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Öğe A case of Osteochondroma in a cat's ear(Univ Cordoba, 2024) Polat, Eren; Karabulut, Burak; Ekinci, ElifIn this case report, we aimed to share the clinical and histopathological examination and treatment process of a 6-month-old hybrid cat brought with the complaint of swelling in the medial part of the right auricle. In clinical examination, it was determined that the mass had a hard and irregular surface structure. As a result of the clinical examination, it was decided to completely extirpate the mass and send it to the pathology department. The mass was widely separated from the surrounding tissues and extirpated with the help of cautery. Microscopic examination of the mass revealed that there were chondrocytes among the dense bone cell accumulations and that they formed islands in some areas. As a result of histopathological examinations, it was determined that the suspicious mass was osteochondroma. During the postoperative examinations, it was determined that there was no recurrence or complication in the patient. As a result, it was concluded that this presentation will contribute to the literature since osteochondroma masses are rare in the ear region.Öğe Detection and molecular characterization of Clostridium perfringens, Paeniclostridium sordellii and Clostridium septicum from lambs and goat kids with hemorrhagic abomasitis in Turkey(BioMed Central Ltd., 2023) Kalender, Hakan; Öngör, Hasan; Timurkaan, Necati; Karagülle, Burcu; Karabulut, Burak; İncili, Canan Akdeniz; Ekinci, ElifBackground: The pathogenic Clostridia cause neurotoxic, histotoxic and enterotoxic infections in humans and animals. Several Clostridium species have been associated with abomasitis in ruminants. The present study aimed to investigate the frequency, and the presence of virulence genes, of Clostridium perfringens, Paeniclostridium sordellii and Clostridium septicum in lambs and goat kids with hemorrhagic abomasitis. Results: A total of 38 abomasum samples, collected from lambs and goat kids of 1 week to 1 month of age in different farms located in eastern Turkey between 2021 and 2022, were evaluated by histopathology, culture and PCR. At necropsy, the abomasum of the animals was excessively filled with caseinized content and gas, and the abomasum mucosa was hemorrhagic in varying degrees. In histopathological evaluation, acute necrotizing hemorrhagic inflammation was noted in abomasum samples. The examination of swab samples by culture and PCR revealed that C. perfringens type A was the most frequently detected species (86.84%) either alone or in combination with other Clostridium species. P. sordellii, C. perfringens type F and C. septicum were also harboured in the samples, albeit at low rates. Beta2 toxin gene (cpb2) was found in three of C. perfringens type A positive samples. Conclusion: It was suggested that vaccination of pregnant animals with toxoid vaccines would be beneficial in terms of protecting newborn animals against Clostridial infections. This study investigated the presence of clostridial toxin genes in abomasal samples for the first time in Turkey.Öğe Effects of local application of bovine amniotic fluid on fracture healing in rats (Rattus norvegicus)(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2024) Tanrısever, Murat; İstek, Özmen; Eröksüz, Hatice; Karabulut, Burak; Özcan, Erhan Cahit; Bingül, Bahattin; Güler, Rıdvan; Dündar, SerkanIn this study, it was aim to examine the local application of bovine amniotic fluid on bone fracture healing in rats. Twenty female Sprague dawley rats included in the study were divided into 2 groups of 10. The sham group (n=10): Bone fractures were created in the right tibia bones of the rats and fixed with kirschner wire. After a four–week recovery period, the subjects were sacrificed. Local bovine amniotic fluid group (n=10): Bone fractures were created in the right tibia bones of the rats and local bovine amniotic fuid was applied during fixation with kirschner wire. After a four–week recovery period, the subjects were sacrificed. Samples from all subjects were decalcified, stained with hematoxylin and eosin, and new bone formation and fibrosis were analyzed. When the groups were evaluated in terms of new bone regeneration, it was determined that the new bone regeneration in the subjects treated with local bovine amniotic fluid were statistically significantly higher than sham group (P<0.05). When the groups were evaluated in terms of fibrosis, the fibrosis value in the sham group was found to be statistically significantly higher when compared with the local bovine amniotic fluid group (P<0.05). It can be stated that local bovine amniotic fluid application may positively affect the healing of bone fractures.Öğe Evaluation of the effect of local Bovine Amniotic Fluid on Osseointegration of Titanium Implants: A Histologic and Histomorphometric Study(Univ Zulia, Facultad Ciencias Veterinarias, 2024) Istek, Ozmen; Tanrisever, Murat; Aydin, Melek Atille; Pak, Pinar; Eroksuz, Hatice; Karabulut, Burak; Ekinci, ElifThe aim of this study was to histologically and histomorphometrically investigate the effect of locally applied bovine amniotic fluid (BAF) on osseointegration levels in implants. Adult female Sprague-Dawley rats weighing 300-350 g were used as subjects. The rats were divided into two groups: the sham-operated control group (n=10) and the local BAF group (n=10). Implant cavities were created in the tibias of all subjects under sterile saline cooling with rotating instruments. Local BAF was applied to all implant sockets before the implants were placed. Rats were sacrificed after a four-week osseointegration period. Histological staining was performed using hematoxylin and eosin staining to analyze the osseointegration. Examinations of the bone implant connection (BIC) and peri-implant bone formation (PBF) were performed using a light microscope and an image analyzer. As a result of the analysis, the mean BIC value was 40.3 +/- 4.9 for the sham-operated control group and 45.2 +/- 7.7 for the local BAF group. The mean PBF was 39.9 +/- 6.3 for the sham control group and 40.5 +/- 5.7 for the local BAF group. A statistically significant difference was found between the sham control group and the local BAF group for the BIC and PBF values (P>0.05; P >0.05; P : 0.11; P : 0.83). The application of local BAF to the implant socket did not have a clear positive effect on implant osseointegration. More studies are needed to clarify the association between local BAF and osseointegration.Öğe The histopathological evaluation of effects of application of the bovine amniotic fluid with graft on peri-implant bone regeneration(Kafkas Universitesi Veteriner Fakultesi, 2023) İstek, Özmen; Tanrısever, Murat; Eröksüz, Hatice; Karabulut, Burak; Özcan, Erhan Cahit; Bingül, Muhammet Bahattin; Güler, Rıdvan; Dündar, SerkanThis study aimed to determine the effects of bovine amniotic fluid combined with bone graft in treating peri-implant bone defects with guided bone regeneration. Twenty female Sprague–Dawley rats were divided into two groups. Bone sockets with a diameter of 4 mm in the coronal part and a diameter of 2.5 mm in the apical part of the implant were created into the corticocancellous bone in the metaphyseal parts of the right tibia bones of all subjects. Implants with a length of 4 mm and a diameter of 2.5 mm were placed in the bone sockets. In the sham surgery group (n = 10) was the circumferential bone defect equivalent to half of the 4-mm implant length, which occurred between the implant and the bone, filled with bovine xenograft. Bovine xenografts were filled with amniotic fluid mixture in the experimental group (n = 10). After 8 weeks of recovery, all rats were sacrificed. The implants were extracted from the soft tissues and the surrounding bone. Subsequently, the bones were decalcified and prepared for histological analysis. The percentage of newly regenerated bone (NRB) formation and fibrosis in the bone defect area around the implant was calculated from all sections. NRB was found in 37.4±4.4% of controls and 41.4±2.63% of test animals (P<0.05 and P=0.024, respectively). Fibrosis formation was found at a rate of 38.6±5.06% in the control group and 33.2±5.38% in the test group (P<0.05 and P=0.033, respectively). It was considered that combining bovine amniotic fluid with bone transplant could be a useful way of treating bone abnormalities.
