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Öğe Effect of iron limitation on the amount of slime produced by strains of Staphylococcus epidermidis.(1995) Elçi S.; Atmaca S.; Gül K.To investigate the effect of iron limitation on the amount of slime production by most strains of Staphylococcus epidermidis, ethylenediamine-di-o-hydroxyphenol acetic acid (EDDA), was added to the growth medium and twenty clinically relevant strains were assayed for slime production during growth in standard tryptic soy broth (TSB) and iron-limited TSB. Findings revealed that the density of the biofilm which formed on the base of microtitre plates was dependent on the degree of iron limitation, characteristics of the test strains and the stage of the growth cycle.Öğe Evaluation of malaria cases in Diyarbakir between 1999 and 2004(2006) Temiz H.; Gül K.In this study; we investigated the prevalence and distribution of malaria in the city of Diyarbakir, retrospectively. The investigation was carried out according to age, gender and areas of settlement between 1999 and 2004. The records were obtained from the Health Directorship of the Ministry of Health in Diyarbakir. A total of 22,062 malaria cases (11,643 male, 10,419 female) had been reported during a period of five years. Compared to previous years, the prevalence of malaria had significantly decreased. However; since the geographic conditions in Diyarbakir are suitable for the reproduction of Anopheles spp. and malaria is still endemic in Diyarbakir, the fight against malaria should be continued.Öğe Evaluation of the Ehrlich-Ziehl-Neelsen (EZN) and amplified Mycobacterium tuberculosis direct test according to the BACTEC method in respiratory and nonrespiratory samples(2007) Özekinci T.; Meşe S.; Atmaca S.; Akpolat N.; Gül K.Aim: Tuberculosis remains a significant and threatening disease, particularly in developing countries. Mycobacterium tuberculosis should be detected and identified as soon as possible to ensure the prevention of the spread of the disease. For this purpose, use of fast and reliable laboratory diagnostic methods with high sensitivity and specificity was initiated in recent years. Materials and Methods: In this study, 107 respiratory and 198 nonrespiratory (305 in total) samples submitted to Dicle University Faculty of Medicine Clinical Microbiology Laboratory were examined using the Ehrlich-Ziehl-Neelsen (EZN), BACTEC 460 TB (Becton and Dickinson Diagnostic Instrument System, Towson, MD), and MTD (Amplified Mycobacterium tuberculosis Direct Test, Gen-Probe, USA) methods. Results: In respiratory samples, sensitivity of EZN was found as 83.33%, specificity as 95.04%, positive predictive value as 50%, and negative predictive value as 98.96%, whereas in nonrespiratory samples these values were 18.18%, 98.39%, 40%, and 95.37%, respectively. In respiratory samples, sensitivity of MTD was found as 83.33%, specificity as 94.05%, positive predictive value as 45.45%, and negative predictive value as 98.95%, whereas in nonrespiratory samples these values were 54.54%, 88.23%, 21.42%, and 97.05%, respectively. Conclusions: In view of the above, the pre-diagnostic EZN test and the MTD method based on nucleic acid amplification should be applied together with the BACTEC 460 system, which is considered as a gold standard, and the evaluation should be made accordingly. Furthermore, MTD should not be used as a screening test due to its high cost, and should rather be preferred in smear-positive samples. © TÜBİTAK.Öğe Isolation, identification and antibiotic susceptibility of Campylobacter spp. in aborted sheep fetuses(2007) Yeşilmen S.; Gül K.The aim of this study was to isolate identify and detemine the antibiotic susceptibility of Campylobacter spp in aborted sheep fetues in Diyarbakir and its vicinity. The bacteria were isolated by conventional methods. Biochemical, growth and tolerance characteristics of the organism were used to identify the isolates. A total of 100 aborted fetues from 25 different locations were investigated and 10 (10%) yielded Campylobacter spp. Of the isolated bacteria, 7(7%) were C. fetus subsh. fetus and 3 (3%) were C. jejuni. All isolates were resistant to penicillin and susceptible to enrofloxacin; 9 isolates were inermediately susceptible to streptomycin, 8 isolates were intermediately susceptible to tetracycline and susceptible to gentamycin and neomcyin and 7 isolates were susceptible to erythromycin.Öğe Tetanus seroprevalence among pregnant women in ben-u sen health center in diyarbakir(Gulhane Military Medical Academy, 2011) Ceylan A.; Çöplü N.; Saka G.; Gül K.; Sönmez C.; Esen B.; Bozyel O.A.AIM: At the aim of this study was to determine the tetanus seroprevalence among pregnant women and childbearing aged woman living in the Ben-u Sen Health Center region that is in lower socio-economical level. METHOD: In this descriptive study, a team including the staff of health center and several volunteers visited the houses of pregnant women living in the health center coverage region and questionnaires were completed through face to face interviews. The study group included 214 pregnant women. Among them, serum samples of 197 subjects' were studied for anti-toxic antibody for tetanus. For control, serum samples from 200 women living in the same health center region were collected. It was evaluated as partially protective, protective and longterm protection when tetanus antibody level was 0,01-<0,1 IU/ml, 0,1-<1.0 IU/ml and 1,0 IU/ml and over, respectively. RESULTS: The mean age of the women was 26.4, mean marriage and first pregnancy ages were 17.9 and 18.9, respectively, and 40% of the subjects had never been examined or received follow up by a health center. It was revealed that 25.8% of the subjects were not protected and 74.2% had a full protection level of antibody. Within the control group, the same levels of antibodies were detected in 40.0% and 60.0% of the women, respectively. CONCLUSION: The study indicates that the immunity levels against tetanus are not satisfactory and every childbearing aged woman should be included in a vaccination program whenever they receive any examination in a health center.Öğe Three cases of chronic suppurative otitis media (CSOM) caused by kerstersia gyiorum and a review of the literature(EDIMES Edizioni Medico Scientifiche, 2018) Özcan N.; Saat N.; Baylan M.Y.; Akpolat N.; Atmaca S.; Gül K.New identification techniques such as gene sequencing and mass spectrometry have increased the incidence of novel agents such as Kerstersia gyiorum. As a new member of the Alcaligenaceae family, K. gyiorum was isolated from wounds, respiratory tract, urine specimens and most frequently from chronic suppurative otitis media (CSOM). We isolated three K. gyiorum strains from three CSOM cases over a one-year period. The strains were analyzed by mass spectrometry and identified by Bruker Biotyper 3.1 (Bruker Daltonics, USA). The cases were young patients without chronic diseases and immuno-deficiencies. Two strains were resistant to ciprofloxacin. © 2018, EDIMES Edizioni Medico Scientifiche. All rights reserved.Öğe Typhoid fever outbreak in Ahmetli village, Ergani-Diyarbakir(2003) Ceylan A.; Acemo?lu H.; Hoşo?lu S.; Gül K.; Ilçin E.; Efe M.Salmonella typhi leads to typhoid fever outbreaks due to the contamination of drinking water. In this study, a typhoid fever outbreak due to drinking water contamination in Ahmetli willage, Ergani-Diyarbakir, in the period of December 25, 2001 - January 4, 2002 was evaluated. A total of 181 suspicious cases were admitted to different health care centers during the outbreak and 71 (39.2%) of them were hospitalized. Gruber-Widal test could be performed for only 8 hospitalized patients, and were found positive in 6 of them. S.typhi was isolated from 3 of the 26 blood cultures and two of the 73 stool cultures of the patients. All village inhabitants were visited and suspicious cases were screened for salmonellosis. It was shown that the village-drinking water was contaminated with sewage. No S.typhi was isolated from the drinking water since it was previously disinfected. Maintenance of drinking water supply system, individual chloride usage and education were recommended, as safety causions.
