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Öğe Circulating miRNAs as potential molecular biomarker of early pregnancy in ewes(Wiley, 2018) Kose, M.; Kaya, M. S.; Kirbas, M.; Dursun, S.; Hitit, M.; Alak, I.; Atli, M. O.[Abstract Not Available]Öğe Early and efficient pregnancy diagnosis in ewes: expression profile of ISG15 mRNA in peripheral blood leucocytes compared to transrectal ultrasonography(Indian Counc Agricultural Res, 2017) Kiyma, Z.; Kose, M.; Kaya, M. S.; Atli, M. O.The objective of this study was to compare the measurement of one of interferon-tau stimulated genes (ISGs; ISG15) expression in peripheral blood leukocytes (PBLs) with transrectal ultrasonography (TUSG) for early pregnancy detection in ewes. Ewes (46) were examined 18 and 25 days after mating by TUSG. PBL samples were collected from 16 pregnant ewes on the day of mating (d0) and on days 15 and 18 after mating. Total RNAs were extracted from PBLs and converted to cDNA. ISG15 mRNA expression was detected using qPCR in triplicate. All pregnancies were confirmed by birth. In TUSG examinations on day 18, the embryonic vesicle and embryonic vesicle plus the embryo were visible for 14 and 32 ewes, respectively, of the 46 total. Both the embryonic vesicle and embryo were detected in 42 ewes on day 25. Four ewes out of 46 with a visible embryonic vesicle on day 18 were detected as non-pregnant on day 25. While significant differences in ISG15 mRNA expression levels of PBLs were detected in 13 of 16 ewes on day 15, ISG15 mRNA levels for all ewes were significantly different on day 18, compared with levels on the day of mating. The results suggested that profiles of ISG15 mRNA can provide pregnancy detection earlier than TUSG.Öğe Early pregnancy regulates genes of hyaluronan system in ovine endometrium(Wiley-Blackwell, 2016) Kiyma, Z.; Hitit, M.; Kose, M.; Kaya, M. S.; Kirbas, M.; Dursun, S.; Atli, M. O.[Abstract Not Available]Öğe Early pregnancy regulates Toll-like receptors at mRNA level in ovine peripheral blood leucocytes(Wiley-Blackwell, 2014) Kaya, M. S.; Kose, M.; Aydilek, N.; Karahan, S.; Kiyma, Z.; Guzeloglu, A.; Atli, M. O.[Abstract Not Available]Öğe Effect of dietary restriction on sperm characteristic and oxidative status on testicular tissue in young rats exposed to long-term heat stress(Wiley, 2015) Aydilek, N.; Varisli, O.; Kocyigit, A.; Taskin, A.; Kaya, M. S.This study was conducted to evaluate the effects of dietary restriction on oxidative status and sperm parameters in rats exposed to long-term heat stress. Forty healthy Sprague-Dawley rats, aged 2.5month, were divided into four groups of 10 with respect to feeding and temperature regimen (room temperature (22 degrees C)-ad libitum, room temperature-dietary restriction (40%), high temperature (38 degrees C)-ad libitum, high temperature-dietary restriction). At the end of the 9th week, some oxidants (lipid hydroperoxide, total oxidant status, oxidative stress index) and antioxidants (total antioxidant status, sulfhydryl groups, ceruloplasmin, paraoxonase and arylesterase activities) were measured in the testis tissue. The concentration, motility, volume, abnormal sperm count, acrosome and membrane integrity of epididymal spermatozoon and intratesticular testosterone levels were evaluated. High temperature did not change oxidative and antioxidative parameters except for sulfhydryl groups and ceruloplasmin, yet it impaired all sperm values. Neither sperm values nor oxidative status apart from sulfhydryl groups, ceruloplasmin and arylesterase was affected by dietary restriction in the testis tissue. These results suggest that long-term heat stress does not have a significant effect on testicular oxidative status, while the spermatozoa are sensitive to heat stress in young rats. Dietary restriction failed to improve the sperm quality and oxidative status except some individual antioxidant parameters; conversely, it decreased intratesticular testosterone level in the young rats exposed to long-term heat stress.Öğe Expression and localization of Toll-like receptor 4 in ovine endometrial and trophoblastic tissues at early pregnancy(Wiley-Blackwell, 2015) Atli, M. O.; Kiyma, Z.; Kose, M.; Kaya, M.; Kaya, M. S.; Yaman, T.; Guzeloglu, A.[Abstract Not Available]Öğe Expression of interferon stimulated genes (ISGs) is extended to pituitary in early pregnant ewes(Wiley-Blackwell, 2016) Ozel, C.; Kose, M. O.; Atli, M.; Hitit, M.; Kiyma, Z.; Kaya, M. S.; Sen, G.[Abstract Not Available]Öğe Expression pattern of TLR4 mRNA and protein in the ovine corpus luteum during the early pregnancy and PGF2? induced luteolysis(Wiley, 2017) Atli, M. O.; Kose, M.; Hitit, M.; Kaya, M. S.; Bozkaya, F.; Guzeloglu, A.[Abstract Not Available]Öğe Expression patterns of TLR2 and TLR4 in the bovine corpus luteum during the different stages of the estrous cycle(Wiley-Blackwell, 2015) Kose, M.; Kaya, M. S.; Aydilek, N.; Guzeloglu, A.; Wiltbank, M. C.; Atli, M. O.[Abstract Not Available]Öğe Expression profile of interferon tau-stimulated genes in ovine peripheral blood leukocytes during embryonic death(Elsevier Science Inc, 2016) Kose, M.; Kaya, M. S.; Aydilek, N.; Kucukaslan, I.; Bayril, T.; Bademkiran, S.; Kiyma, Z.Early and efficient detection of embryonic death (ED) has a valuable impact as important as early pregnancy diagnosis in ruminants. Among early pregnancy diagnosis methods, detection of the expression of interferon tau stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) is well documented in cows and ewes. Therefore, we hypothesized that the expression profile of ISGs in PBLs might also be useful for detecting ED in these animals. For this purpose, pregnant ewes were used as an experimental model. Pregnancy was detected on Day 18 after mating by transrectal ultrasonography. Pregnant ewes were divided into a control group (sham injection on Day 18, n = 10) and ED group (treated with 75 mu g synthetic PGF(2 alpha) on Day 18, n = 12). PBLs and plasma were collected on Days 0 (mating day), 15, 18, 19, 20, 21, 23, and 25 by jugular venipuncture. Total RNA was isolated from PBLs. ISGs expression levels were determined by real-time polymerase chain reaction in triplicate. Electrochemiluminescence immunoassay was used to measure progesterone (P-4) levels in plasma. In the ED group, the P-4 level declined to less than 1 ng/mL on Day 19 and remained at a low level until the end of the study. Compared with that on Day 0, receptor transporter protein 4 (RTP4) and ISG15 expression was upregulated on Day 15 and remained high until Day 21 in both groups, and RTP4 and ISG15 mRNA levels were attenuated on Days 23 and 25 only in the ED group (P < 0.001). Myxovirus resistance 1 expression was upregulated on Day 15 and remained high until Day 23 in both groups, but was attenuated on Day 25 in the ED group (P < 0.05). The B2-microglobulin mRNA level did not change significantly during the study in either group. These results indicate that the decline in P-4 concentration was an immediate response to PGF(2 alpha) and that the embryo may have survived longer than the CL on the basis of the extended period of ISGs expression. This suggests that the absence of P-4 could be the reason for ED rather than a direct effect of PGF(2 alpha). In conclusion, the expression of ISGs, including ISG15, RTP4, and myxovirus resistance 1, but not B2-microglobulin, in PBLs may serve as a marker of ED. (C) 2016 Elsevier Inc. All rights reserved.Öğe Expression profile of receptor transporter protein 4 (Rtp4) mRNA in ovine peripheral blood leucocytes (PBLs) during early embryonic death (EED)(Wiley-Blackwell, 2014) Kose, M.; Kaya, M. S.; Aydilek, N.; Kucukaslan, I.; Bayril, T.; Bademkiran, S.; Kiyma, Z.[Abstract Not Available]Öğe Expression profile of Toll-like receptor 7 in the ovine corpus luteum during prostaglandin F2?-induced luteolysis(Wiley, 2018) Atli, M. O.; Kose, M.; Hitit, M.; Kaya, M. S.; Bozkaya, F.[Abstract Not Available]Öğe Expression profiles of critical miRNAs in ovine endometrium during the peri-implantation(Wiley, 2018) Dursun, S.; Kose, M.; Kaya, M. S.; Kirbas, M.; Hitit, M.; Alak, I.; Atli, M. O.[Abstract Not Available]Öğe miRNAs expression during the peri-attachment period of pregnancy in the ovine endometrium(Wiley-Blackwell, 2016) Kose, M.; Ozel, C.; Guzeloglu, A.; Kaya, M. S.; Kirbas, M.; Dursun, S.; Kiyma, Z.[Abstract Not Available]Öğe Profile of Pregnancy Associated Glycoproteins in bovine plasma during postpartum period; is it usable for detecting pregnancy loss?(Wiley-Blackwell, 2015) Kaya, M. S.; Kose, M.; Coban, S.; Mutlu, H.; Ucar, E. H.; Guzeloglu, A.; Atli, M. O.[Abstract Not Available]
