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Öğe Development of molecular imprinting-based smart cryogels for selective recognition and separation of serum cytochrome-c as a biochemical indicator(Elsevier Sci Ltd, 2021) Canpolat, Gurbet; Dolak, Ibrahim; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, ArzuThe recognition and detection of proteins has an important role in the fields of biochemistry and medicine in term of diagnosis and prognosis. In this study, molecularly imprinted cryogel (MIP) was synthesized for the selective recognition of Cytochrome-c (Cyt-c) by cryopolymerization techniques with the lanthanide-chelate approach using (MAAP)2-Ce(III) as complex functional monomer and Cyt-c as a template protein. The incorporation of template with monomer was characterized by near- infrared (Near-IR) spectroscopy. The binding capacity was optimized in accordance with numerous experimental parameters including pH, initial Cyt-c concentration, flow rate, temperature and ionic strength. As a result, the maximum binding capacity reached to 98.33 mg g-1 in a buffer system with a pH of 6.0. Beside of the excellent reusability performance of prepared MIP, it was successfully applied to separation of template Cyt-c in the existence of hemoglobin (Hb) and myoglobin (Mb) chosen as interfering proteins. The relative selectivity constants of the Cyt-imprinted polymer were determined as 13.84 for the Cyt/Mb pair and 16.05 for the Cyt/Hb pair. Consequently, a high selectivity, simple and cheap strategy for efficient separation of proteins was provided with this novel MIP cryogel.Öğe Introduction to Green Sample Preparation(Royal Soc Chemistry, 2023) Canpolat, Gurbet; Dolak, Ibrahim; Hussain, Chaudhery Ghazanfar; Kecili, Rustem; Hussain, Chaudhery MustansarÖğe Ion imprinted cryogel-based supermacroporous traps for selective separation of cerium(III) in real samples(Elsevier, 2018) Kecili, Rustem; Dolak, Ibrahim; Ziyadanogullari, Berrin; Ersoz, Arzu; Say, RidvanThis study demonstrates the preparation and characterization of a novel ion imprinted cryogel which exhibits high affinity and selectivity towards Ce(III) ions in aqueous solutions and bastnasite ore samples. 2-Hydroxyethyl methacrylate (HEMA) and N-methacryloylamido antipyrine (MAAP) were used as functional monomers for the preparation of Ce(III) imprinted cryogel. The effects of various factors such as initial Ce(III) concentration, flow rate, pH, interaction time and ionic strength on the Ce(III) binding to the prepared ion imprinted cryogels were also studied. The binding equilibrium for Ce(III) is obtained in 30 min at the flow rate of 0.5 mL/min. The maximum binding capacity of the prepared ion imprinted cryogel towards Ce(III) is obtained as 36.58 mg/g at optimum conditions. The selectivity of the prepared ion imprinted cryogel towards Ce(III) in the presence of other possible interfering lanthanide ions such as La(III) and Nd(III) were also performed. The obtained results showed that the prepared ion imprinted cryogel exhibits high selectivity and sensitivity towards Ce(III) ions. The limit of detection (LOD) was found as 50 mu g/L. (C) 2018 Published by Elsevier B.V. on behalf of Chinese Society of Rare Earths.Öğe Ion-Imprinted Polymers for Selective Recognition of Neodymium(III) in Environmental Samples(Amer Chemical Soc, 2015) Dolak, Ibrahim; Kecili, Rustem; Hur, Deniz; Ersoz, Arzu; Say, RidvanThis study describes the selective recognition of Nd(III) in aqueous, solutions in the presence of various lanthanide ions by molecularly imprinted polymers. Molecularly imprinted polymers (MIPs) were prepared by suspension polymerization. N-methacryloylamido folic acid (MAFol) was used as the functional monomer. The effects of different variables such as pH, time, and initial Nd(III) concentration on binding characteristics were investigated. Binding, equilibrium time was achieved in 30 min. The maximum binding capacity was found to be 14.6 mg Nd(III) g(-1) polymer. Binding studies of W(III) in the presence of Ce(III), La(III), and Eu(III),ions were also carried out using Nd(III)-imprinted polymers. The imprinted polymers displayed high selectivity toward Nd(III) ions. The obtained binding order under competitive conditions was Nd(III) > La(III) > Ce(III) > Eu(III).Öğe Molecularly imprinted affinity cryogels for the selective recognition of myoglobin in blood serum(Elsevier Science Bv, 2018) Dolak, Ibrahim; Kecili, Rustem; Onat, Ruken; Ziyadanogullari, Berrin; Ersoz, Arzu; Say, RidvanThe present work demonstrates the selective recognition of myoglobin in human serum in the presence of various competitive proteins such as lysozyme, cytochrome c and hemoglobin by using molecularly imprinted affinity cryogels. The imprinted affinity cryogels towards myoglobin were prepared by using cryopolymerization technique. For this purpose, N-methacryloylamido antipyrine (MAAP)-Ce(III) and myoglobin were used as the complex functional monomer and template molecule, respectively. (MAAP)-Ce(III) complex functional in the imprinted cryogel provided a luminescence feature to the cryogel and the detection of the myoglobin was successfully achieved by UV-VIS-Near Infrared (UV-VIS-NIR) Spectroscopy. The effects of some variables such as initial myoglobin concentration, temperature, pH and ionic strength on myoglobin binding to the prepared cryogels were studied. The myoglobin binding capacity of the prepared cryogels was determined to be 68 mgg(-1). Myoglobin binding to the cryogels in the presence of competitive proteins lysozyme, cytochrome c and hemoglobin were also performed. The imprinted cryogels exhibited high selectivity towards myoglobin and the obtained binding order under competitive conditions was myoglobin > lysozyme > cytochrome c > hemoglobin. (C) 2018 Elsevier B.V. All rights reserved.Öğe A novel lanthanide-chelate based molecularly imprinted cryogel for purification of hemoglobin from blood serum: An alternative method for thalassemia diagnosis(Elsevier Sci Ltd, 2020) Dolak, Ibrahim; Canpolat, Gurbet; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, ArzuPurification and analyzing of proteins is an essential means for understanding their function and diseases associated with their lack or defect. In this research, a new lanthanide-chelate based molecularly imprinted polymer (MIP) was synthesized for selective separation of Hemoglobin (Hb) from human serum in the presence of various interference molecules. The Hb-imprinted polymer was prepared by using complex functional monomer N-methacryloylamido antipyrine (MAAP)-Ce(III) and 2-Hydroxyethyl methacrylate (HEMA) in accordance of cryopolymerization techniques. The nonimprinted cryogel (NIP) was also prepared at same polymerization conditions in the absence of template Hb molecule. The effects of pH, initial Hb concentration, flow rate, temperature and ionic strength on the binding capacity of both imprinted and nonimprinted cryogels was investigated. The maximum binding capacity for the MIP column was found to be as 79.41 mg g(-1) dry cryogel, that is four times higher than the NIP column under the optimum conditions (pH 5.0, flow rate: 1.0 mL min(-1), T: 25 degrees C). Moreover, selectivity experiments were performed by using two interference proteins as myoglobin (Mb) and cytochrome c (Cyt-c) and the relative selectivity coefficients (k') for Hb/Mb and Hb/Cyt-c pairs were determined as 36.59 and 37.22, respectively.Öğe The use of magnetic nanoparticles in sample preparation devices and tools(Elsevier, 2020) Kecili, Rustem; Buyuktiryali, Sibel; Dolak, Ibrahim; Hussain, Chaudhery Mustansar[Abstract Not Available]
