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    THE RELATIONSHIP BETWEEN H. PYLORI AND DIETARY HABITS IN OBESE FEMALE PATIENTS
    (Publ House Bulgarian Acad Sci, 2022) Karaman, Ulku; Kaya, Yasemin; Arici, Yeliz kasko; Arserim, Neval Berrin; Karagoz, Zuhal Karaca; Colak, Cemil; Yolalan, Gamze
    The purpose of this study is to investigate the relationship between the dietary habits of obese women aged above 18 and the Helicobacter pylori (H. pylori). Five hundred and sixty-one women aged above 18 were included in the study. Patients filled in questionnaires consisting of questions regarding their sociodemographic variables, chronic diseases and dietary habits. Blood samples taken from the patients were tested according to the H. pylori kit procedures and the results were categorized as positive and negative. In the study, obese and nonobese patients were compared and then H. Pylori positive and negative groups were compared. No significant difference was found between groups in terms of H. pylori in the comparison of obese and nonobese patients (p = 0.272). When H. pylori positive and negative groups of obese patients were compared in terms of their dietary habits, consumption rates of butter, whole-fat or lowfat milk, cookies-cake etc., and chocolate-candies were detected to be higher in the H. pylori positive group (p < 0.05). Red meat consumption (p = 0.044) and smoking rates were lower (p = 0.001). There was no significant difference between groups in terms of the consumption rates of bread, rice/pasta, corn, cheese, chicken, fish, sausages, fruit, vegetables, legumes and salt. No significant difference was found between obese and nonobese women in terms of H. pylori positivity. It was observed that H. pylori positive obese women had higher consumption rates of butter, whole-fat or low-fat milk, cake-cookies etc., and chocolate-candies.
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    Single-strand conformation polymorphism-based genetic characterization of the Cyclospora cayetanensis strains collected from different provinces in Turkey
    (Inst Agricultural Medicine, 2021) Cicek, Muttalip; Yildirim, Ibrahim Halil; Cengiz, Zeynep Tas; Karaman, Ulku
    Introduction and objective. Cyclospora cayetanensis, a coccidian protozoan species, has been recently found to cause diarrhea in all age groups in immunocompetent and immunocompromised individuals in most regions of the world. This study aimed to conduct the molecular detection of C. cayetanensis and to determine the genetic diversity of the 18S ribosomal RNA (rRNA) gene sequence of C. cayetanensis isolated from individuals living in different provinces in Turkey by using PCR-single-strand conformation polymorphism (SSCP). Materials and method. A total of 22 subjects were included in the study. Fourteen of the subjects were female and eight were male, with ages ranging between 7-65 years. Stool specimens were examined using wet mount and modified acid-fast staining methods, which revealed the presence of oocysts in the samples. The 18S rRNA ITS-1 Ccits37f-GCTTGCTATGTTTTAGCATGTGG and Ccits501r-GCACAATGAATGCACACACA gene regions were used as primers. The PCR products were analyzed by agarose gel electrophoresis and visualized on a UV transilluminator. For the SSCP, the PCR products were denatured with formamide, run for 16 h in 6% (49:1) polyacrylamide gel, and then imaged with silver staining. Results. SSCP assay was performed given that the DNA strands demonstrated different folds; the DNA strands contain different nucleotides based on the PCR-SSCP results for the Cyclospora strains collected in 4 provinces. Moreover, 3 different band profiles were observed in the investigated samples. A slight mutation difference was observed among the strains collected. Conclusions. Further comprehensive studies involving more C. cayetanensis-positive specimens and utilizing different mutation screening methods are warranted to demonstrate mutation differences in Cyclopora strains in Turkey.