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    Association between saliva quantity and content parameters with caries intensity levels: A cross-sectional study among subcarpathian children
    (Association of Support to Oral Health Research (APESB), 2019) Bilyschuk, Liubov M.; Andrii, Keniuk; Goncharuk-Khomyn, Myroslav; Yavuz, İzzet
    Objective: To evaluate saliva quantity and content parameters among children of 7 and 12 years old, who permanently living on the territory of Subcarpathia with the registered territoryassociated fluoride deficiency in the water, and their association with the caries status of pediatric patients. Material and Methods: The study sample was formed of 48 children (22 of 7 years old and 26 of 12 years old). The content of calcium in the oral liquid was determined by the ocresolphthalein complexone method. Estimation of concentration rate and fluoride activity in the oral liquid was carried out by using the ion-selective electrode ELIS-131 F and ionometer EV-74. The content of inorganic phosphorus in saliva was determined using the phosphorus reaction with molybdic acid. Results: Among all study samples, 18.8% were registered with low caries intensity level (DMF = 1.55 ± 0.16), 33.3% with moderate caries intensity level (DMF = 3.94 ± 0.29), and 47.9% with high caries intensity level (DMF = 9.05 ± 1.11). During the comparison of calcium content and mineralization coefficient values between children with low and high caries intensity levels registered difference was statistically significant (p<0.05), while for salivary flow rate parameter such difference was no significant (p>0.05). Between children with normal salivary flow rate, and children with a lowered salivary flow rate there was no statistical difference in such parameters as fluoride concentration, calcium content, phosphorus content and calcium-phosphorus balance (p>0.05). Conclusion: Caries intensity levels were more statistically associated with parameters of calcium content in saliva and related mineralization coefficient, rather than with the average salivary flow rate.
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    Can the Density of Mineralized Dental Tissues (Dentin and Enamel) Be Measured and Compared with 3D Cone Beam Computed Tomography in Cases of Ectodermal Dysplasia?
    (Int Scientific Information, Inc, 2022) Yavuz, Yasemin; Akleyin, Ebru; Dogan, Mehmet Sinan; Goncharuk-Khomyn, Myroslav; Akkus, Zeki
    Background: Since 3-dimensional cone beam computed tomography (CBCT) started to be used in dentistry, mineral density can now be examined with computer software from the data on the images obtained. Detailed and clear im-ages at different slice intervals can be obtained with CBCT, and mineral density can be measured from the im-age data on a computer with a Hounsfield unit (HU) scale. In addition to the broad opportunities presented by CBCT, this feature has presented a wider perspective to researchers. Material/Methods: In this study, the CBCT images obtained from patients with the genetic disorder of ectodermal dysplasia were compared with the images of a control group to determine differences in mineralization of the teeth and to show that these could be compared by measuring the mineral density of dentin and enamel tissues using the HU scale on data from CBCT images. This opens new opportunities for cognitive and implementation research. Results: In the study, CBCT images of 14 ectodermal dysplasia and 14 control group cases previously obtained for vari-ous reasons were used. Mineral density measurements were made from 4 different regions of the teeth of the ectodermal dysplasia and control groups (incisor edge of the crown, the center buccal, cervicale line, and apex of the teeth), and the groups were compared. Conclusions: The aim of this study was to provide a new overview of the feasibility and suitability of mineralization mea-surement of dentin and enamel dental tissues with CBCT in ectodermal dysplasia and control groups.
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    Comparison of bacterial load parameters in subgingival plaque during peri-implantitis and periodontitis using the RT-PCR method
    (University of Zagreb, 2020) Nastych, Oksana; Goncharuk-Khomyn, Myroslav; Foros, Anatoliy; Cavalcanti, Alessandro; Yavuz, İzzet; Tsaryk, Vladyslav
    Objective: To estimate the actual parameters of bacterial load in subgingival plaque during periodontitis and peri-implantitis pathologies using the RT-PCR (real-time polymerase chain reaction) method and evaluate their associations with clinical periodontal indicators. Materials and Methods: Five different groups of subjects were selected according to a formulated design of the study: with mild/ moderate periodontitis, with severe periodontitis, with peri-implantitis, healthy periodontal group and healthy peri-implant group. Subgingival plaque samples were formed with paper points inserted in the pocket/sulcus area for 30 seconds. A standardized test the “ParodontoScreen” was provided for identification of target opportunistic pathogens (A. actinomycetemcomitans, P. gingivalis, T. forsythia, P. intermedia, T. denticola) by the RT-PCR. Results: Bacterial load parameters demonstrated a significant tendency towards an increase within periodontitis progression and during the presence of peri-implantitis pathology. Each targeted mean bacterial load level was statistically associated with periodontitis or peri-implantitis pathology (p < 0, 05) according to the provided univariate analyses and upon condition that bacterial load parameters of healthy sites were used as reference for equiparation. The highest correlation values were found between periodontal probing depth and bacterial load parameters of A. actinomycetemcomitans (r=0, 37; p < 0, 05) and P. gingivalis (r=0, 28; p < 0, 05); and also between clinical attachment loss and bacterial load values of A. actinomycetemcomitans (r=0, 38; p < 0, 05) and P. gingivalis (r=0, 24; p < 0, 05). Conclusions: Periodontitis and peri-implantitis are associated with the same microbial pathogens even though the distribution pattern of their bacterial load and detection frequency parameters registered with RT-PCR could be distinct and linked to the individual patient-related conditions and the severity stage of pathology.
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    The effect of final irrigation agents on push-out bond strength of calcium silicate-based cements to dentin
    (2023) Yeniçeri Özata, Merve; Falakaoğlu, Seda; Batur, Muharrem; Adıgüzel, Özkan; Goncharuk-Khomyn, Myroslav
    Aim: This aim of this study was to compare the effects of different chelating agents [ethylenediaminetetraacetic acid (EDTA), glycolic acid (GA) and citric acid (CA)] on the push-out bond strength (POBS) of two calcium silicate-based silicate cements (CSC) (Biodentine and PD MTA White). Methodology: Dentin discs of 1 ± 0.2 mm thickness were taken from the middle root region of thirty-nine extracted mandibular premolar teeth and two holes (1 mm diameter) were drilled in each disc (n = 78 holes). The samples were then randomly divided into three groups (n = 26 holes) according to the final irrigation agents: Group 1: 20% CA, Group 2: 17% EDTA, Group 3: 10% GA. Then, two different materials were applied to the holes in each group (n = 13 holes): a: PD MTA White, b: Biodentine. POBS test was performed at a crosshead speed of 0.5 mm/min. The dentin discs were examined under stereomicroscope (25×) to assess the bond failure type. Data were analyzed using a two-way analysis of variance (ANOVA) and comparison of main effects was examined with the Bonferroni test, and multiple comparisons were analysed with the Tukey HSD test. The level of signifcance was 5%. Results: Biodentine showed significantly higher POBS than PD MTA White (p < 0.05). There was no significant difference between the final irrigation agents (p > 0.05). CA – Biodentine group showed substantially higher POBS than EDTA – PD MTA White, CA – PD MTA White, and GA – Biodentine groups (p < 0.05). Conclusion: While CA increased the POBS of Biodentine significantly, the POBS of PD MTA White was not affected by the final irrigation agents.
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    Key aspects of dental diagnostics and treatment specifics in ectodermal dysplasia patients: Comprehensive literature review
    (Termedia Publishing House Ltd., 2020) Yavuz, İzzet; Goncharuk-Khomyn, Myroslav; Cavalcanti, Alessandro Leite; Boykiv, Alina B.; Nahirny, Yaroslav
    Introduction: Pathogenesis of ectodermal dysplasia (ED) is explained by alterations occurring within derivates of ectoderm, caused by different genetic violations. Objectives: The aim of this study was to provide critical comprehensive literature review considering diagnostic and treatment specifics among dental patients with ED. Material and methods: Comprehensive literature search was provided via PubMed database (https://pubmed. ncbi.nlm.nih.gov/) and Google Scholar search engine (https://scholar.google.com/). Systematized number of publications was evaluated as a subject of manual content analysis, categories of which included dental diagnostic and treatment aspects of patients with ED. . Results: Treatment approaches for remaining deciduous and permanent teeth under the condition of future prosthodontic rehabilitation planning differ in numbers of publications dedicated to the topic of dental care provision among ED patients. Such non-consistency could be explained by diversities in described prosthetic rehabilitation protocols, and initial variations of teeth’s form, structure, developmental stages, and their overall quantity registered during primary examination of ED dental patients, including possible alterations in enamel integrity, dispersions in pulp chamber volume, caries intensity levels, changes in root formation stages, and eruption phases during ED. Conclusions: The most prominent dental and oral signs of ED that potentially could be used as criteria for diagnostics with varying degree of diagnostic significance, include oligodontia, delayed eruption and development of teeth, changed tooth crown and/or root morphology, decreased salivary function, cleft lip and/or palate. However, there is no unified algorithm of ED dental patients’ rehabilitation, and planning of such should consider a number of initial clinical situation’s factors, ED manifestations, individual functional prognosis, and esthetic demands.