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Molecular analysis of grapevine germplasm by SSR (Simple Sequence Repeats) in Diyarbakir province, Turkey
(Corvinus University of Budapest, 2019) Karataş, Hüseyin; Karaaǧaç, Eda; Değirmenci, Dilek Karataş; Ağaoğlu, Sabit
This study was aimed to identify the grape cultivars growing in Diyarbakir. A total of 45 genotypes were analyzed using 7 microsatellite loci. The numbers of alleles per locus ranged between 7 and 10, whereby VVS2 and VrZAG47 had the highest and VVMD7, VrZAG62, and VrZAG79 had the lowest number of alleles. The expected and observed heterozygosity were 0.77 and 0.73, respectively. Clustering analysis was performed using the UPGMA method (Unweighted Pair-Group Method using Arithmetic means). A dendrogram was constructed based on the genetic similarity among the genotypes, which indicated 5 distinct groups, with each group involving numerous subgroups. Two additional genotypes that are used as reference genotypes around the world, Cabernet Sauvignon and Merlot, were also analyzed and were classified into a separate subgroup and differentiated from the other 43 genotypes. In conclusion, synonyms and homonyms were detected among some of the genotypes analyzed in the study.