Yazar "Koylu, Mehmet Zafer" seçeneğine göre listele

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    Determination of Proton Relaxivities of Mn(II), Cu(II) and Cr(III) added to Solutions of Serum Proteins
    (Mdpi, 2009) Koylu, Mehmet Zafer; Asubay, Sezai; Yilmaz, Ali
    Relaxometric studies are still of scientific interest due to their use in medicine and biology. In this study, proton T-1 and T-2 relaxivities of Mn(II), Cu(II) and Cr(III) in water were determined in the presence and absence of various proteins (albumin, alpha-globulin, gamma-globulin, lysozyme, fibrinogen). The 1/T-1 and 1/T-2 in all solutions are linearly proportional to the concentration of the paramagnetic ions. Mn(II) has the great influence to alter relaxations in all protein solutions, while Cu(II) and Cr(III) have a poor influence on the relaxations. In addition, Mn(II) and Cu(II) are bound to each protein, but Cr(III) is not bound to any protein.
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    Elimination of Radiation Damping Effects from the NMR Relaxation Curves of H2O/D2O Mixtures Containing Protein and Ions
    (Physical Soc Republic China, 2013) Zengin, Bilgin; Koylu, Mehmet Zafer; Korunur, Sibel; Yilmaz, Ali
    In this work the T-1 and T-2 curves of H2O/D2O mixtures were obtained for several fractions of H2O and also for different amounts of albumin and manganese. The experiments were carried out with a 400 MHz proton NMR spectrometer. The T-1 and the T-2 curves were determined by the inversion recovery (IR) and Carr-Purcell-Meiboom-Gill (CPMG) sequences, respectively. The relaxation times in residual water and in a mixture with 0.05 ml H2O were found to be reliable either in the presence or in the absence of albumin. The mixtures containing 0.1 ml or higher H2O suffer from radiation damping (RD). RD was removed gradually by addition of albumin or manganese. RD in the protein solution with 0.1 ml H2O was removed by adding 1 mu g of manganese to one ml of solution. The data suggest that the relaxation times in H2O/D2O mixtures are measurable by conventional methods upon the addition of appropriate amounts of manganese and albumin.
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    Öğe
    Separation of Line Widths of HOD Peaks of Healthy and Diseased Blood and Urine Groups Using 400 MHz NMR
    (Springer, 2025) Sakar, Mehmet Nafi; Yilmaz, Utku Nezih; Koylu, Mehmet Zafer
    Line width studies of high field nuclear magnetic resonance (NMR) peaks have been seen more frequently in the recent literature. This study aims to compare the NMR line widths of HOD peaks of healthy and diseased blood, and densely bloody cysts taken from the jaw. Comparisons of HOD peak line widths in healthy and diseased urine were also made. For this purpose, the blood, serum, and urine of 29 cancer patients, 17 diabetic patients, 28 healthy volunteers, and 20 intensely bloody samples from jaw cysts were collected. Mixtures were prepared by adding the 0.02-mL sample to 0.98 mL D2O. Single-pulse proton NMR measurements were performed at 400 MHz, and line widths were obtained from the half-height of the blood HOD peak. Statistical evaluations show that the line width of cancerous blood is different from normal blood (P = 0.032), while the line width of diabetic blood is not different (p = 0.072), whereas other groups and bloody jaw cysts are completely different (p < 0.05). Similar results were found for urine groups. Line widths show a moderate correlation with each of the albumin and total protein groups (R around 0,56). Present data suggest that a comparison of healthy and diseased body fluids can be made by line width measurements of the HOD peak in the high NMR field. Data also suggest that the relaxivities of albumin (Ser-Alb), total protein (Ser-TP) are potential diagnostic indicators for cancerous blood.