Yazar "Kayis, Seyit Ali" seçeneğine göre listele

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    Expression of Epidermal Growth Factor (EGF) and Heparin-Binding EGF (HB-EGF) mRNA in Mare Endometrium
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Atli, Mehmet Osman; Guzeloglu, Aydin; Kurar, Ercan; Kayis, Seyit Ali; Aslan, Selim; Semacan, Ahmet; Celik, Sefa
    EGF and HB-EGF play crucial roles in embryonic development and peri-implantation. In this study, aim was to characterize expression profiles of EGF and HB-EGF in mare endometrium during estrous cycle and early pregnancy. Endometrium biopsies were obtained from mares on day of ovulation (d0), late diestrus (LD) and after luteolysis (AL) in the estrus phase. In pregnant groups, biopsies were taken on days 14 (P14), 18 (P18), 22 (P22) and 60 (P60). Relative expression levels of genes were quantified using real-time RT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significantly different group(s). EGF mRNA expression was up-regulated at LD compared to d0 while HB-EGF expression was not changed throughout the cycle. EGF expression was also increased during early pregnancy with the highest expression level observed on P60. Similarly, HB-EGF mRNA level was increased on P60. Pregnancy induced EGF expression on P14 and P18 compared to LD and AL whereas expression of HB-EGF was only significantly higher on P18 than that of AL. These results indicate that EGF expression is up-regulated during the cycle at late diestrus when P4 is high and is increased by pregnancy. HB-EGF expression is induced later in the pregnancy. In conclusion, EGF and HB-EGF appear to involve in the events that happen in the mare endometrium during peri-implantation period.
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    Expression of platelet activating factors and its receptor in cat uterus during early pregnancy
    (Ankara Univ Press, 2013) Agaoglu, Ali Reha; Agaoglu, Ozgecan Korkmaz; Kurar, Ercan; Guzeloglu, Aydin; Kayis, Seyit Ali; Atli, Mehmet Osman; Schafer-Somi, Sabine
    Platelet activating factors (PAF1 beta, PAF1 gamma, PAF:ah, PAF-R) have a significant role during mammalian pregnancy process. This study aimed to investigate expression profiles of PAF gene family in cat uterus during pregnancy. Tissue samples were collected via ovariohysterectomy at days 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25 (Gr3; n=5) after mating from the different regions of the uterus. Relative expression levels of genes were quantified using real-time qRT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significant differences between groups. Expressions of PAP genes were assessed in the cat uterus during pregnancy around implantation period; however, no significant differences were detected between the preimplantation and the implantation stage. Similarly, no significant difference concerning mRNA expression was assessed between different parts of the uterus during pregnancy In conclusion, PAP genes are expressed in the cat uterus during early pregnancy. However, this expression is not associated with changes in the uterus during early pregnancy.