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Öğe The effect of UV-B on fatty acid content and radical scavenging activity of methanolic extracts from Hypericum retusum Aucher grown under in vitro conditions(2013) Işkalan Ç.; Karakuş P.; Kuru I.S.; Çelik K.S.The study was aimed to evaluate the effects of UV-B radiation on fatty acid content and antioxidant activity of a methanolic extract of Hypericum retusum Aucher plant-lets grown under in vitro conditions. Therefore, the plant-lets were exposed to UV-B radiation for different durations (T1: 15, T2: 30, T3: 45, T4: 60 min). The UV-B application led to increase in linolenic (42.84%), oleic (6.92%) and linoleic acid (28.48%) in H. retusum. The methanol extracts of plantlets grown in vitro conditions showed the strongest free radical scavenging activities at concentrations of 50, 100, and 300 ng/mL-1. It has been found that UV-B radiation and in vitro growth conditions have a significant effect on the accumulation of fatty acids. However, the free radical scavenging ability of H. retusum extract was decreased after UV-B application.