Yazar "Fincan, S. Aguloglu" seçeneğine göre listele

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    Investigation of some radiation interaction parameters for bacteria isolated from the soil in the low energy region
    (Canadian Science Publishing, 2020) Akman, F.; Enez, B.; Fincan, S. Aguloglu; Akdemir, F.; Gecibesler, I. H.
    The purpose of this work, investigation of radiation attenuation characteristics, such as mass attenuation coefficient (mu/rho), molar extinction coefficient (epsilon), effective atomic number (Z(eff)), and electron density (N-E), of isolated bacteria from soil samples taken from Diyarbakir in Turkey. The experiments were performed using a narrow beam geometry with the help of the a high-resolution Si(Li) detector and three different radioactive sources at 15 different photon energies. The epsilon, Z(eff), and N-E values for some bacteria isolated from soil, such as Bacillus megaterium, Bacillus simplex, Bacillus subtilis, and commercially purchased Geobacillus stearothermophilus bacterium, were obtained from the measured mu/rho results in the energy range from 13.94 to 88.04 keV. The experimental values were checked with two different theoretical results and good agreement was observed among them. The results show that Bacillus simplex bacterium has the highest values of mu/rho and Z(eff) and Bacillus subtilis bacterium has the biggest values of epsilon and N-E among the present bacteria.
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    Öğe
    Isolation and partial characterization of alpha-amylase from Klebsiella pneumoniae and Enterobacter sp.
    (Wiley-Blackwell Publishing, Inc, 2009) Matpan, F.; Fincan, S. Aguloglu
    [Abstract Not Available]
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    Öğe
    Purification and characterization of thermostable ?-amylase from thermophilic Anoxybacillus flavithermus
    (Elsevier Sci Ltd, 2014) Fincan, S. Aguloglu; Enez, B.; Ozdemir, S.; Bekler, F. Matpan
    This study reports on the purification and characterization of thermostable alpha-amylase (alpha-1-4 D-glucan glucanohydrolase EC 3.2.1.1) from a newly isolated Anoxybacillus flavithennus. A. flavithermus was used, which was isolated from hot water springs of Omer, Afyonkarahisar, Turkey. The gram-positive, spore-forming, motile, moderately thermophilic bacteria were found to be a strain of A. flavithermus analysed by 16S rRNA comparison. The optimal conditions for bacterial growth were determined to be at 20th h, 55 degrees C and pH 6.0. Maximum alpha-amylase activity was obtained at 55 degrees C at pH 7.0 after 24h of incubation. Thermostable alpha-amylase from A. flavithermus was purified by 70% (NH4)(2)SO4 and ion-exchange chromatography (5.2-fold; 65.8% yield). The molecular weight of alpha-amylase was 60 kDa, as estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The alpha-amylase hydrolyzed soluble starch at 55 degrees C with K-m: 0.005 mM and V-max: 3.5 mu mol min(-1). (C) 2013 Elsevier Ltd. All rights reserved.