Yazar "Ketani M.A." seçeneğine göre listele
Listeleniyor 1 - 6 / 6
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Effects of tamoxifen administration in rat vaginas: An ultrastructural and light microscopy study(2002) Ketani M.A.; Ketani Ş.; Kaloğlu C.; Güney B.Background: Tamoxifen (TAM) inhibits the initiation of carcinogen induced rat mammary tumours and is administrered for extended periods after the initiation of carcinogenesis. It is also a widely used treatment for breast and gynaecological cancer. Objective: In this study, we aimed to investigate tamoxifen (TAM) administration on vagen development in rats. Material & Methods: Twenty sexually mature and pregnant Wistar albino rats were chosen as the animal model. They were divided into two groups. Group I: Control group, Group II: Tamoxifen applied (between gestational day 16 and 21 days); 100 ?g tamoxifen citrate (TAM) in 0.05 ml saline subcutaneously per day/animal. After birth, all female rats were sacrificed on the 60th day and were taken vaginal tissue. Transmission electron microscopy and light microscopy have been used to study changes to the vaginal epithelium. Results: A statistically significant reduction of birth body weight was noted in the experimental group of rats when compared to the control group (P<0.05). We saw increase in the thickness of the epithelium layer and irregularity and disappearance of microscopic papilla, cytoplasmic vacuolation in cells of the surface layer, thin and irregular basal membrane, lateral junction of cells were destroyed in the TAM treated groups. In conclusion, neonatal tamoxifen administration affects vagina epithelium and lead to decreasing birth body weight and vaginal adenosis.Öğe Histochemical Characterization of Mucins in the Cervix and Uterus of Cows during the Sexual Cycle(University of Dicle, 2022) Aydin N.; Ketani M.A.; Topaloglu U.; Celenk F.; Bayram B.; Sagsoz T.In all mammals, including humans, the mucosal surfaces of the gastrointestinal, respiratory, genital and urinary systems are the gateway for infectious agents. Against these factors cause diseases, mucosal surfaces are covered with a slimy substance called mucus, which acts as a physiochemical barrier. The study was conducted to investigate the types and distributions of mucins secreted in the bovine uterus and cervix during the follicle and luteal phases of the sexual cycle. For this purpose, organs of 23 healthy Holstein cows were used. After the tissues were fixed in 10% neutral formalin, paraffin was embedded following routine histological procedures. In both cervix and the uterus, the mucins were more dominant in luminal epithelial and gland epithelial cells during the luteal phase than those of the follicular phase. Periodic acid Schiff (PAS) and Aldehyde Fuchsin (AF) were dominant in the cervix during the luteal phase. Uterinal gland epithelial cells reacted more intensely in both follicular and luteal phases in the Best's Carmine staining. In Periodic acid-Phenilhydrazine-Schiff (PAPS) staining, there was no staining of gland epithelial cells in the uterus during sexual cycle. As a result, bovine cervix and uterine mucosae secreted neutral, sulphated acid mucins, Nacetyl sialomucins and glycogen at varying densities during sexual cycle, while carboxylated acid mucins were not present. Considering the physiological functions of the mucus, we can say that they are also involved in creating a protective barrier in the cervix and uterine mucosa and during the implantation periods © 2022, Journal of International Dental and Medical Research. All Rights Reserved.Öğe Histological changes in the gingrval tissues of rats subjected to surgical extraction of the adrenal glands(2009) Dag A.; Ketani M.A.; Zengingul A.I.; Uysal E.; Baskan Z.Hormones are specific regulatory molecules that have potent effects on the major determinants of the development and the integrity of the skeleton and oral cavity also including periodontal tissues. The aim of this study, is to investigate the effect of adrenalectomy on rat gingival tissue. Twenty-eight mature Wistar albino female and male rats divided randomly into 2 groups and in one group adrenalectomy was performed. After 21 days recovery period, Control and Experimental groups were sacrificed under Ketamin HCi anesthesia and clinical and histological observations were made. Microscopic findings, revealed significant changes in adrenalectomized rats. Hypertrophy and thickness of gingival epitelium was apparent in the gingival epithelium of ADX group. Increase in mitotic activity in stratum basale layer of gingival epitelium and increase in keratinisaton layer were observed in adrenalectomized rats. A decrease in collagen fibrils and irregularity of papilla was seen in adrenalectomized rats. Our findings demonstrate the importance of adrenal glands in the development of gingival tissue and suggest that adrenal glands deficiency is one mechanism, by which gingival disorder susceptibility may be increased. © Medwell Journals, 2009.Öğe Immunohistochemical detection of estrogen and progesteron receptors in the bovine uterus and their relation to serum sex steroid hormone levels during the follicular and luteal phase(2009) Guney Saruhan B.; Sagsoz H.; Ketani M.A.; Akbalik M.E.; Ozyurtlu N.In this study, we tried to show the expression patterns of the steroid receptors in the bovine endometrium during the follicular and luteal phase. Samples of both uterus and blood were obtained from 30 adult, healty bovine at the moment of slaughter at a local slaughterhouse. Immunohistochemistry was performed by using rabbit polyclonal antibodies against the estrogen receptor (ER) and mouse monoclonal antibodies against the progesterone receptor (PR). In general, most of the uterine cells were stained positive but with a different intensity. During follicular phase, both ER and PR were obviously strong in the epithelia and the myometrium. For the glandular epithelium (GE), all GE cells were stained positive for ER and PR. When we compared both receptors during lutheal phase, a stronger intensity was observed in all compartments for PR, especially in the myometrium and surface-glandular epithelium. To summarize, the results from this study showed that both ER and PR might be regulated by the same mechanisms in some compartments and at specific stages of the oestrous cycle, and that each compartment of the uterus had a different expression of ER and PR which could accord with their different roles in reproductive physiology.Öğe Immunohistochemical localisation of oestrogen and epidermal growth factor receptors of the bitch uterus in the sexual cycle(2010) Ozyurtlu N.; Sagsoz H.; Saruhan B.G.; Zonturlu A.K.; Ketani M.A.; Akbalik M.E.The localisation of oestrogen (ER) and epidermal growth factor receptors (EGFR) in the various cell types of the bitch uterine was determined. In this study, 23 adult, healthy crossbred bitches brought to the clinic for ovariohisterectomy were used. ER and EGFR positive staining was detected in all cell types of the uterus. A distinct staining was seen in the luminal and glandular epithelium; while stromal and myometrial cells showed weak or moderate staining. The endothelial and smooth muscle cells of the vessels in the endometrium and myometrium sometimes appeared positive. No staining was observed in the mesothelium. The results of this study suggested that ER and EGFR were expressed at various levels in different cell types of bitch uterus. In light of the previous studies, and data of the presented investigations, it may be necesssary to elicit the harmonious proliferation and differentiation of epithelial and stromal cells that are considered essential for the preparation of the uterus for implantation.Öğe Intravesical oxybutynin application: Ultrastructural effects on bladder epithelium(1999) Ersay A.; Ketani M.A.; Nergiz Y.; Demirtas O.C.; Akkus M.; Otludil B.Intravesically applied oxybutynin, rapidly absorbs into the bloodstream, additional to profound local effect. Currently morphologic effects of oxybutynin on local bladder tissue relatively well established at light microscopic level, but not ultrastructural level. Thirty New Zealand White female rabbits were catheterized daily and intravesical instillation were performed with whether l mg/kg oxybutynin solution or saline for 30 days. The local effects of the drug on bladder epithelium at electron microscopic level were examined comparing with saline administration. Urinary tract infection (UTI) incidence were similar in both saline and oxybutynin groups (9 vs. 10 of 15 animals respectively) (p>0.05). Interestingly, in 4 of 5 animals that received oxybutynin and never had UTI during the study, separation of zonula occludens intercellular junction was determinated by Jeol electron microscope. This observation can explain rapid absorption of oxybutynin through the bladder into the blood stream. Oxybutynin can damage bladder surface epithelium at ultrastructural level. This effect may lead to increase absorption of it, but not result in higher incidence of bacterial infection.
