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    HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS
    (Rojan GÜMÜŞ, 2019) Erdogan, Gamze; Nergiz, Yusuf; Ağaçayak, Elif
    The placenta plays a role in the pathophysiology of preeclampsia. Preeclampsia is more common in multifetal pregnancies than singleton pregnancies. In this study, we aimed to investigate the histopathology of normotensive and preeclamptic placentas and the localization of alkaline phosphatase activity. In our study, 10 normotensive and 10 preeclamptic, totally 20 placentas were obtained.Paraffin sections were stained with Hematoxylin-Eosin, Masson trichrome and PAS for histopathological examination.Remaining sections were then stained via Gomori’s method and micrographed under light microscope. Sections of the control group observed normal histologically structure.The  alkaline phosphatase reaction was evident in the inner and outer membranes of the syncytiotrophoblasts. There was a significant increase in the number of syncytial knots, terminal villi and syncytial bridges in the preeclamptic placenta sections. Marked thickening of the trophoblast basal membranes were observed. Alkaline phosphatase reaction in preeclampsia group: The localization of alkaline phosphatase in the inner and outer membranes of the syncytiotrophoblasts was reduced. In placentas of preeclampsia group, a significant increase in syncytial knot, syncytial bridge were observed. The level of alkaline phosphatase enzyme in preeclamptic placentas was found to be lower compared to normotensive placenta.
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    IMMUNOHISTOCHEMICAL ANALYSIS OF SFLT-1 AND ADAMTS-8 EXPRESSION IN DIABETIC RAT TESTIS TISSUE
    (Rojan GÜMÜŞ, 2019) Kaya, Seval; Nergiz, Yusuf; Asır, Fırat; Özkorkmaz, Ebru Gökalp; Erdogan, Gamze; Deveci, Engin
    Abstract: The aim of this study was to determine the testicular inflammation and angiogenetic effect of diabetes with ADAMTS-8 and SFlt-1 proteins immunohistochemically. Wistar albino male rats (n: 12)were used for the study, Group 1: Control group (n: 6), only 1 ml i.p. saline injection was performed. Group 2: Diabetes group (n: 6) received a single intraperitoneal dose of streptozotocin (STZ) of 60 mg / kg. The glucose value measured above 250 mg / dl was considered as diabetic. Under anesthesia, dissection was performed at the lower part of abdominal clearance and testicular tissue was removed. Testis tissues were fixed in 10% neutral formalin, followed by the routine paraffin protocol and cut with a microtome. Then, primary antibodies (ADAMTS-8, sFlt-1) were applied by immunohistochemistry method and incubated at +4 °C overnight. The sections were then examined under a light microscope. Diabetes group showed that Leydig cells in the  inter tubular area had vacuolization and capillary dilatations in histopathological examinations. sFlt-1 staining of control group showed positive expression in capillary endothelium between Leydig chambers of the intertubular area. Sflt-1 expression of diabetes group was observed in degenerative spermatic cells and Sertoli cells of the basement membrane facing tubules. The expression of ADAMTS-8in control group was positive in some Leydig cells in the interstitial area of Sertoli cells in seminiferous tubule but, spermatogenetic cells were negative. In the ADAMTS-8 staining of diabetes group, the expression of ADAMTS-8 was increased in the stromal cells and some inflammatory cells in the intertubular space. SFlt-1 plays a crucial role in angiogenesis as well as in diabetic testes and is marked as a precursor for the disruption of vascular structure and blood flow due to degenerative changes. It is thought that the distribution of ADAMTS-8 may be a determinant protein in the development of extracellular matrix and in damage to testicular tissue of diabetic testis.