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Öğe Changes in blood antioxidant status and lipid peroxidation following distance running(1998) Atmaca, Mukadder; Koçyiğit, Yüksel; Şermet, Abdurrahman; Diken, Hüda; Kelle, MustafaTo examine the effects of increased O2 utilization on blood antioxidant status and lipid peroxidation, ten trained male athletes took part in a 20 km distance race. Venous blood samples were removed before and immediately after completion of the half-marathon. Plasma creatine kinase (CK) activity, an index of muscle damage, increased (p<0.05) after the race but this was not accompanied by changes in thiobarbutiric acid reactive substance (TBARS), which is an index of lipid peroxidation. Plasma cholesterol and uric acid concentrations significantly increased (p<0.01 and p<0.05 respectively) but plasma $alpha$-topocherol, triglyceride, and the total protein levels did not change (p>0.05) after the race. The erythrocyte and leucocyte counts, hematocrit, and hemoglobin levels rose after the race (p<0.05, p<0.001, p<0.05, and p<0.01, respectively). However,the mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration (MCHC) did not change. Erythrocyte superoxide dismutase (SOD) and catalase activities were unaffected by the exercise but total glutathione (GSH) and reduced GSH decreased (p<0.05) after the race. In the post-race samples, a rise in oxidized glutathione (GSSG) was not statistically significant (p>0.05). The result indicate that, when trained athletes run a comparatively short distance sufficient to cause some degree of muscle damage but insufficient to cause increases in the plasma indices of lipid peroxidation, changes in erythrocyte antioxidant status do occur.Öğe Effect of exercise on blood antioxidant status and erythrocyte lipid peroxidation: Role of dietary supplementation of vitamin E(1999) Atmaca, Mukadder; Kelle, Mustafa; Diken, Hüda; Şermet, Abdurrahman; Tümer, CemilWe tested the effects of moderate physical exercise on the blood antioxidant capacity and erythrocyte lipid peroxidation in 21 Wistar albino rats. Erythrocyte superoxide dismutase (SOD) activity increased significantly (p<0.05) in control exercised animals (C-Ex), but catalase activity did not change. SOD activity was decreased by dietary supplementation of vitamin E (p<0.05). In vitamin E supplemented group (E-Ex), catalase activity was reduced in comparision to C-Ex group. Total glutathione (total GSH) level was unaffected by the exercise. However, significant reduction was observed in reduced glutathione (GSH), whereas oxidized glutathione (GSSG) increased (p<0.01 and p<0.001, respectively). In E-Ex animals, total GSH and GSH were increased in comparison to C-Ex group. GSH/GSSG ratio decreased abnormally in both exercised groups (p<0.001). Serum cholesterol and uric acid levels increased significantly after exercise (p<0.05). The susceptibility of eryhrocytes to in vitro peroxidation increased in C-Ex and E-Ex animals (p<0.001 and p<0.01, respectively). Elevated malondialdehyde (MDA) concentrtaion in serum attained statistical significance after exercise. However, this elevation was prevented by vitamin E supplementation. Our results indicated that moderate intensive treadmill running exercise was sufficient to result in muscle damage and increases in the susceptibility of erythrocytes to in vitro peroxidation. In addition, dietary supplementation of vitamin E is able to minimize oxidative damage caused by exercise.Öğe Effect of ghrelin on gastric myoelectric activity and gastric emptying in rats(Elsevier, 2008) Tumer, Cemil; Oflazoglu, Hueda Diken; Obay, Basra Deniz; Kelle, Mustafa; Tasdemir, EzelGhrelin is a recently discovered peptide in the endocrine cells of the stomach, which may stimulate gastric motility via the vagal nerve pathway. However, the mechanism of ghrelin-induced changes in gastrointestinal motility has not been clearly defined. The purpose of this study was to investigate the pharmacological effects of ghrelin on gastric myoelectrical activity and gastric emptying in rats, and to investigate whether cholinergic activity is involved in the effects of ghrelin. The study was performed on Sprague-Dawley rats implanted with serosal electrodes for electrogastrographic recording. Gastric slow waves were recorded from fasting rats at baseline and after injection of saline, ghrelin, atropine, or atropin+ghrelin. Gastric emptying of non-caloric liquid was measured by the spectrophotometric method in conscious rats. Intravenous administration of rat ghrelin (20 mu g/kg) increased not only dominant frequency, dominant power and regularity of the gastric slow wave but also the gastric emptying rate when compared with the control rats (P < 0.01, P < 0.05, P < 0.05, P < 0.001 respectively). These stimulatory actions of ghrelin on both gastric myoelectrical activity and gastric emptying were not fully eliminated by pretreatment with atropine sulphate. These results taken together suggest that ghrelin may play a physiological role in the enteric neurotransmission controlling gastric contractions in rats. (c) 2007 Elsevier B.V. All rights reserved.Öğe Effect of Kefir Consumption on Erythrocyte Osmotic Fragility and Some Haemetological Parameters in Smokers and Non-Smokers(Wiley, 2017) Diken, Huda; Oguz, Zelal; Kaya, Hacer; Demirtas, Berjan; Kelle, Mustafa; Atmaca, Mukadder[Abstract Not Available]Öğe Effect of nitric oxide on phagocytic activity of lipopolysaccharide-induced macrophages(Wiley, 2007) Tumer, Cemil; Bilgin, Hakki Murat; Obay, Basra Deniz; Diken, Huda; Atmaca, Mukadder; Kelle, MustafaAmong the antimicrobial mechanisms associated with macrophages, NO produced by iNOS plays a major role in intracellular killing, but the relationship between NO and phagocytic activity after injection of inflammatory agents into the peritoneal cavity is not clear. The aim of the present study was to investigate the effect of nitric oxide (NO) on macrophage function after treatment with intraperitoneal lipopolysaccharide (LPS) and the role of exogenous L-arginine administration in this event. Six experimental groups and one control group, each consisting of seven Wistar rats were used: Group I: Control; Group II: LPS; Group III: LPS + L-arginine; Group IV: LPS + L-arginine + Aminoguanidine; Group V: LPS + Aminoguanidine; Group VI: L-arginine; Group VII: Aminoguanidine. Macrophage phagocytic activity and total plasma nitrite levels were increased in the LPS group. In the LPS + L-arginine group, both the phagocytic activity and total plasma nitrite levels showed large increases. Administration of aminoguanidine (AG), a specific iNOS inhibitor, abolished macrophage phagocytic activity and total plasma nitrite levels in the LPS and LPS + L-arginine groups. As a result, we showed that NO produced by macrophages has a role not only in intracellular killing, but also in phagocytic activity. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.Öğe Effects of cigarette smoking on blood antioxidant status in short-term and long-term smokers(2001) Şermet, Abdurrahman; Kelle, Mustafa; Deniz, Basra; Tümer, Cemil; Baylan, Yüksel; Diken, HüdaEffects of cigarette smoking on blood antioxidant status in short-term and long-term smokersÖğe The hepatoprotective effect of coumarin and coumarin derivates on carbon tetrachloride-induced hepatic injury by antioxidative activities in rats(Springer, 2011) Atmaca, Mukadder; Bilgin, Hakki Murat; Obay, Basra Deniz; Diken, Huda; Kelle, Mustafa; Kale, EbruCoumarins are a vast group of natural compounds and some of them possess antioxidant activities. The comparison of the antioxidant activity of some coumarins with various chemical molecular structure has not been investigated in previous studies. Therefore, this study was aimed to investigate the hepatoprotective effect against carbon tetrachloride (CCl4) -induced hepatic injury by coumarin (1,2-benzopyrone) and coumarin derivatives, esculetin (6,7-dihydroxycoumarin), scoparone (6,7-dimethoxycoumarin), and 4-methylumbelliferone (7-hyroxy-4-methyl) in male Sprague-Dawley rats. Product of lipid peroxidation, malondialdehyde (MDA), activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) were evaluated for oxidative stress in hepatic injury. Gamma glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH) were detected in plasma as a biomarker of hepatic injury. Significantly elevated levels of MDA and lowered levels of SOD and CAT activities were observed in liver of rats exposed to CCl4, when compared to control values. Similarly, administration of CCl4 increased LDH and GGT levels in serum. Pre-treatment of rats with esculetin (35 mg kg(-1), orally) and scoparone (35 mg kg(-1), orally) significantly prevented CCl4-induced decrease in MDA levels and increase in SOD and CAT, whereas 4-methylumbelliferone (35 mg kg(-1)) and coumarin (30 mg kg(-1)) had no effect against CCl4-induced rise in serum enzymes. Esculetin and scoparone also showed protective properties as was evidenced in reduced LDH and GGT levels in serum. The results of this study indicate that the chemical structures of coumarins play an important role in the prevention of oxidative stress.Öğe Influence of coumarin and some coumarin derivatives on serum lipid profiles in carbontetrachloride-exposed rats(Sage Publications Ltd, 2017) Tasdemir, Ezel; Atmaca, Mukadder; Yildirim, Yasar; Bilgin, Hakki Murat; Demirtas, Berjan; Obay, Basra Deniz; Kelle, MustafaIn the present study, coumarin and some coumarin derivatives (esculetin, scoparone, and 4-methylumbelliferone) were investigated for their lipid-lowering effect in rats. Male Sprague-Dawley rats (150-200 g) were divided into six groups and each group comprised of five rats. Hepatic injury-dependent hyperlipidemia was induced by carbon tetrachloride (CCl4, 1.25 ml/kg). Coumarin and coumarin derivatives esculetin (35 mg/kg), scoparone (35 mg/kg), 4-methylumbelliferone (35 mg/kg), or coumarin (30 mg/kg) were administered to experimental groups at 12-h intervals. Animals received the derivatives esculetin, scoparone or 4-methylumbelliferone prior to the administration of a single toxic dose of CCl4. Serum total cholesterol (TC), triglyceride (TG), very low-density lipoprotein cholesterol (VLDL-C), and low-density lipoprotein cholesterol (LDL-C) levels significantly increased in CCl4-treated group (p < 0.05, p < 0.01, p < 0.01, and p < 0.05, respectively), while levels of serum high-density lipoprotein cholesterol (HDL-C) decreased (p < 0.01). 4-Methylumbelliferone had no recovery effects on serum TC levels, however, significantly prevented CCl4-induced hyperlipidemia by reducing TG and VLDL-C levels (p < 0.05 and p < 0.05, respectively). In addition, coumarin had no recovery effect on any of the serum lipid parameters against CCl4-induced hyperlipidemia. Among the coumarin derivatives only esculetin and scoparone significantly prevented serum HDL-C in CCl4-induced dyslipidemia. The results from this study indicate that the chemical structure of coumarins plays an important role on the regulation of serum lipid profiles.Öğe The protective effect of pomegranate extract against cisplatin toxicity in rat liver and kidney tissue(Taylor & Francis Ltd, 2015) Bakir, Salih; Yazgan, Umit Can; Ibiloglu, Ibrahim; Elbey, Bilal; Kizil, Murat; Kelle, MustafaObjectives: The purpose of this study was to perform a histopathological investigation, at the light microscopy level, of the protective effects of pomegranate extract in cisplatin-induced liver and kidney damage in rats. Material and methods: Twenty-eight adult male Wistar albino rats were randomly divided into four groups of seven animals: Group 1: Control; Group 2: Treated for 10 consecutive days by gavage with pomegranate juice (2ml/kg/day); Group 3: Injected intraperitoneally with cisplatin (8mg/kg body weight, single dose) onset of the day 5, and Group 4: Treated by gavage with pomegranate juice 10 days before and after a single injection of cisplatin onset of the day 5. After 10 days, the animals were sacrificed and their kidneys and liver tissue samples were removed from each animal after experimental procedures. Cisplatin-induced renal and hepatic toxicity and the effect of pomegranate juice were evaluated by histopatological examinations. Results: In the kidney tissue, pomegranate juice significantly ameliorated cisplatin-induced structural alterations when compared with the cisplatin alone group. But in the liver tissue, although pomegranate juice attenuated the cisplatin-induced toxicity only in two rats, significant improvement was not observed. Conclusion: In conclusion, these results demonstrate that the anti-oxidant pomegranate juice might have a protective effect against cisplatin-induced toxicity in rat kidney, but not in liver. Pomegranate juice could be beneficial as a dietary supplement in patients receiving chemotherapy medications.Öğe The Protective Effect of Pomegranate Juice against Cisplatin Toxicity in Rat Liver and Kidney Tissue(Wiley-Blackwell, 2015) Bakir, Salih; Yazgan, Umit Can; Ibiloglu, Ibrahim; Elbey, Bilal; Kizil, Murat; Kelle, Mustafa[Abstract Not Available]Öğe Sigara içiminin gastrik miyoelektrik aktiviteye etkisinin araştırılması(Dicle Üniversitesi Tıp Fakültesi, 2021) Kaya, Hamza; Diken, Hüda Oflazoğlu; Kaya, Hacer Kayhan; Kelle, Mustafa; Ekin, Cemre UçarAmaç: Sigara içimi gastrointestinal sistem dahil olmak üzere bir çok doku, organ ve sistemi etkilediği bilinmektedir. Mide motor fonksiyon bozukluklarının bazı belirti ve semptomlarının, elektrogastrografik analiz sonuçlarıyla iyi korelasyon gösterdiği ifade edilmiştir. Şu ana kadar sigaranın mide hareketlerini yöneten gastrik miyoelektrik aktiviteye etkileri konusunda sınırlı sayıda çalışma yapılmıştır. Bu çalışmada, elektrogastrografi (EGG) tekniğinden yararlanılarak sigara içiminin gastrik miyoelektriksel aktiviteye etkisinin incelenmesi amaçlandı. Yöntemler: Çalışma 8’i sigara içen ve 7’si sigara içmeyen 25-40 yaş aralığında ve bilinen gastrointestinal sistem dahil herhangi bir rahatsızlığı bulunmayan toplam 15 sağlıklı ve gönüllü erkek birey üzerinde gerçekleştirildi. Her katılımcı için 30 dakikalık kontrol periyodu ve ardından 60 dakikalık deney periyodu abdominal EGG kaydı alındı. Tüm kayıtlar aç karna, aynı gün içinde ve ardışık olarak kaydedildi. Günde en fazla iki bireyin kaydı yapıldı. EGG kayıtlarındaki normogastri yüzdesi, dominant frekans ve dominant güç parametrelerini gösteren dalgalar; spektral analiz programı kullanılarak analiz edildi. Kontrol periyodunda kronik sigara içiminin, deney periyodunda ise kronik içicilerde akut sigara içiminin etkisi araştırıldı. Çalışmadan elde edilen verilerin değerlendirilmesinde nonparametrik testlerden MannWhitney U testi kullanıldı. Karşılaştırmalardan elde edilen p değerleri p < 0.05 ise anlamlı olarak değerlendirildi. Bulgular: Kontrol periyodunda kronik sigara içen bireylerin EGG kaydı sonuçları sigara içmeyen bireylerin sonuçlarıyla karşılaştırıldığında sigara içenlerde normogastri yüzdesi, dominant frekans ve dominant güç parametrelerinin her üçünde anlamlı olmayan bir azalma saptandı. Ancak, deney periyodunda kronik içicilerde akut sigara içiminin hem normogastri yüzdesini hem de dominant güç değerini anlamlı olarak azalttığı belirlendi. Sonuç: Çalışmadan elde edilen sonuçlar, sigara içiminin özellikle aktif - akut sigara içiminin gastrik miyoelektriksel aktiviteyi azalttığını göstermiştir.Öğe Sigaranın akciğer volüm ve kapasiteleri üzerindeki etkisinin spirometrik metodla araştırılması(2017) Kelle, Mustafa; Denli, M. OrhanBu çalışma, Üniversitemiz personelinden sigara içen ve içmeyen toplam 120 kişi üzerinde yapıldı. Denekler sigara iç me sürelerine ve biyometrik özelliklerine göre 3 grup halinde incelendi. Tüm bireylerin spirometrik parametreleri GODART EXPIR0GRAPH cihazı ile ayrı ayrı ölçüldü. Sigara içen ve aynı biyometrik özelliklere sahip siga ra içmeyen kişilerin saptanan spirometrik parametreleri karşı laştırıldı. 10 yıldan az süreyle sigara içenlerin MSK, FRK,IK, 10-20 yıl süreyle sigara içenlerin PRK, ZVK, ZVK /VK, RV, RV/TAK ve 20 yıldan daha fazla süreyle sigara içenlerin ise 2RK, MSK, ZVK, ZVK^VK, RV, RV/TAK değerlerinde önemli deği şiklikler saptandı. Sonuç olarak; sigaranın solunum fonksiyonu üzerinde ö- nemli etkileri olduğu ve spirometrik parametrelerde meydana gelen değişikliklerin sigara içme süresiyle orantılı bir şe kilde arttığı tespit edildi.Öğe Time-dependent changes in changes in plasma ghrelin and angiotensin II levels in the diabetic nephropathy model(Parlar Scientific Publications, 2021) Gül, Cihan; Kelle, Mustafa; Baylan, Mukadder; Yokuş, Beran; Taşdemir, EzelGhrelin is an appetite-enhancing anabolic hormone secreted from the stomach. Angiotensin II maintains sodium and potassium levels in body fluids and plays a very important role in the regulation of arterial blood pressure. Although their relationship with Type 2 diabetes and complications have been reported, their role in diabetic nephropathy is not fully understood. We investigated time dependent possible changes in plasma ghrelin and angiotensin II levels during the development and progression of diabetic nephropathy in experimental diabetic rat model. Adult 63 male Wistar Albino rats were randomly divided into 9 groups as 4 control (C1-C4), 4 diabetic (D1-D4) and one treatment (T) group. Group D1, sacrificed by cardiac puncture one week after diabetes, group D2 three weeks later, group D3 six weeks later, and groups D4 and T eight weeks later. Antidiabetic treatment was not administered to the D1-D4 group diabetic rats. Group T diabetic rats were treated with antidiabetic metformin (100 mg / kg / day) for 8 weeks. A single dose of 35 mg / kg intraperitoneal streptozotocin was administered to the rats to induce diabetes. Significant differences were found between the D4 and C4 groups in body weight, plasma glucose, ghrelin and angiotensin II, serum and urine creatinine levels. While there was a linear (positive) relationship between plasma ghrelin levels of all rats and urinary creatinine and creatinine clearance and body weight, negative correlations were found between plasma ghrelin and angiotensin II levels and fasting blood glucose levels of all rats. During the progression of diabetes and the development of diabetes-related nephropathy, plasma angiotensin and serum creatinine levels increased, while plasma ghrelin levels decreased over time. Therefore, it was concluded that changes in plasma ghrelin and angiotensin II levels in diabetic rats may be associated with the pathogenesis of diabetic nephropathyÖğe TIME-DEPENDENT CHANGES IN PLASMA GHRELIN AND ANGIOTENSIN II LEVELS IN THE DIABETIC NEPHROPATHY MODEL(Parlar Scientific Publications (P S P), 2021) Gul, Cihan; Kelle, Mustafa; Baylan, Mukadder; Yokus, Beran; Tasdemir, EzelGhrelin is an appetite-enhancing anabolic hormone secreted from the stomach. Angiotensin II maintains sodium and potassium levels in body fluids and plays a very important role in the regulation of arterial blood pressure. Although their relationship with Type 2 diabetes and complications have been reported, their role in diabetic nephropathy is not fully understood. We investigated time dependent possible changes in plasma ghrelin and angiotensin II levels during the development and progression of diabetic nephropathy in experimental diabetic rat model. Adult 63 male Wistar Albino rats were randomly divided into 9 groups as 4 control (C1-C4), 4 diabetic (D1-D4) and one treatment (T) group. Group D1, sacrificed by cardiac puncture one week after diabetes, group D2 three weeks later, group D3 six weeks later, and groups D4 and T eight weeks later. Antidiabetic treatment was not administered to the D1-D4 group diabetic rats. Group T diabetic rats were treated with antidiabetic metformin (100 mg / kg / day) for 8 weeks. A single dose of 35 mg / kg intraperitoneal streptozotocin was administered to the rats to induce diabetes. Significant differences were found between the D4 and C4 groups in body weight, plasma glucose, ghrelin and angiotensin II, serum and urine creatinine levels. While there was a linear (positive) relationship between plasma ghrelin levels of all rats and urinary creatinine and creatinine clearance and body weight, negative correlations were found between plasma ghrelin and angiotensin II levels and fasting blood glucose levels of all rats. During the progression of diabetes and the development of diabetes-related nephropathy, plasma angiotensin and serum creatinine levels increased, while plasma ghrelin levels decreased over time. Therefore, it was concluded that changes in plasma ghrelin and angiotensin II levels in diabetic rats may be associated with the pathogenesis of diabetic nephropathy.
