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Öğe Can Paricalcitol Increase the Effectiveness of N-Acetylcysteine in Contrast Induced Acute Kidney Prophylaxis in Rats? A Biochemical and Histopathological Study(Soc Chilena Anatomia, 2022) Yildirim, Yasar; Bahadir, Veysi; Aydin, Emre; Aydin, Fatma Yilmaz; Yilmaz, Zulfukar; Ketani, Aydin; Kaplan, Ibrahim& nbsp;N-Acetylcysteine (NAC) is used for contrast induced acut kidney injury (CI-AKI) prophylaxis because of its antioxidant effects. Paricalcitol, which has reno-protective effects, is likely to provide a more effective prophylaxis when added to NAC treatment. The study was designed based on this hypothesis. The study was organised to include 4 groups each consisting of 7 rats. Group 1 was the control group, and Group 2 included rats with CI-AKI. Rats in Group 3 were administered NAC at a dose of 100 mg/kg via oral gavage once a day for 5 days. Rats in group 4 were administered paricalcitol at a dose of 0.4 mcg/kg once a day for 5 days in addition to NAC. CI-AKI was induced after the treatments in both groups. The study was terminated on the sixth day. Samples were collected from the rats & rsquo; sera and kidney tissues to study oxidant and antioxidant parameters; kidney function tests were also studied. There were significant differences between the contrast nephropathy group (Group 2) and NAC and NAC+paricalcitol groups with respect to serum urea and creatinine levels. When the same groups were compared regarding oxidant (TOS-MDA) and antioxidant (TAC-Paraoxonase) parameters, we observed that the oxidant parameters increased in serum and kidney tissue samples with NAC use, and that effect was strengthened by the addition of paricalcitol to NAC treatment. However, despite increased antioxidant effectiveness, we observed no decrease in urea and creatinine levels when paricalcitol was added for CI-AKI in rats. There was no significant difference between Group 3 and Group 4. Paricalcitol provides a more potent antioxidant effect in both serum and kidney tissue samples when added to NAC treatment in rats with CI-AKI. Despite increased antioxidant parameters, however, paricalcitol does not provide a significant decrease in urea and creatinine levels.Öğe Effect of 50-Hz 1-mT magnetic field on the uterus and ovaries of rats (Electron microscopy evaluation)(Int Scientific Information, Inc, 2006) Aksen, Feyzan; Akdag, M. Zulkuf; Ketani, Aydin; Yokus, Beran; Kaya, Abdurrahman; Dasdag, SuleymanBackground: The aim of this study was to investigate the effect of extremely low frequency magnetic fields (ELFMF) on the uterus and ovary of rats. Material/Methods: Forty-eight female Wistar albino rats were divided into two groups, one for 50 and the other for 100 days of exposure. Each group was further divided into two groups, one sham exposed (n=12) and the other the experimental group (n=12). The experimental rats were exposed to 50-Hz 1-mT ELFMF for three hours/day for 50 or 100 days. The sham groups of rats were kept under the same circumstances without applying ELFMF. Electron microscopic examination was performed to evaluate the ovaries and uterus. Results: Ultrastructural dissolution, decrease in cell organelles, cavities in cells, heterochromative appearance, and typical structural loss of the nucleus were observed in germinal epithelial cells of the rat ovaries in the 50-days ELFMF exposure group. Ultrastructural alterations in germinal epithelium and tunica albuginea of ovaries, irregularity in nucleus and nucleolus, increase in lipid vacuoles of cell cytoplasm and reduction in organelles were observed in rat ovaries in the 100-days ELFMF exposure groups. Similar alterations were observed in uterus. Malondialdehyde concentration (MDA) of the ovaries and uterus increased in rats of the two exposure groups (p < 0.001). Conclusions: The results of the study showed that 50 and 100 days of exposure to a 1-mT ELFMF can cause alterations at the cellular level and in MDA concentration.Öğe The effect of low-level laser therapy on the healing of hard palate mucosa and the oxidative stress status of rats(Wiley, 2014) Firat, Ela Tules; Dag, Ahmet; Gunay, Ahmet; Kaya, Beyza; Karadede, Mehmet Irfan; Kanay, Berna Ersoz; Ketani, Aydin[Abstract Not Available]Öğe Effect of lymphedema on the recovery of fractures(Elsevier, 2007) Arslan, Hueseyin; Uludag, Abuzer; Kapukaya, Ahmet; Gezici, Ayten; Bekler, H. Ibrahim; Ketani, AydinBackground. Lymphedema delays the healing of any wound by negatively affecting its inflammatory period. Whether it affects bone healing in a similar negative manner is unknown. Therefore, we experimentally investigated the effect of lymphedema on fracture recovery. Methods. We used thirty 200- to 250-g Sprague-Dawley rats for the experiment. The rats were randomly divided into two groups of 15 rats each for the experimental lymphedema and control groups. Lymphedema development was confirmed by measuring the circumference and diameter of the extremities together with lymphoscintigraphy. Twenty days after the development of lymphedema, a fracture model was created in both groups in the right tibia with mid-diaphyseal osteotomy and fixing with an intramedullary Kirschner wire. After 6 weeks, all rats were sacrificed and the callus tissue that formed along the osteotomy was compared between groups with respect to radiographic, histological, and biomechanical characteristics. Results. The three-point bending test yielded an average stiffness value of 1227 N/mm (n = 6) in the control group and 284 N/mm (n = 7) in the experimental lymphedema group (P < 0.05). At the end of week 6, radiographic evaluation showed that solid knitting was obtained in the control group, whereas in the lymphedema group delayed or no knitting was observed. In the control group, histological investigation revealed normal callus morphology. Trabecular bone was normal and osteoblast and osteoclast activity was clearly evident. The bone was stained homogeneously with hematoxylin and eosin, and ossification was within normal limits. In the lymphedema group, however, the histological appearance was mostly that of scar tissue. In addition, osteoblast and osteoclast activity was much less visible or absent. Conclusions. Lymphedema negatively affected bone healing in rats. However, the mechanism of this negative effect and its occurrence in humans are still unknown. Further experimental and clinical studies are needed to support and extend our findings.Öğe Effects of estrogen deficiency on tooth movement after force application: an experimental study in ovariectomized rats(Taylor & Francis As, 2007) Arslan, Seher Guenduez; Arslan, Hueseyin; Ketani, Aydin; Hamamci, OrhanObjective. The aim of this study was to investigate the effects of estrogen deficiency on tooth movement in ovariectomized rats. Material and methods. Forty-two adult female Sprague-Dawley rats were assigned at random to one of the following groups: test group (n=20), ovariectomized rats (or estrogen-deficient rats); control group (n=22), non-ovariectomized rats. Two months after ovariectomy, expansion springs exerting 10 g of force were inserted between the upper central incisors in both groups. The amount of movement was measured daily until tooth movement began and then at intervals of 3 days. The rats were sacrificed 18 days after applying the expansion spring and histomorphometric analysis was performed along the left upper central incisor root towards the apex of the alveolar bone. Results. The amount and speed of movement was observed to be greater in ovariectomized rats. On histomorphometric analysis, osteoblast and osteocyte counts on the pressure side were higher in the non-ovariectomized group than in the ovariectomized group (p < 0.001). In contrast, the osteoclast count was significantly higher (p < 0.01) in the ovariectomized group than in the non-ovariectomized group. The osteoblast and osteocyte counts were significantly higher (p < 0.001) on tension side in the non-ovariectomized group than in the ovariectomized group. Conclusion. Estrogen deficiency increased orthodontic tooth movements but counts of osteoblasts, which are responsible for new bone formation, were lower in regions of tension and of pressure.Öğe The Effects of Low-Level Laser Therapy on Palatal Mucoperiosteal Wound Healing and Oxidative Stress Status in Experimental Diabetic Rats(Mary Ann Liebert, Inc, 2013) Firat, Ela Tules; Dag, Ahmet; Gunay, Ahmet; Kaya, Beyza; Karadede, Mehmet Irfan; Kanay, Berna Ersoz; Ketani, AydinObjective: The biostimulation effects of low-level laser therapy (LLLT) have recently been demonstrated. In this study, we aimed to investigate the effects of LLLT on palatal mucoperiostal wound healing and oxidative stress status in experimental diabetic rats. Materials and methods: Forty-two male Wistar rats that weighed 250-300 g were used in this study. Experimental diabetes was induced in all of the rats using streptozotocin. A standardized full thickness wound was made in the mucoperiosteum of the hard palates of the rats using a 3 mm biopsy punch. The rats were divided into groups: 1 (control group, non-irradiated), and 2 (experimental group, irradiated). Treatment using a GaAlAs laser at a wavelength of 940 nm and at dose of 10 J/cm(2) began after surgery, and was repeated on the 2nd, 4th, and 6th days post-surgery. Seven animals from each group were killed on the 7th, 14th, and 21st day after surgery. Biopsies were performed for the histological analysis and blood samples were collected by cardiac puncture for biochemical analysis. Results: The histopathological findings revealed reduced numbers of inflammatory cells, and increased mitotic activity of fibroblasts, collagen synthesis, and vascularization in rats in group 2. The total oxidative status was significantly decreased in the laser-treated group on the 21st day. Conclusions: LLLT elicits a positive healing effect on palatal mucoperiostal wounds, and modulates the oxidative status in experimental diabetic rats.Öğe Effects of specific inhibition of cyclooxygenease-2 on kidney in bilateral adrenalectomized rats(Springer, 2007) Ozturk, Hulya; Ozturk, Hayrettin; Gedik, Senol; Uzunlar, Ali Kemal; Ketani, AydinIn the kidney, prostaglandins represent important physiological modulators of renal hemodynamics and salt and water homeostasis. In this experimental study of bilaterally adrenalectomized (ADX) rats, we aimed to investigate whether the administration of selective (celecoxib) inhibitor of COX-2 would alter the morphological and functional changes in rat kidney tissue. Twenty-one male Sprague-Dawley rats weighing 225-250 g were used. The animals were divided into three groups. Group 1 rats (Sham-control, n = 7) did not receive any treatment. In group 2 rats (ADX/Untreated, n = 7), bilateral ADX was performed via a single dorsal incision. In group 3 (ADX/COX-2) rats, the same operation was performed as described for group 2 and then the COX-2 inhibitor celecoxib was administered by gavage for a period of 7 days. On the 7th day of the study, renal function was assessed by measurements of blood urea nitrogen (BUN) and serum creatinine levels. Biopsies were obtained from the remaining left kidneys before killing the rats. There was no significant difference in the BUN and creatinine values between the groups. In ADX/Untreated group, capillary congestion in glomerule, inflammation, hemorrhage and congestion in intertubular area, and cytoplasmic vacuolation in renal tubules was observed. Mild damage was observed in the ADX/COX-2 group. The number of macrophages was significantly decreased in ADX/COX-2 group when compared to ADX/Untreated group (P < 0.0001). Our study indicates that celecoxib may be an important factor affecting renal morphological changes after the bilaterally ADX.Öğe EVALUATION OF THE ANTIOXIDANT AND RENOPROTECTIVE EFFECTS OF ELLAGIC ACID ON ISCHEMIA/REPERFUSION INDUCED NEPHROPATHY IN RATS(Oxford Univ Press, 2014) Yildirim, Yas Ar; Aba, Ozlem; Yilmaz, Zulfukar; Kadiroglu, Ali Kemal; Yilmaz, Mehmet Emin; Gul, Mesut; Ketani, Aydin[Abstract Not Available]Öğe EVALUATION OF THE ANTIOXIDANT AND RENOPROTECTIVE EFFECTS OF ELLAGIC ACID ON ISCHEMIA/REPERFUSION INDUCED NEPHROPATHY IN RATS(Carbone Editore, 2014) Yildirimi, Yasar; Adiyaman, Ozlem Aba; Yilmaz, Zulfukar; Kadiroglu, Al Kemal; Yilmaz, Mehmet Emin; Gul, Mesut; Ketani, AydinAims: Renal ischemia-reperfusion (I/R) injury is one of the important cause of acute kidney injury (AKI). Reactive oxygen species and inflammatory cytokines play major role in the pathogenesis of IR injury. Ellagic acid (EA), a phenolic compound, have shown to exert antioxidants, anti-inflammatory, anticarcinogenic, antihyperlipidemic effects. We aim to evaluate, the effect of EA on renal I/R induced nephropathy in rats. Materials and methods: Twenty-eight male Sprague-Dawley rats were divided into four groups; control, control + EA, I/R, and EA+I/R. EA (85 mg/kg, perorally) was administered 30 min prior to the ischemia. Rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 60 min of reperfusion. Both groups were subsequently studied by renal function tests, oxidant and antioxidant parameters, and kidney histology. Results: Serum/kidney TAC, NO and paraoxonase levels were significantly higher, while serum urea and creatinine, serum/kidney MDA and TOS were significantly lower in EA+I/R group compared to I/R group (p<0.05). Histopathologic examination revealed that the severity of damage was significantly lower in the EA+I/R group compared to the I/R alone group. Conclusion: Administration of EA appears to have beneficial effects on I/R induced renal injury by reducing oxidative stress, thus preventing histological injuries and bringing about an improvement in renal function.Öğe Pretreatment with Paricalcitol Attenuates Oxidative Stress in Renal lschemia Reperfusion-Induced Nephropathy in Rats(Sci Printers & Publ Inc, 2020) Aydin, Fatma Yilmaz; Yilmaz, Zulfukar; Yildirim, Yasar; Aydin, Emre; Ketani, Aydin; Bahadir, Veysi; Kaplan, IbrahimOBJECTIVE: To investigate whether paricalcitol could ameliorate kidney injury due to ischemia reperfusion (I/R) in an experimental study. STUDY DESIGN: Rats were divided into 4 groups: control, paricalcitol, I/R, and paricalcitol +I/R, each containing 7 animals. Intraperitoneal 0.3 mu g/kg paricalcitol was administered to rats once a day for 5 consecutive days in the paricalcitol and paricalcitol+1/R groups. After right nephrectomy, rats were exposed to ischemia/ reperfusion on day 6 in the paricalcitol+1/R and I/R groups. Oxidant and antioxidant parameters, kidney function tests, and histology were investigated. RESULTS: Serum urea and creatinine levels exhibited a significant decrease in rats treated with paricalcitol before I/R as compared to rats exposed just to I/R. In a comparison of the paricalcitol +1/R group with the I/R group, serum total oxidant status (TOS) levels decreased significantly; serum total antioxidant capacity (TAC) and nitric oxide levels, however, increased significantly with paricalcitol administration. Malondialdehyde and TOS levels of kidney tissue were significantly lower, whereas TAC and paraoxonase levels were higher in the paricalcitol +I/R group than in the I/R group. Renal tissue injury scores were found to be significantly higher in the I/R group than in the paricalcitol+ I/R group. CONCLUSION: Pretreatment with paricalcitol was detected to be renoprotective by decreasing renal injury related with renal I/R, which was assessed by improved renal function and histopathologii.Öğe Protective effect of Diyarbakir watermelon juice on carbon tetrachloride-induced toxicity in rats(Pergamon-Elsevier Science Ltd, 2011) Altas, Sevcan; Kizil, Goksel; Kizil, Murat; Ketani, Aydin; Haris, Parvez I.The present study was designed to evaluate the effect of Diyarbakir watermelon (Citrullus lanatus cv. Surme) juice on lipid peroxidation states in rat liver, kidney and brain. In vivo administration of carbon tetrachloride (CCl4) once a week for 28 days caused a significant elevation of serum markers of liver damage, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TB) and decrease in albumin when compared to the control group. However, administration of carbon tetrachloride along with watermelon juice or ursodeoxycolic acid (UDCA) significantly reduces these changes. Increased lipid peroxide (LPO) level was observed in the liver, kidney and brain tissues after CCl4 administration. However, watermelon juice and UDCA treatment prevented the increase in LPO. The results indicated that watermelon juice protects the liver, kidney and brain tissues from experimental CCl4 toxicity in rats and that the protective effect of watermelon juice may be due to its antioxidant activity and inhibition of lipid peroxide formation. In conclusion, present study reveals biological evidence that supports the use of watermelon juice in the treatment of chemical-induced hepatotoxicity. (C) 2011 Elsevier Ltd. All rights reserved.Öğe Protective effects of coumarin and coumarin derivatives against carbon tetrachloride-induced acute hepatotoxicity in rats(Elsevier Gmbh, Urban & Fischer Verlag, 2011) Bilgin, Hakki Murat; Atmaca, Mukadder; Obay, Basra Deniz; Ozekinci, Selver; Tasdemir, Ezel; Ketani, AydinThe comparison of the antioxidant activity of some coumarins with their molecular structure is well determined. However, the protective function of coumarins with various chemical structures against liver toxicity has not yet been well established. Therefore, the aim of this study was to evaluate the possible cytoprotective properties of coumarin and some coumarin derivatives against CCl(4) (carbon tetrachloride)-induced hepatotoxicity. Coumarin (1,2-benzopyrone) and coumarin derivatives esculetin (6,7-dihydroxycoumarin), scoparone (6,7-dimethoxycoumarin) and 4-methylumbelliferone (7-hyroxy-4-methyl) were examined for their protective effect against CCl(4)-induced hepatotoxicity in Male Sprague-Dawley rats. A single toxic dose of CCl(4) (1.25 ml kg(-1), orally) produced liver damage in rats, seen histologically as centrilobular necrosis. Administration of CCl(4) increased serum enzyme levels of aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP). Pretreatment of rats with esculetin (31.15 mg kg(-1), orally) and scoparone (35 mg kg(-1), orally) significantly prevented CCl(4)-induced increase in serum enzymes, whereas 4-methylumbelliferone (35 mg kg(-1)) and coumarin (30 mg kg(-1)) had no effect against CCl(4)-induced rise in serum enzymes. Morphological findings were consistent with the plasma transaminase observations. Among the coumarin analogs, esculetin, which possesses orthodihydroxy coumarins, showed the strongest protective effect against CCl(4)-induced liver damage, followed by scoparone, 4-methylumbelliferone and coumarin, respectively. The results of this study indicate that the chemical structures of coumarins play an important role in the prevention of liver toxicity. (C) 2010 Elsevier GmbH. All rights reserved.Öğe The protective effects of paricalcitol on renal ischemia reperfusion induced lung injury(European Respiratory Soc Journals Ltd, 2018) Yilmaz, Sureyya; Yilmaz, Zulfukar; Kadiroglu, Ali Kemal; Bahadir, Veysi; Kaplan, Ibrahim; Ketani, Aydin; Yilmaz, Engin Deniz[Abstract Not Available]Öğe Protective Effects of Paricalcitol on Renal Ischemia/Reperfusion-Induced Lung Injury(Sci Printers & Publ Inc, 2021) Yilmaz, Sureyya; Yildirim, Yasar; Kadiroglu, Ali Kemal; Bahadir, Veysi; Aydin, Emre; Aydin, Fatma Yilmaz; Ketani, AydinOBJECTIVE: Acute kidney injury (AKI) is a common and important clinical challenge, and renal ischemia/reperfusion (I/R) injury is the major reason of AKI. Renal I/R can lead to lung injury, which is associated with increased mortality. This study was designed to evaluate whether paricalcitol may protect against lung injury following renal I/R injury via its antioxidant properties. STUDY DESIGN: Rats (n=7 per group) were divided into 4 groups: control, paricalcitol, I/R, and paricalcitol + I/R. Rats received daily intraperitoneal injection of paricalcitol (0.3 mu g/kg) for 5 days in the paricalcitol and paricalcitol + I/R groups. On day 6, rats were subjected to I/R injury (60 minutes of left renal artery occlusion followed by 60 minutes of reperfusion) after right nephrectomy. Renal function tests, oxidant and anti-oxidant parameters, and lung histology of both groups were examined. RESULTS: Pretreatment of rats with paricalcitol in the paricalcitol + I/R group significantly decreased serum urea and creatinine levels as compared with the I/R group (p < 0.05). Malondialdehyde (MDA) and total oxidant status (TOS) levels were significantly increased in serum and lung tissue of the I/R group as compared with the control and paricalcitol groups (p < 0.05). Rats treated with paricalcitol prior to I/R injury exhibited significant reduction in terms of serum and lung tissue TOS and MDA levels and significant increase in terms of serum and lung tissue nitric oxide and total antioxidant capacity levels (p < 0.05). The lung histopathological scores were significantly higher in the I/R group as compared with the paricalcitol + I/R group (p < 0.05). CONCLUSION: Paricalcitol may ameliorate renal I/Rinduced lung injury by attenuating oxidative stress.Öğe The protective role of pomegranate juice against carbon tetrachloride-induced oxidative stress in rats(Sage Publications Inc, 2014) Pirinccioglu, Mihdiye; Kizil, Goksel; Kizil, Murat; Kanay, Zeki; Ketani, AydinMost pomegranate (Punica granatum Linn., Punicaceae) fruit parts are known to possess enormous antioxidant activity. The present study was carried out to determine the phenolic and flavonoid contents of Derik pomegranate juice and determine its effect against carbon tetrachloride (CCl4)-induced toxicity in rats. Animals were divided into four groups (n=6): group I: control, group II: CCl4 (1ml/kg), group III: CCl4+pomegranate juice and group IV: CCl4+ursodeoxycholic acid (UDCA). Treatment duration was 4weeks, and the dose of CCl4 was administered once a week to groups II, III and IV during the experimental period. CCl4-treated rats caused a significant increase in serum enzyme levels, such as aspartate aminotransferase, alanine aminotransferase and total bilirubin, and decrease in albumin, when compared with control. Administration of CCl4 along with pomegranate juice or UDCA significantly reduces these changes. Analysis of lipid peroxide (LPO) levels by thiobarbutiric acid reaction showed a significant increase in liver, kidney and brain tissues of CCl4-treated rats. However, both pomegranate juice and UDCA prevented the increase in LPO level. Histopathological reports also revealed that there is a regenerative activity in the liver and kidney cells. Derik pomegranate juice showed to be hepatoprotective against CCl4-induced hepatic injury. In conclusion, present study reveals a biological evidence that supports the use of pomegranate juice in the treatment of chemical-induced hepatotoxicity.
