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Öğe Environmentally friendly silver nanoparticles synthesized from Verbascum nudatum var. extract and evaluation of its versatile biological properties and dye degradation activity(Springer, 2024) Hazman, Ömer; Khamidov, Gofur; Yılmaz, Mustafa Abdullah; Bozkurt, Mehmet Fatih; Kargıoğlu, Mustafa; Tukhtaev, Davlat; Erol, İbrahimIn the present study, green synthesis of silver nanoparticles (VNE-AgNPs) via Verbascum nudatum extract was carried out for the first time. The synthesized AgNPs were characterized by different spectral methods such as UV–vis, FTIR, XRD, TEM, and EDAX. According to TEM analyses, the average size range of AgNPs was 17–21 nm, and the dominant peaks in the 111°, 200°, 221°, and 311° planes in the XRD pattern indicated the Ag-NPs FCC crystal structure. FTIR data showed that VNE-AgNPs interacted with many reducing, capping, and stabilizing phytochemicals during green synthesis. VNE-AgNPs had higher antibacterial activity against S. aureus and E. coli bacterial strains with a maximum inhibition zone of 21 and 18 mm, respectively, than penicillin 5 IU, used as a positive control in the study. The cytotoxic effect of VNE-AgNPs appeared at a concentration of 50 µg/mL in L929 cells and 5 µg/mL in cancer (A549) cells. When the impact of VNE-AgNPs and C-AgNPs on inflammation was compared, it was found that VNE-AgNPs increased TNF-α levels (333.45 ± 67.20 ng/mg-protein) statistically (p < 0.05) more than TNF-α levels (256.92 ± 27.88 ng/mg-protein) in cells treated with C-AgNPs. VNE-Ag-NPs were found to have a degradation efficiency of 65% against methylene blue (MB) dye within 3 h. Graphical Abstract: (Figure presented.)Öğe Green synthesis of Ag nanoparticles from Verbascum insulare Boiss. and Heldr.: Evaluation of antimicrobial, anticancer, antioxidant properties and photocatalytic degradation of MB(Elsevier B.V., 2024) Hazman, Ömer; Khamidov, Gofur; Yılmaz, Mustafa Abdullah; Bozkurt, Mehmet Fatih; Kargioğlu, Mustafa; Savrik, Mehmet; Tukhtaev, Davlat; 0000-0002-2702-6847; 0000-0001-7347-1522; 0000-0002-4090-7227; 0000-0002-2427-2225; 0000-0001-7239-2427In recent years, due to the environmental friendliness of nanoparticle production, biosynthesis methods using plant extracts and possible physical, chemical, thermal and biological properties of the synthesized nanoparticles are frequently the subject of research. In the present study, hydrothermally assisted synthesis of Ag nanoparticles using twig-leaf extract of Verbascum insulare Boiss. and Heldr., (VIE-AgNPs) a plant endemic to Turkey, was successfully carried out for the first time in this study. The presence of 24 of the 53 phytochemicals investigated in the content of the extract of Verbascum insulare Boiss. and Heldr. (VIE) determined by the LC-MS/MS system was detected. In addition, the amount of phenolic substance involved in reduction was found to be 60.42 ± 9.11 µg Gallic acid equivalent/mg-extract. The synthesized AgNPs were characterized by UV–visible spectra (UV–vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transforms infrared spectroscopy (FTIR), and X-ray diffraction (XRD). In particular, the surface plasmon band observed at 422 nm, the characteristic peaks observed in XRD, and the bond energy values seen in XPS strongly confirmed the formation of AgNPs. XRD and TEM data showed that AgNPs had an average size range of 25–30 nm. FTIR data indicated the presence of some herbal agents on the surface of AgNPs. The properties of the VIE-AgNPs were compared in vitro with chemically produced AgNPs (C-AgNPs). The OSI level of C-AgNPs was 87.78 ± 1.75 AU, while that of VIE- AgNPs was 110.42 ± 7.54 AU. It was observed that the nanoparticle and VIE used in the study had antimicrobial activity. It was determined that its antibacterial activity was stronger than its antifungal activity. When the analyzed inflammatory parameter levels (TNF-?, IL1?, TGF-?, and DEF-?2) were examined, the effect of VIE on inflammation in A549 cells could not be detected. However, it was determined that VIE-AgNPs, one of the nanoparticles used in the study, increased anti-inflammatory levels more than C-AgNPs. When the received data are evaluated together, it can be said that biosynthesized AgNPs suppress proliferation and stimulate apoptosis in cancer cells by increasing oxidative stress and inflammation levels compared to chemical AgNPs, thus showing an anticarcinogenic effect. It was found that VIE-AgNPs could effectively degrade the water pollutant methylene blue (MB) dye. VIE-AgNP mediated 78 % photocatalytic degradation of MB in 180 min. All superior biological properties are thought to be due to the low particle size and the interaction of active components with AgNPs during the biosynthesis process. Because it has been observed that VIE-AgNPs, which have a smaller particle size, have higher biological (cytotoxic, anticarcinogenic, inflammatory, etc.) activities than C-AgNPs. It can be said that AgNPs produced by biosynthesis may have potential use in the production of environmentally friendly, antibacterial/anticarcinogenic materials.Öğe Phytochemical profile, antioxidant and antimicrobial potency of aerial parts of Salvia tomentosa Miller(Slovensko Kemijsko Drustvo, 2023) Balkır, Şehnaz; Hazman, Ömer; Aksoy, Laçine; Yılmaz, Mustafa Abdullah; Çakır, Oğuz; Kara, Recep; Erol, İbrahimAntioxidant activity, antimicrobial potency and components of the aerial parts (leaf, stem, flower and mixture) of Salvia tomentosa Miller were determined qualitatively and quantitatively in this study. Aqueous extracts of Salvia tomentosa(ST) were prepared by using the flower, leaf and stem parts and all the above-ground parts of the plant (flower-leaf-stem mixture) for this purpose. The radical scavenging activity, total antioxidant/oxidant status, antimicrobial potential, phenolic substances and qualitative/quantitative analyzes of the components in the extracts were determined. ST-stem phenolic acid amount (599 ± 34 mg gallic acid equivalent (GAE)/g extract) was found to be close to the standard sub-stance caffeic acid (651 ±3 1 mg GAE/g extract). Total antioxidant status of ST-mix (3.4 ± 0.1 mmol Trolox Equiv./L) and ST-stem (3.4 ± 0.1 mmol Trolox Equiv./L) and natural antioxidant Vitamin C (3.6 ± 0.1 mmol Trolox Equiv./L) were not statistically different. The extract produced by using S. tomentosa aerial parts (flower-stem-leaf ) showed stronger antioxidant and antimicrobial activity than the aqueous extracts obtained separately from the flower, stem and leaf of the plant. However, it was determined that the components of the separately prepared flower, stem and leaf extracts and the extract components obtained from the aerial parts were largely similar. At the same time, it was observed that there were significant differences in the presence of these components.
