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    Antiproliferative effect of Potentilla fulgens on glioblastoma cancer cells through downregulation of Akt/mTOR signaling pathway
    (Wolters Kluwer Medknow Publications, 2023) Kandemir, Sevgi Irtegun; Ipek, Polat
    Background: Glioblastoma multiforme (GBM) is the most aggressive brain tumor that is common among adults. This aggression is due to increased invasion, migration, proliferation, angiogenesis, and decreased apoptosis. Plant-based compounds have a high potential to be used as an anticancer agent due to their various mechanisms and less undesirable side effects. Potentilla fulgens is a medicinal plant, and methanolic root extract of P. fulgens (PRE) has anti-inflammatory and anticancer properties.Objective: In this study, we aimed to investigate antiproliferative effect of PRE on U118 and T98G glioblastoma cancer cells and to reveal which molecular signaling pathways regulate this mechanism of action.Materials and Methods: The effect of PRE on cell viability of GBM cells was investigated by MTT assay. Involvement of PRE with cell growth and survival signaling pathways, phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR and c-Src/signal transducer and activator of transcription 3 (STAT3), was examined using Western Blot.Results: PRE reduced cell viability of GBM and human dermal fibroblast (HDF) cells in a dose-and time-independent manner. PI3K expression/phosphorylation level remained unchanged in both GBM and HDF cells after PRE treatment, but Akt/mTOR signaling pathway was downregulated in PRE-treated cells. PRE treatment did not affect c-Src expression/phosphorylation level in GBM cells; however, expression of c-Src was suppressed in HDF cells. Similar results were observed for STAT3 expression and phosphorylation status.Conclusion: PRE has the ability to suppress cell viability in GBM cells, by targeting the Akt/mTOR signaling pathway.
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    The Effects of Dietary Wheat Straw, Red Lentil Straw and Equal Mixtures of Straws as ad libitum on Intensive Fattening Performance, Some Serum Minerals and Economic Efficiency in Awassi Lambs
    (Medwell Online, 2012) Demirel, Ramazan; Ipek, Polat; Tatar, Ali Murat; Demirel, Dilek Senturk
    The objective of this study was to compare the effects of wheat and red lentil (Lens esculenta) straw on the growth performance, feed intake and its efficiency, serum minerals and economic efficiency in Awassi lambs. The project was carried out in the birth season of 2010 in the experimental unit of sanliurfa GAP Agricultural Research Institute with 30 male fat-tailed Awassi breed lambs in 3 groups after weaning period (approximately 3 months of age). Animals were grouped based on their initial live weights. Lambs, straws, concentrate lamb feed and remaining of both feeds were weighed for 14 days intervals. At the beginning, middle and end of the trial, blood samples were collected. Thirty Awassi lambs were randomly assigned to three experimental groups with three diets as follows; D1: Wheat Straw (WS-ad libitum) + Lamb Mixed Feed (LMF-ad libitum); D2: Red Lentil Straw (RLS-ad libitum)+ Lamb Mixed Feed (LMF-ad libitum); D3: 50% wheat straw +50% red lentil straw (WRWS-ad libitum) + Lamb Mixed Feed (LMF-ad libitum). The result of the study indicated that the live weight, live weight gain and serum minerals of lambs were not different among the feeding groups. In conclusion red lentil straw or red lentil + wheat straw mixtures as ad libitum are used much successfully than that of wheat straw for economic lamb production of Awassi breed.
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    The effects of Mannheimia haemolytica and albendazole on marbofloxacin pharmacokinetics in lambs
    (Springer, 2019) Altan, Feray; Ipek, Duygu Neval Sayin; Corum, Orhan; Alp, Simten Yesilmen; Ipek, Polat; Uney, Kamil
    The study aimed to define the effects of M. haemolytica and a single oral dose of albendazole on the single-dose pharmacokinetics of marbofloxacin in lambs. The pharmacokinetic-pharmacodynamic integration of marbofloxacin was applied to describe a 3 mg/kg intramuscular dose in lambs. The 6 healthy and 12 naturally infected with M. haemolytica lambs (Akkaraman, males weighing 10-15 kg and aged 2-3 months) were used in this study. In the marbofloxacin group, 6 healthy lambs received marbofloxacin. In the albendazole group after 2 weeks washout period, the same animals received marbofloxacin on 1 h after albendazole. In the diseased marbofloxacin group, 6 lambs naturally infected with M. haemolytica received marbofloxacin. In the diseased albendazole group, 6 lambs naturally infected with M. haemolytica received marbofloxacin on 1 h after albendazole. The marbofloxacin and albendazole were administered each as a single dose of 3 mg/kg intramuscular and 7.5 mg/kg oral, respectively, in the respective groups. Plasma concentration of marbofloxacin was measured with HPLC-UV and pharmacokinetic parameters were analyzed by non-compartmental model. Albendazole did not change the pharmacokinetic profiles of marbofloxacin in healthy and diseased lambs. However, M. haemolytica affected the pharmacokinetics of marbofloxacin in diseased lambs, AUC(0-24)/MIC90 ratio was not found to be higher than 125, but C-max/MIC90 ratios was found to be higher than 10 for an MIC value of 0.25 mu g/mL in all groups. The marbofloxacin dose described in this study may not be effective for the treatment of infections due to M. haemolytica in lambs, with MIC <= 0.25 mu g/mL.
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    Potentilla fulgens root extract’s effect on breast cancer (MCF-7) and osteosarcoma (U2OS) cells proliferation and migration
    (2024) Ipek, Polat; Baran, Ayse
    Aim: This study evaluates the cytotoxic effect of Potentilla fulgens root extract on MCF7, U2OS, and RPE-1 cell lines and investigates its impact on cell migration. Materials and Methods: The effect of Potentilla fulgens root extract on the cell viability of MCF-7, U2OS, and RPE-1 cells was analyzed using an MTT assay. The effect of the compounds on cell migration was evaluated at 24 and 48 hours using the wound healing assay. A wound-healing assay was used to measure the metastatic ability of cells in vitro. Results: MTT assay showed that PRE had a cytotoxic effect on all three cell lines, depending on dose and time, and this effect was statistically significant. The wound healing assay results showed that PRE slowed the migration of live MCF-7 and U2OS cancer cells, and these effects increased over time. Conclusion: As a result, this study indicates that PRE may be very useful in treating human osteosarcoma and breast cancer.