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Öğe A comparative investigation of the effects of Resveratrol and dental pulp delivered mesenchimal stem cells on rat tibia bone defect healing(Univ Zulia, Facultad Ciencias Veterinarias, 2024) Agin, Hatice Demircan; Gunes, Nedim; Guler, RidvanResveratrol (3,4,5-trihydroxystilbene), an antioxidant compound, has a natural phytoalexin structure and also has many properties such as anti-inflammatory, antineoplastic and antiplatelet. In addition, mesenchymal stem cells isolated from various tissues are considered as a potential cell source for bone regenerative therapies. The present study aims to examine the effects of Resveratrol and dental pulp- derived mesenchymal stem cells on new bone formation in rats, both isolated and combined, by immunohistochemical methods. Twenty eigth Spraque Dawley male rats were used in the study. The rats were divided into four groups with seven rats in each group; the control group (Group 1) (n=7), the Systemic Resveratrol group (Group 2) (n=7), the Stem cell group (Group 3) (n=7), the Stem cell + Systemic Resveratrol group (Group 4) (n=7). A defect was opened on the tibia bones of the rats in all groups with a trephane bur (diameter of 3 mm and a length of 4 mm). After the 4-week experiment, all rats were sacrificed following the experimental protocols specific to each group. The specimens of tibia were subjected to histomorphological examination in fixative solutions. Values of inflammation, connective tissue formation, osteoclastic activity, osteoblast values, new bone formation, BMP2 and BMP4 expression levels obtained for all groups were evaluated by statistical analysis. Compared to the control group, new bone formation and osteoblastic activity were found to be significantly higher in the Stem cell group and Stem cell + Systemic Resveratrol group. ( P =0.001) Additionally, new bone formation in the Systemic Resveratrol group was found to be significantly lower than in the Stem cell + Systemic Resveratrol group. ( P =0.006) No significant difference was observed between other groups. ( P >0.05) According to the results of the study, it was observed that Stem cell + Resveratrol treatment was more effective than isolated Resveratrol or isolated stem cell treatment applications, it induced the development of more bone trabeculae, decrease inflammation and increased the number of osteoblasts involved in bone formation. In the light of these data, it was concluded that the combined use of Resveratrol and Stem cells is more effective on the healing of bone defects than their isolated use.Öğe Evaluation of Attitudes and Perceptions in Students about the Use of Artificial Intelligence in Craniomaxillofacial Surgery(Lippincott Williams and Wilkins, 2024) Guler, Ridvan; Yalcin, Emine; Gulsun, BelginDevelopments in technology have created great changes in the field of medicine and dentistry. Artificial intelligence technology is one of the most important innovations that caused this change. This study aimed to evaluate the opinions of dentistry students regarding the use of artificial intelligence in dentistry and craniomaxillofacial surgery. Two hundred ninety-six dentistry students between the ages of 19 and 30 participated in the study. Participants submitted the survey by e-mail examining the student's opinions and attitudes regarding the use of artificial intelligence in dentistry and craniomaxillofacial surgery. Respondents' anonymity was ensured. 47.30% (n: 140) of the students participating in the study are fourth-year students, and 52.70% (n: 156) are fifth-year students. While 48.98% (n: 145) of the participants have knowledge about the uses of artificial intelligence in daily life, 28.37% (n: 84) of the students have knowledge about robotic surgery. While 74% of the participants think that artificial intelligence will improve the field of dentistry and craniomaxillofacial surgery, it has been observed that they are not worried about these applications replacing dentists in the future. It was determined that there was no statistically significant difference between fourth-year and fifth-year students in their knowledge levels about the areas of use of artificial intelligence (P=0.548). Students' opinions show that 74% agree that artificial intelligence will lead to major advances in the field of dentistry and craniomaxillofacial surgery. This shows the relationship between dentists and artificial intelligence points to a bright future. © 2024 Mutaz B. Habal, MD. Unauthorized reproduction of this article is prohibited.Öğe Investigation of the Effects of Thymoquinone and Dental Pulp-Derived Mesenchymal Stem Cells on Tibial Bone Defect Models(Lippincott Williams & Wilkins, 2024) Ozden, Ersin; Kaya, Beyza; Guler, RidvanThe thymoquinone obtained from Nigella sativa increases osteoblastic activity and significantly reduces the number of osteoclasts, thereby accelerating bone healing. In addition, mesenchymal stem cells isolated from various tissues are considered a potential cell source for bone regenerative therapies. The aim of this study is to investigate the effectiveness of thymoquinone, a current and novel agent, in combination with mesenchymal stem cells derived from the dental pulp in promoting bone healing. In the study, 28 male Sprague Dawley rats were used. The rats were divided into 4 groups, each consisting of 7 rats: the control group (group 1) (n=7), thymoquinone group (group 2) (n=7), stem cell group (group 3) (n=7), stem cell+thymoquinone group (group 4) (n=7). A bone defect of 4 mm in diameter and 5 mm in length was created in the left tibial bones of all rats with a trephine bur. In group 1, no procedure was applied to the defect area. Group 2 was applied thymoquinone (10 mg/kg) with oral gavage. In group 3, stem cells were used locally to the defect area. In group 4, stem cells and thymoquinone (10 mg/kg) was applied to the defect area. All rats were killed on the 28th day of the experiment. Tibia tissues extracted during sacrifice were histomorphologically examined in a fixative solution. Significant differences were found in terms of new bone formation and osteoblastic activity values in the thymoquinone (P<0.05), stem cell (P<0.05), and stem cell+thymoquinone (P<0.05) groups compared to the control group. In addition, while there was no significant difference in the thymoquinone group compared to these stem cell+thymoquinone group in terms of osteoblastic activity (P>0.05), the difference in terms of new bone formation was found to be significantly lower. No significant differences among the other groups were observed in new bone formation and osteoblastic activity (P>0.05). According to the results of our study, stem cell+thymoquinone treatment for bone defects is not only more effective than thymoquinone or stem cell treatment alone but also induces greater development of bone trabeculae, contributes to the matrix and connective tissue formation, and increases the number of osteoblasts and osteocytes involved in bone formation.
