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Öğe Automatic Identification of Adenoid Hypertrophy via Ensemble Deep Learning Models Employing X-ray Adenoid Images(Springer, 2025) Orenc, Sedat; Acar, Emrullah; Ozerdem, Mehmet Sirac; Sahin, Sefer; Kaya, AbdullahAdenoid hypertrophy, characterized by the abnormal enlargement of adenoid tissue, is a condition that can cause significant breathing and sleep disturbances, particularly in children. Accurate diagnosis of adenoid hypertrophy is critical, yet traditional methods, such as imaging and manual interpretation, are prone to errors. This study uses an ensemble deep learning-based approach for adenoid classification. It utilizes a unique dataset sourced from Batman Training and Research Hospital. The dataset is composed of masked and non-masked X-ray images. It is used to train and compare the performance of multiple convolutional neural network (CNN) models. By comparing classification accuracy between masked and non-masked datasets, the study reveals the importance of image preprocessing. Six deep learning models-EfficientNet, MobileNet, ResNet50, ResNet152, VGG16, and Xception-are tested, with ResNet50 achieving the highest accuracy (100% on masked images), while Xception performs the worst (65% F1-score). The results indicate that masking significantly enhances the accuracy and reliability of adenoid classification. ResNet50 and EfficientNet show strong generalization capabilities. Conversely, the lower performance of models like Xception highlights the variability in model suitability for this task. This research provides valuable insights into optimizing deep learning models for medical image classification and it advances the field of AI-based adenoid detection.Öğe Comparison of tuberculin skin test, IFN-? assay, real time PCR and lateral flow rapid test in diagnosis of field outbreaks of bovine tuberculosis(Kafkas Üniversitesi Veteriner Fakültesi, 2015) Kaya, Abdullah; İçen, Hasan; Tuzcu, Nevin; Şimşek, Aynur; Yeşilmen, Simten; Koçhan, Akın; Yıldırım, İbrahim HalilBovine tuberculosis is an important zoonotic disease transmitted by direct contact, respiratory pathway, ingestion of unpasteurised milk and milk product, raw or undercooked meat. Tuberculosis can be difficult to diagnose based only on the clinical signs. Tuberculosis is usually diagnosed in the field with the tuberculin skin test. Sputum and other body fluids may be collected for microbiological examination. Polymerase chain reaction (PCR) methods have also been described. Diagnostic blood tests include the lymphocyte proliferation assay, the interferon gamma (IFN-γ) assay, and enzyme-linked immunosorbent assays (ELISA). In this study a total of 50 animals were tested by using tuberculin skin test (TST), lateral flow rapid test, IFN-γ assay and real time PCR. The animals were selected randomly among 178 cattle in dairy farms with the aged between 3-5 years and suspected of having tuberculosis. Forty five cattle were positive out of 50 for TST while 31 for reactive by the IFN-γ assay and 28 for rapid test and 9 for real time PCR. The purpose behind such variable as age was to compare sensitivity of tuberculin skin test, the IFN-γ assay and TB lateral flow rapid test and real time PCR examination for the diagnosis of field outbreaks of bovine tuberculosis in Turkey.Öğe Dynamics of microRNAs in bull spermatozoa(Biomed Central Ltd, 2012) Govindaraju, Aruna; Uzun, Alper; Robertson, LaShonda; Atli, Mehmet O.; Kaya, Abdullah; Topper, Einko; Crate, Elizabeth A.Background: MicroRNAs are small non-coding RNAs that regulate gene expression and thus play important roles in mammalian development. However, the comprehensive lists of microRNAs, as well as, molecular mechanisms by which microRNAs regulate gene expression during gamete and embryo development are poorly defined. The objectives of this study were to determine microRNAs in bull sperm and predict their functions. Methods: To accomplish our objectives we isolated miRNAs from sperm of high and low fertility bulls, conducted microRNA microarray experiments and validated expression of a panel of microRNAs using real time RT-PCR. Bioinformatic approaches were carried out to identify regulated targets. Results: We demonstrated that an abundance of microRNAs were present in bovine spermatozoa, however, only seven were differentially expressed; hsa-aga-3155, -8197, -6727, -11796, -14189, -6125, -13659. The abundance of miRNAs in the spermatozoa and the differential expression in sperm from high vs. low fertility bulls suggests that the miRNAs possibly play important functions in the regulating mechanisms of bovine spermatozoa. Conclusion: Identification of specific microRNAs expressed in spermatozoa of bulls with different fertility phenotypes will help better understand mammalian gametogenesis and early development.Öğe Investigation of Protective and Therapeutic Efficacy of Lactoferrin on Neonatal Calf Diarrhea(Dicle University, 2024) Işık, Nurettin; Kaya, Abdullah; İçen, HasanThe aim of this study was to investigate the prevalence of rotavirus, coronavirus, Cryptosporidium, E. coli F5 (K99), Cl. perfringens and Salmonella spp. and clinical, haematological and biochemical changes in calves with neonatal diarrhoea and the efficacy of lactoferrin supplementation in standard treatment in Van and Diyarbakır provinces. The calves with diarrhea were investigated by immunochromatographic test kit and conventional bacteriological methods. Rotavirus was detected in 51%, Cryptosporidium in 35%, Cl. perfringens in 23%, coronavirus in 16%, E. coli F5 (K99) in 4%, Salmonella spp. in 2% of the calves with diarrhea. Giardia spp. was not detected in any calf, while 65% of the calves had a single agent, 33% had more than one agent. In vitro bactericidal and bacteriostatic effects of lactoferrin on E. coli O157, E. coli F5 and Salmonella enteriditis were investigated. Lactoferrin was found to be effective on bacteria at concentrations of 100 mg/ml and higher, decreased the mortality rate and showed a positive effect on clinical parameters. It was concluded that lactoferrin can be used for preventive and therapeutic purposes at doses of 100 mg/ml and higher and will be more effective in treatment.Öğe Prevalence of Four Enteropathogens with Immunochromatographic Rapid Test in the Feces of Diarrheic Calves in East and Southeast of Turkey(Univ Agriculture, Fac Veterinary Science, 2013) Icen, Hasan; Arserim, Neval Berrin; Isik, Nurettin; Ozkan, Cumali; Kaya, AbdullahIn this study, fecal specimens taken from 192 diarrheic and 14 healthy calves (2-40 days old) were examined for the presence of bacterial and parasitic agents. Fecal samples from diarrheic calves with the four immunochromatographic rapid tests were 92.7% positive for four enteropathogens. The individual prevalence was 25, 21.8, 9.4 and 2.1% for Rotavirus, Cryptosporidium parvum, E. coli K99 and Coronavirus, respectively. Concomitant infections caused by two agents were 15.6% for Rotavirus+Cryptosporidium, 1.0% for Rotavirus+Coronavirus, 5.2 % for Cryptosporidium+E. coli K99, and 7.3% for Rotavirus+E. coli K99. Besides concomitant infections caused by three agents were 3.1% for Cryptosporidium +Rotavirus+E. coli K99 and 1.0%, Cryptosporidium+Rotavirus+Coronavirus. In addition one calf (1.0%) was infected by combination of four agents as Cryptosporidium, Rotavirus, Coronavirus, and E. coli K99. The calculated individual prevalence was 56.9% for Rotavirus, 47.8% for C. parvum, 26.0% for E. coli K99 and 5.2% for Coronavirus. However, 88 samples were positive in smear detection for Cryptosporidium while 92 were positive in rapid test. As a result of this study it can be concluded that multiple etiologies of diarrhea can be seen and this can help in the development of a specific treatment and preventative measures for practitioners in east and southeast of Turkey. (C) 2013 PVJ. All rights reservedÖğe Van ili ve çevresinde 2000-2003 yılları arasında görülen hastalıkların insidansı ve mevsimlere göre dağılımı üzerine araştırmalar(2007) Şimşek, Aynur; Kaya, AbdullahBu çalısmanın amacı; Van ili ve çevresinde 2000-2003 yılları arasında görülen hayvan hastalıkları profilinin arastırılmasıdır. Çalısmanın materyali; bölgede hayvancılık ile ilgili faaliyet gösteren resmi ve özel kuruluslar tarafından tutulan kayıtlardır. Çalısmada 2000 yılında 998, 2001 yılında 724, 2002 yılında 430, 2003 yılında 868 ve toplam 3020 olgu degerlendirildi. Tüm olgular hayvan türü ayrımı yapılmaksızın mevsim, yıl ve sisteme göre sınıflandırıldı. 2000-2003 yılları arasında sindirim sistemi hastalıkları %16.09, dolasım sistemi hastalıkları %1.32, üriner sistem hastalıkları %0.66, solunum sistemi hastalıkları %10.66, sinir sistemi hastalıkları %0.36, deri hastalıkları %0.69, enfeksiyon hastalıkları %16.82, paraziter hastalıklar %13.27, metabolizma ve noksanlık hastalıkları %4.47, zehirlenme olguları %0.39, cerrahi hastalıklar %24.73 ve dogum ve jinekoloji hastalıkları %10.49 oranlarında tespit edildi. Hastalıkların 2000-2003 yılları arasında mevsimsel dagılımları incelendiginde; Hayvanlarda bir hastalıga yakalanma riskinin %37.68 oranıyla en fazla ilkbaharda, %28.11 yaz aylarında, %17.68 kıs mevsiminde ortaya çıktıgı, bu arada hastalıkların görülme oranının en az sonbaharda (%16.52) ortaya çıktıgı anlasılmıstır. Bu nedenle bölgede yapılacak iyilestirme çalısmalarında bu dagılımın göz önünde bulundurulmasının faydalı olacagı kanısına varıldı.