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Öğe The change in luteal blood flow and luteal size after beta carotene and GnRH injections in early pregnant dairy cows(Veteriner Fakultesi Dergisi, 2012) Serhan Serhat A.Y.; Küçükaslan I.; Kaya D.; Mülazimoğlu S.B.; Emre B.; Kaçar C.; Kalender H.The aim of this study was to determine the effectiveness of intramuscular injections of beta-carotene (?C) and GnRH on luteal size (LS), luteal blood flow (LBF) and serum ?C concentrations in early pregnant cows. Twenty-nine Holstein-Friesian cows with a mature corpus luteum (>19mm) were randomly assigned to two groups: ?C not received (?C-; n=15) or received (?C+; n=14). All cows were treated with PGF2? and inseminated twice, 48 and 72h after the treatment. Last AI was considered to be day 0. All cows received GnRH on day inseminations, 7 and 17. Different from the ?C-, the ?C+ group received ?C intramuscularly on day 7 and 17. In both groups, measurement of LS and LBF were performed on days 7, 10, 17, 27 and 37 by transrectal B-mode and colour Doppler ultrasonography. Blood samples were collected on each examination day. Only cows that became pregnant were included in the statistical evaluation. The concentration of ?C in the ?C+ group was higher than in the ?C- at all examination days except day 17 (P<0.05). There was no significant difference between groups concerning the progesterone concentrations (P>0.05). The LS and LBF of ?C+ group on day 7 (P<0.05) and 27 (P<0.01) was higher than in the ?C- group and values increased significantly until day 37 (LS: P<0.05, LBF: P<0.01). We conclude that ?C injections significantly increased serum ?C concentrations, as well as LS and LBF.Öğe The effectiveness of using antibiotic with intravaginal sponge and duration of sponge treatments on the vaginal flora and fertility in anestrous ewes(2008) Özyurtlu N.; Yepilmen S.; Küçükaslan I.The aim of this study was to compare effectiveness of antibiotic administration to intravaginal sponge before sponge insertion and investigate duration of sponge treatment for determining changes in the vaginal bacterial flora and fertility parameters. Intravaginal sponges impregnated with 30 mg FGA were inserted in 30 Awassi ewes for long-term (14 days; LT), long-term with antibiotic added sponges (LT-A) and short-term (7 days; ST) during the non-breeding season. All ewes received 400 IU PMSG at sponge withdrawal. Bacterial counts were performed on the vaginal flora samples obtained before the introduction of the sponges, at sponge withdrawal and day of estrous in the treatment groups. The mean value for the colony forming units (× 10 3 mL-1) were 5.31, 2.92 and 4.91 on the day of intravaginal sponge insertion and increased to 163.97, 68.34 and 147.0 (p<0.05) at sponge withdrawal, decreased on the day of estrous to 6.97, 4.53 and 5.88 in group LT, LT-A and ST, respectively (p<0.05). According to the antibiotics susceptibility test, clindamycin, erythromycin, penicillin and vancomycin were more resistance than the other antibiotics. The frequency of ewes in estrous, pregnancy rates and the interval to onset of estrous were similar among groups in the study (p>0.05). It was concluded that intravaginal sponge treatments increased bacterial counts, but this increase returned normal values at estrous time. Changes in the number of vaginal flora were not different statistically in the antibiotic added and not added sponge treatment groups at sponge withdrawal and estrous time. Antibiotic administrations to sponge prevented bacterial growth by fist days of sponge treatment. However, this did not affect bacterial count and reproductive response on the day of estrous. © Medwell Journals, 2008.