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    Öğe
    The Change in Luteal Blood Flow and Luteal Size after Beta Carotene and GnRH Injections in Early Pregnant Dairy Cows
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Ay, Serhan Serhat; Kucukaslan, Ibrahim; Kaya, Duygu; Mulazimoglu, Serkan Baris; Emre, Birten; Kacar, Cihan; Kalender, Hakan
    The aim of this study was to determine the effectiveness of intramuscular injections of beta-carotene (beta C) and GnRH on luteal size (LS), luteal blood flow (LBF) and serum beta C concentrations in early pregnant cows. Twenty-nine Holstein-Friesian cows with a mature corpus luteum (>19mm) were randomly assigned to two groups: pc not received (beta C-; n=15) or received (beta C-; n=14). All cows were treated with PGF(2)alpha and inseminated twice, 48 and 72h after the treatment. Last Al was considered to be day 0. All cows received GnRH on day inseminations, 7 and 17. Different from the beta C-, the beta C+ group received beta C intramuscularly on day 7 and 17. In both groups, measurement of LS and LBF were performed on days 7, 10, 17,27 and 37 by transrectal B-mode and colour Doppler ultrasonography. Blood samples were collected on each examination day. Only cows that became pregnant were included in the statistical evaluation. The concentration o beta C in the beta C+ group was higher than in the beta C- at all examination days except day 17 (P<0.05). There was no significant difference between groups concerning the progesterone concentrations (P>0.05). The LS and LBF of beta C+ group on day 7 (P<0.05) and 27 (P<0.01) was higher than in the beta C- group and values increased significantly until day 37 (LS: P<0.05, LBF: P<0.01). We conclude that beta C injections significantly increased serum beta C concentrations, as well as LS and LBF.
  • Küçük Resim
    Öğe
    Detection and molecular characterization of Clostridium perfringens, Paeniclostridium sordellii and Clostridium septicum from lambs and goat kids with hemorrhagic abomasitis in Turkey
    (BioMed Central Ltd., 2023) Kalender, Hakan; Öngör, Hasan; Timurkaan, Necati; Karagülle, Burcu; Karabulut, Burak; İncili, Canan Akdeniz; Ekinci, Elif
    Background: The pathogenic Clostridia cause neurotoxic, histotoxic and enterotoxic infections in humans and animals. Several Clostridium species have been associated with abomasitis in ruminants. The present study aimed to investigate the frequency, and the presence of virulence genes, of Clostridium perfringens, Paeniclostridium sordellii and Clostridium septicum in lambs and goat kids with hemorrhagic abomasitis. Results: A total of 38 abomasum samples, collected from lambs and goat kids of 1 week to 1 month of age in different farms located in eastern Turkey between 2021 and 2022, were evaluated by histopathology, culture and PCR. At necropsy, the abomasum of the animals was excessively filled with caseinized content and gas, and the abomasum mucosa was hemorrhagic in varying degrees. In histopathological evaluation, acute necrotizing hemorrhagic inflammation was noted in abomasum samples. The examination of swab samples by culture and PCR revealed that C. perfringens type A was the most frequently detected species (86.84%) either alone or in combination with other Clostridium species. P. sordellii, C. perfringens type F and C. septicum were also harboured in the samples, albeit at low rates. Beta2 toxin gene (cpb2) was found in three of C. perfringens type A positive samples. Conclusion: It was suggested that vaccination of pregnant animals with toxoid vaccines would be beneficial in terms of protecting newborn animals against Clostridial infections. This study investigated the presence of clostridial toxin genes in abomasal samples for the first time in Turkey.