Yazar "Kiyma, Z." seçeneğine göre listele
Listeleniyor 1 - 12 / 12
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Early and efficient pregnancy diagnosis in ewes: expression profile of ISG15 mRNA in peripheral blood leucocytes compared to transrectal ultrasonography(Indian Counc Agricultural Res, 2017) Kiyma, Z.; Kose, M.; Kaya, M. S.; Atli, M. O.The objective of this study was to compare the measurement of one of interferon-tau stimulated genes (ISGs; ISG15) expression in peripheral blood leukocytes (PBLs) with transrectal ultrasonography (TUSG) for early pregnancy detection in ewes. Ewes (46) were examined 18 and 25 days after mating by TUSG. PBL samples were collected from 16 pregnant ewes on the day of mating (d0) and on days 15 and 18 after mating. Total RNAs were extracted from PBLs and converted to cDNA. ISG15 mRNA expression was detected using qPCR in triplicate. All pregnancies were confirmed by birth. In TUSG examinations on day 18, the embryonic vesicle and embryonic vesicle plus the embryo were visible for 14 and 32 ewes, respectively, of the 46 total. Both the embryonic vesicle and embryo were detected in 42 ewes on day 25. Four ewes out of 46 with a visible embryonic vesicle on day 18 were detected as non-pregnant on day 25. While significant differences in ISG15 mRNA expression levels of PBLs were detected in 13 of 16 ewes on day 15, ISG15 mRNA levels for all ewes were significantly different on day 18, compared with levels on the day of mating. The results suggested that profiles of ISG15 mRNA can provide pregnancy detection earlier than TUSG.Öğe Early pregnancy regulates genes of hyaluronan system in ovine endometrium(Wiley-Blackwell, 2016) Kiyma, Z.; Hitit, M.; Kose, M.; Kaya, M. S.; Kirbas, M.; Dursun, S.; Atli, M. O.[Abstract Not Available]Öğe Early pregnancy regulates Toll-like receptors at mRNA level in ovine peripheral blood leucocytes(Wiley-Blackwell, 2014) Kaya, M. S.; Kose, M.; Aydilek, N.; Karahan, S.; Kiyma, Z.; Guzeloglu, A.; Atli, M. O.[Abstract Not Available]Öğe Expression and localization of Toll-like receptor 4 in ovine endometrial and trophoblastic tissues at early pregnancy(Wiley-Blackwell, 2015) Atli, M. O.; Kiyma, Z.; Kose, M.; Kaya, M.; Kaya, M. S.; Yaman, T.; Guzeloglu, A.[Abstract Not Available]Öğe Expression of interferon stimulated genes (ISGs) is extended to pituitary in early pregnant ewes(Wiley-Blackwell, 2016) Ozel, C.; Kose, M. O.; Atli, M.; Hitit, M.; Kiyma, Z.; Kaya, M. S.; Sen, G.[Abstract Not Available]Öğe Expression profile of interferon tau-stimulated genes in ovine peripheral blood leukocytes during embryonic death(Elsevier Science Inc, 2016) Kose, M.; Kaya, M. S.; Aydilek, N.; Kucukaslan, I.; Bayril, T.; Bademkiran, S.; Kiyma, Z.Early and efficient detection of embryonic death (ED) has a valuable impact as important as early pregnancy diagnosis in ruminants. Among early pregnancy diagnosis methods, detection of the expression of interferon tau stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) is well documented in cows and ewes. Therefore, we hypothesized that the expression profile of ISGs in PBLs might also be useful for detecting ED in these animals. For this purpose, pregnant ewes were used as an experimental model. Pregnancy was detected on Day 18 after mating by transrectal ultrasonography. Pregnant ewes were divided into a control group (sham injection on Day 18, n = 10) and ED group (treated with 75 mu g synthetic PGF(2 alpha) on Day 18, n = 12). PBLs and plasma were collected on Days 0 (mating day), 15, 18, 19, 20, 21, 23, and 25 by jugular venipuncture. Total RNA was isolated from PBLs. ISGs expression levels were determined by real-time polymerase chain reaction in triplicate. Electrochemiluminescence immunoassay was used to measure progesterone (P-4) levels in plasma. In the ED group, the P-4 level declined to less than 1 ng/mL on Day 19 and remained at a low level until the end of the study. Compared with that on Day 0, receptor transporter protein 4 (RTP4) and ISG15 expression was upregulated on Day 15 and remained high until Day 21 in both groups, and RTP4 and ISG15 mRNA levels were attenuated on Days 23 and 25 only in the ED group (P < 0.001). Myxovirus resistance 1 expression was upregulated on Day 15 and remained high until Day 23 in both groups, but was attenuated on Day 25 in the ED group (P < 0.05). The B2-microglobulin mRNA level did not change significantly during the study in either group. These results indicate that the decline in P-4 concentration was an immediate response to PGF(2 alpha) and that the embryo may have survived longer than the CL on the basis of the extended period of ISGs expression. This suggests that the absence of P-4 could be the reason for ED rather than a direct effect of PGF(2 alpha). In conclusion, the expression of ISGs, including ISG15, RTP4, and myxovirus resistance 1, but not B2-microglobulin, in PBLs may serve as a marker of ED. (C) 2016 Elsevier Inc. All rights reserved.Öğe Expression profile of receptor transporter protein 4 (Rtp4) mRNA in ovine peripheral blood leucocytes (PBLs) during early embryonic death (EED)(Wiley-Blackwell, 2014) Kose, M.; Kaya, M. S.; Aydilek, N.; Kucukaslan, I.; Bayril, T.; Bademkiran, S.; Kiyma, Z.[Abstract Not Available]Öğe Investigation of Interferon stimulated genes (ISGs) simultaneously in endometrium, corpus luteum (CL) and peripheral blood mononuclear cells (PBMCs) in early pregnant ewes(Wiley-Blackwell, 2015) Kiyma, Z.; Kose, M.; Ozel, C.; Hitit, M.; Atli, M. O.; Guzeloglu, A.; Kaya, M.[Abstract Not Available]Öğe Investigation of microRNA biogenesis at mRNA level in bovine corpus luteum and endometrium during the oestrous cycle(Wiley-Blackwell, 2013) Hitit, M.; Atli, M.; Kurar, E.; Kose, M.; Guzeloglu, A.; Kaya, M.; Kiyma, Z.[Abstract Not Available]Öğe Investigation of nuclear factor erythroid 2-related factor 2 (NRF2) target genes in the ovine endometrium during early pregnancy(Wiley-Blackwell, 2016) Sen, G.; Ozel, C.; Kose, M.; Atli, M.; Kaya, M.; Kirbas, M.; Kiyma, Z.[Abstract Not Available]Öğe miRNAs expression during the peri-attachment period of pregnancy in the ovine endometrium(Wiley-Blackwell, 2016) Kose, M.; Ozel, C.; Guzeloglu, A.; Kaya, M. S.; Kirbas, M.; Dursun, S.; Kiyma, Z.[Abstract Not Available]Öğe Preliminary investigation of microRNAs in ovine endometrium(Wiley-Blackwell, 2015) Kiyma, Z.; Hitit, M.; Ozel, C.; Sen, G.; Kose, M.; Guzeloglu, A.; Kaya, M.[Abstract Not Available]
