Yazar "Ersali, Yusuf" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    Mass shoot proliferation of pistacia khinjuk stocks using temporary immersion bioreactor system (TIS)
    (International Society for Horticultural Science, 2014) Tilkat, Engin; Süzerer, Veysel; Ersali, Yusuf; Hoşer, Ayşe; Kılınç, Fatih Mehmet; Tilkat, Emine Ayaz; Akdemir, Hülya; Onay, Ahmet
    A temporary immersion bioreactor system (TIS), compared to semi-solid systems has many advantages such as (1) the system can be controlled by an automation system; (2) a greater number of explants could be used at the same time and (3) there is no need to use an organic gelling agent. In this study, we aimed to develop a method suitable for mass shoot proliferation of P. khinjuk Stocks. Shoot tips and nodal segments of in vitro grown axenic regenerates derived from seedlings of Pistacia khinjuk Stocks were used as material. In both semi-solid and liquid medium experiments, an MS medium supplemented with 1 mg/L BA and 30 g/L sucrose was used. Various immersion frequencies (2, 4, 8, 16 or 20 h) and immersion times (5, 10 or 15 min) were tested on the TIS system. Repeated trials for immersion frequencies at short time intervals (10 min/2 h, 10 min/4 h etc.) showed hyper hydrated explants up to 100%, while this problem was not observed in immersions with relatively longer time intervals (10 min/20 h). The highest shoot forming capacity index (3.12) and the minimum hyper hydration rate (8%) were obtained in shoot tips with 10 min immersion frequency every 16 h. The protocol presented here, presents the initial stage of mass propagation of P. khinjuk Stocks cultures.
  • [ X ]
    Öğe
    Micropropagation of the pistachio and its rootstocks by temporary immersion system
    (Springer, 2014) Akdemir, Hulya; Suzerer, Veysel; Onay, Ahmet; Tilkat, Engin; Ersali, Yusuf; Ciftci, Yelda Ozden
    Although several studies have been reported on the micropropagation of the pistachio and its rootstocks, to date none of them had been efficient on the mass production of these plants in bioreactor systems. Thus, the micropropagation of juvenile pistachio shoot tips and nodal buds was investigated in a temporary immersion bioreactor system (RITA(A (R))) and on a conventional semi-solid medium. Among the tested immersion conditions, immersion for 24 min every 16 h reduced vitrification and improved proliferation in the pistachio. Interactions were evident in immersion time and frequency in nodal segments. Nodal buds were better than shoot tips as the highest multiple shoot formation was recorded in MS medium containing 4 mg L-1 BA and 0.1 mg L-1 GA(3) in RITA(A (R)). Although shoot tip necrosis (STN) was observed in shoots proliferated on semi-solid MS medium, such a symptom did not occur in shoots sprouted in the RITA(A (R)). Additionally, these optimized conditions were applied to nodal buds of mature male pistachio 'AtlA +/- aEuro (TM) and Pistacia rootstocks (P. khinjuk Stocks and P. atlantica Desf.), and the micropropagation in the bioreactor system, in comparison to the semi-solid medium, was also improved. Furthermore, in vitro rooting of pistachio plantlets, despite the lower range (27.5 %), was also achieved in RITA(A (R)). However, rooting was better on semi-solid medium for all tested species (ranged between 50 and 70 %). The results of this study showed that RITA(A (R)) could be used for the mass propagation of pistachio and its rootstocks, as well as for other woody plant species.