groups, but was attenuated on Day 25 in the ED group (P < 0.05). The B2-microglobulin mRNA level did not change significantly during the study in either group. These results indicate that the decline in P-4 concentration was an immediate response to PGF(2 alpha) and that the embryo may have survived longer than the CL on the basis of the extended period of ISGs expression. This suggests that the absence of P-4 could be the reason for ED rather than a direct effect of PGF(2 alpha). In conclusion, the expression of ISGs, including ISG15, RTP4, and myxovirus resistance 1, but not B2-microglobulin, in PBLs may serve as a marker of ED. (C) 2016 Elsevier Inc. All rights reserved.Öğe Expression profile of receptor transporter protein 4 (Rtp4) mRNA in ovine peripheral blood leucocytes (PBLs) during early embryonic death (EED)(Wiley-Blackwell, 2014) Kose, M.; Kaya, M. S.; Aydilek, N.; Kucukaslan, I.; Bayril, T.; Bademkiran, S.; Kiyma, Z.[Abstract Not Available]Öğe GnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterus(Elsevier Science Inc, 2015) Schaefer-Somi, S.; Kowalewski, M. P.; Kanca, H.; Bozkurt, M. P.; Gram, A.; Sabitzer, S.; Kucukaslan, I.In reproductive tissues, GnRH participates in the regulation of cell growth and proliferation by direct binding to the GnRH-R, which is essential for embryo implantation. However, there is no study on the expression and cellular localization of GnRH and GnRH-R in the canine uterus and placenta. Therefore, bitches were ovariohysterectomized 10 to 12 days after mating (vaginal cytology and progesterone measurement), the uteri were flushed, and if embryos were detectable, bitches were allocated to the embryo positive group (E-pos.; preimplantation, n = 5). Other bitches were operated at later stages and, dependent on the gestational age, either allotted to the post-implantation group (Day 18-25 after mating, n = 9), or the mid-gestation group (Day 30-40 after mating, n = 3). Dogs negative in embryo flushing served as controls (E-neg.; controls, n = 5). Samples of the entire uterine wall were taken from the middle of the horn in E-neg. and E-pos. groups, and from placental and interplacental uterine sites in post-implantation and mid-gestation groups. GnRH-R expression was localized at the mRNA and protein levels by immunohistochemistry and in situ hybridization. The expression of GnRH and GnRH-R mRNA was assessed by semi-quantitative polymerase chain reaction. Additionally, both GnRH and GnRH-R mRNA were expressed in all tissues examined until mid-gestation. Relative expression of GnRH was higher than that of GnRH-R (P < 0.05). During the post-implantation stage, GnRH-R expression was significantly higher in uteroplacental than in interplacental tissues. In the uterus, GnRH-R stained strongly in the surface and glandular epithelial cells, and seemed to be weaker in myometrium and stroma. Placental signals were predominantly localized in fetal trophoblast cells and to a lesser extent in maternal decidual cells. These findings suggest a local regulatory function of GnRH during early canine pregnancy. (C) 2015 Elsevier Inc. All rights reserved.Öğe Is Apoptosis a Regulatory Mechanism During Early Canine Pregnancy?(Wiley-Blackwell, 2012) Schaefer-Somi, S.; Sabitzer, S.; Klein, D.; Tomaszewski, C.; Kanca, H.; Beceriklisoy, H. B.; Kucukaslan, I.Contents Fas is a membrane-bound protein which upon activation causes programmed cell death. Fas ligand (FasL) binds Fas on target cells. Both these factors are known to regulate apoptosis at implantation in different species and thus might be involved in the regulation of implantation in dogs. The aim of the study was to assess the expression of Fas and FasL in canine uterine tissue throughout pregnancy as well as in pre-implantation embryos using RT-PCR and RT-qPCR. Uterine tissues was collected from of 21 healthy pregnant bitches (group I: days 1012, n=5; group II: days 1825, n=6; group III: days 2845, n=6) and from 4 non-pregnant bitches (controls: days 1012). Pregnancy stage was determined by days after mating, that is, 23days after ovulation as determined by vaginal cytology and progesterone measurement. After ovariohysterectomy, uteri from group I bitches were flushed with PBS and the embryos washed and stored frozen at -80 degrees. Tissues from the other groups were taken from the implantation and placentation sites, respectively, covered with Tissue Tek (R) and frozen at -80 degrees. Extraction of RNA was performed with Trizol Reagent and RT-qPCR using SYBR green probes. In pre-implantation embryos, only FasL but not Fas could be detected. In all tissues from pregnant and non-pregnant bitches, both parameters were detectable. Before implantation (group I) expression of FasL resembled that of non-pregnant bitches in early dioestrus and decreased significantly during implantation and thereafter (p<0.05). Expression of Fas did not change significantly until day 45. The relative expression of Fas exceeded that of FasL at each stage investigated, which is comparable to observations of other species; however, high standard deviations indicate high individual differences. These preliminary results point towards a regulatory function of the Fas/FasL system during early canine pregnancy.Öğe Use of Echo-texture Parameters for the Determination of Different Degree Endometritis in Cows and Evaluation of the Recovery Period after Treatment(Wiley-Blackwell Publishing, Inc, 2009) Kucukaslan, I.; Kaya, D.; Emre, B.; Ozyurtlu, N.; Aslan, S.[Abstract Not Available]Öğe Vascular Endothelial (VEGF) and Epidermal Growth Factor (EGF) as well as Platelet Activating Factor (PAF) and receptors are expressed in the early pregnant canine uterus(Wiley-Blackwell, 2012) Schaefer-Somi, S.; Sabitzer, S.; Klein, D.; Reinbacher, E.; Kanca, H.; Beceriklisoy, H. B.; Kucukaslan, I.[Abstract Not Available]
