Yazar "Kochan, Akin" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • [ X ]
    Öğe
    Clinical, Haematological and Biochemical Findings in Sheep Naturally Infected with Dermatophilus congolensis
    (Natl Information Documentation Centre, 2022) Simsek, Aynur; Kochan, Akin; Alp, Simten Yesilmen
    Dermatophilus congolensis infection in sheep is manifested as matted tufting, entanglement, hardening and loss of wool, and thickening and itching of the skin, altogether which eventually results in economic losses. We investigated the clinical, hematological and biochemical findings in sheep naturally infected with Dermatophilus congolensis. For this purpose, 35 infected Akkaraman sheep (aged: 3-4 years, belonging to a flock of 200 animals) were referred to Prof Dr. Servet SEKIN Polyclinic of the Dicle University, Veterinary Faculty with various skin problems were included. The infected sheep presented with crusted skin lesions, matted wool tufts, alopecia, and pruritus. Their biochemical serum analyses revealed increased serum alkaline phosphatase, alanine aminotransferase, lactate dehydrogenase, blood urea nitrogen, total protein, albumin and calcium levels, and decreased creatinine, sodium and potassium levels in comparison with the control animals. The infected animals were treated with parenteral amoxicillin/clavulanic acid (Synulox (R)-Zoetis; 8.75 mg/kg body weight, i.m., once daily for 5 days) and local povidone-iodine (Batimer (R)-Esmira) administration, and observed for response to the treatment regimen. We found that Dermatophilus congolensis infection causes skin and wool damage in sheep, along with alteration in the serum biochemical parameters.
  • [ X ]
    Öğe
    Cryptosporidium spp. in Dogs - Prevalence and Genotype Distribution
    (Univ Fed Rio Grande Do Sul, 2023) Celik, Ozgur Yasar; Kochan, Akin; Celik, Burcak Aslan; Ayan, Adnan; Akyildiz, Gurkan; Kilinc, Ozlem Orunc; Ercan, Kerem
    Background: Cryptosporidium spp. is a zoonotic protozoan parasite that affects the gastrointestinal tract of humans and animals. The disease can cause acute and chronic diarrhoea and even death in both humans and animals. In this study, it was aimed to determine the prevalence and genotype distribution of Cryptosporidiosis in shelter dogs in Diyarbakir province located in the Southeastern Anatolia Region of Turkey. Materials, Methods & Results: The animal material of the study consisted of 100 dogs of different breeds and sexes. Faecal samples were collected from the rectum with disposable latex gloves and placed in individual sample containers. All of the samples were examined for Cryptosporidium spp. by Kinyoun Acid Fast and Nested PCR methods. In the Kinyoun Acid Fast staining method, firstly, smear preparations were prepared from fresh faecal samples, fixed in pure methanol for 1 min and allowed to dry. The slides were kept in Kinyoun Carbol-Fuxin for 5 min, dipped in 50% ethyl alcohol, shaken, washed in tap water, kept in 1% sulphuric acid for 2 min and washed in tap water. The slides were kept in methylene blue for 1 min, washed in tap water and allowed to dry. After drying, immersion oil was dripped and examined under a microscope at 100 magnification. DNA extraction was performed from all samples using GeneMATRIX Stool DNA Purification Kit according to the manufacturer's protocol. After Nested PCR analysis was performed. In the PCR step, primers 5'-TTCTAGAGCTAATACATGCG-3' and 5'- CCCATTTCCTTCCTTCGAAACAGGA-3' were used to amplify the 1325 bp gene region. In the nested PCR step, primers 5'- GGAAGGGTTGTATTTATTTATTAGATAAAG-3' and 5'-AAGGAG-TAAGGAACAACCTCCA-3' were used to amplify the 826-864 bp gene region. As a result of both methods, a prevalence of 3% was determined. The infection rate was higher in males (3.57%) than females (2.27%) and in younger than 1 year (5.56%) than in older than 1 year (1.56%). The DNA sequences obtained from the sequence analysis of 3 positive PCR samples were analysed in BioEdit software. A phylogenetic tree was constructed with the data set created by using the 18s rRNA gene sequences obtained from the NCBI genbank database and the DNA sequences obtained as a result of the study, and it was shown which Cryptosporidium species the study samples were related to. Today, many Cryptosporidium species have been identified and most of these species have host adaptation. Although C. canis is the most common species in dogs, C. muris, C. meleagridis, and C. parvum have also been detected. Among these species, C. parvum is recognized as a zoonotic species infecting a wide range of mammals. In this study, DNA sequencing of nested PCR positive samples revealed that 3 samples were zoonotic C. parvum. Discussion: This suggests that dogs may be a reservoir for zoonotic transmission of Cryptosporidium. Consequently, it is recommended that people should be informed about the potential for transmission of this protozoan to humans and animals and that control programmes should be implemented, including the prevention of free entry of stray dogs into public places and homes.
  • [ X ]
    Öğe
    Diaphragmatic hernia in a sheep - a case report
    (Univ Zagreb Vet Faculty, 2018) Simsek, Aynur; Yaman, Turan; Icen, Hasan; Kochan, Akin
    A two-year-old sheep was referred to the clinics of the Department of Internal Medicine of Dicle University, Faculty of Veterinary Medicine, with signs of anorexia, abdominal tympany and constipation. The clinical examination of the animal revealed the presence of respiratory failure associated with weaker lung sounds on the right side of the body when compared to the left side. There were no ruminal movements, and when the rumen was probed with a stomach tube, no ruminal content was obtained. The animal was euthanized upon the request of the owner, and a necropsy was performed. At necropsy, it was observed that the left hepatic lobe had protruded into the thoracic cavity through a defect in the diaphragm, and that a diaphragmatic hernia had developed.
  • [ X ]
    Öğe
    Serum mineral levels and haematobiochemical parameters in buffalo calves with allotriophagy (pica syndrome)
    (Polish Soc Veterinary Sciences Editorial Office, 2023) Kochan, Akin; Simsek, Aynur; Arica, Enes
    Pica is defined as a depraved or abnormal appetite characterized by licking and eating non-nutritive substances. The aim of this study was to report changes in the serum mineral levels and haematobiochemical parameters of buffalo calves with pica. The study included 30 buffalo calves exhibiting pica behaviour, such as licking stall walls or eating wool and non-food items (pica group), and 6 buffalo calves without the signs of pica behaviour (control group). The age of both groups ranged between 1 and 6 months. In the pica group, the haematological analysis revealed a decrease in red blood cells (P < 0.001) and red cell distribution width (P < 0.05) as well as an increase in the mean corpuscular volume and mean corpuscular haemoglobin (P < 0.001), whereas the biochemical parameters were characterized by an increase in aspartate aminotransferase (P < 0.01), alanine aminotransferase (P < 0.001) and lactate dehydrogenase (P < 0.05) levels and a decrease in creatinine and glucose (P < 0.01) levels. Furthermore, the pica group showed a significant decrease in serum manganese, iron, zinc, selenium (P < 0.001) and copper (P < 0.01) concentrations. In conclusion, it was determined that deficiencies in the levels of serum iron, copper, zinc, manganese and selenium play an important role in the aetiology of pica in buffalo calves.