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    Alpha-Amylase Production by Bacillus subtilis RSKK96 in Submerged Cultivation
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011) Akcan, Nurullah; Uyar, Fikret; Guven, Aysel
    The bacterial strain Bacillus subtilis RSKK96 was shown to produce extracellular alpha-amylase. Enzyme synthesis occurred at 72 h with an optimum of 37 degrees C. Effects of various carbon, nitrogen, amino acid sources and chemicals on alpha-amylase production were examined. FeSO4, ZnSO4 and CuSO4 inhibited bacterial growth as a result, amylase production. Maximum alpha-amylase production (858.6 +/- 41.9 U/mg) was obtained in a medium containing 0.5% cotton stalk in 72 h.