Yazar "Erdal, Mehmet Emin" seçeneğine göre listele

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    Association of microRNA-related gene polymorphisms and idiopathic azoospermia in a south-east Turkey population
    (Taylor & Francis Ltd, 2017) Ay, Ozlem Izci; Balkan, Mahmut; Erdal, Mehmet Emin; Rustemoglu, Aydin; Atar, Murat; Hatipoglu, Namik Kemal; Bodakci, Mehmet Nuri
    MicroRNAs (miRNAs) are small conserved non-coding RNA molecules that post-transcriptionally regulate gene expression. Although it is reported in many studies that there are associations between alterations of miRNA homeostasis and pathological conditions such as cancer, psychiatric and neurological diseases, cardiovascular disease and autoimmune disease, the effects of common genetic variants of these genes on male infertility are unclear. To better understand this effect, we performed a case-control study including a total of 108 infertile men with idiopathic azoospermia and 125 fertile control subjects. Real-time polymerase chain reaction was used to genotype six single-nucleotide polymorphisms (SNPs) of microRNA biogenesis pathway genes and the associations between individual and combined genotypes and idiopathic azoospermia were analysed. The results showed significant difference between the individual AA genotype frequency of the GEMIN3 (rs197388) gene in the patient and control groups, indicating that the AA genotype may be considered as indicative of a higher predisposition to idiopathic azoospermia. The combined genotype analysis, including six SNPs, revealed statistically significant differences between the patients and control subjects for some combinations. For example, the frequency of genotype distributions of the AA\CA-CC-TT-AT genotype combination for the XPO5-RAN-DICER1GEMIN3 combined loci was significantly different, and it may be considered a predisposition to idiopathic azoospermia. According to the obtained results, both individual and combined genotypes of SNPs from miRNA genes may be used to predict the risk of male infertility with idiopathic azoospermia.
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    Effects of 2.4 GHz radiofrequency radiation emitted from Wi-Fi equipment on microRNA expression in brain tissue
    (Taylor & Francis Ltd, 2015) Dasdag, Suleyman; Akdag, Mehmet Zulkuf; Erdal, Mehmet Emin; Erdal, Nurten; Ay, Ozlem Izci; Ay, Mustafa Ertan; Yilmaz, Senay Gorucu
    Purpose: MicroRNAs (miRNA) play a paramount role in growth, differentiation, proliferation and cell death by suppressing one or more target genes. However, their interaction with radiofrequencies is still unknown. The aim of this study was to investigate the long-term effects of radiofrequency radiation emitted from a Wireless Fidelity (Wi-Fi) system on some of the miRNA in brain tissue. Materials and methods : The study was carried out on 16 Wistar Albino adult male rats by dividing them into two groups such as sham (n = 8) and exposure (n = 8). Rats in the exposure group were exposed to 2.4 GHz radiofrequency (RF) radiation for 24 hours a day for 12 months (one year). The same procedure was applied to the rats in the sham group except the Wi-Fi system was turned off. Immediately after the last exposure, rats were sacrificed and their brains were removed. miR-9-5p, miR-29a-3p, miR-106b-5p, miR-107, miR-125a-3p in brain were investigated in detail. Results: The results revealed that long-term exposure of 2.4 GHz Wi-Fi radiation can alter expression of some of the miRNAs such as miR-106b-5p (adj p* = 0.010) and miR-107 (adj p* = 0.005). We observed that mir 107 expression is 3.3 times and miR-106b-5p expression is 3.65 times lower in the exposure group than in the control group. However, miR-9-5p, miR-29a-3p and miR-125a-3p levels in brain were not altered. Conclusion: Long-term exposure of 2.4 GHz RF may lead to adverse effects such as neurodegenerative diseases originated from the alteration of some miRNA expression and more studies should be devoted to the effects of RF radiation on miRNA expression levels.
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    Lack of Association Between COMT Gene Polymorphism and Treatment Outcome in Major Depression
    (Yerkure Tanitim & Yayincilik Hizmetleri A S, 2011) Selek, Salih; Kaya, Mehmet C.; Erdal, Mehmet Emin; Bulut, Mahmut; Ozen, Murat Eren; Yumru, Mehmet; Barlas, Omer
    Background & Aim: Abnormal activity of Catechol-Omethyl transferase (COMT), as a major degrading enzyme of catecholaminergic neurotransmitters may be enrolled in the pathogenesis of mood disorders. The aim of this study was to investigate the association between COMT genetic polymorphism and major depression patients. Method: The study included 137 unrelated major depressive disorder (MDD) patients and 153 healthy unrelated controls, all were of Turkish origin. The patients were treated with antidepressant drugs for 8 weeks. All patients were assessed by Hamilton Depression Rating Scale (HDRS) before and after the antidepressant treatment. The analysis of COMT G1947A polymorphism was performed using PCR based endonuclease digestion method. Results: No significant difference was found between MDD and control subjects. In the MDD patients, there was no relationship between duration of illness, and pretreatment HDRS scores in respect to COMT gene polymorphism. The distribution of COMT genotypes and alleles was not significantly different among the controls and MDD patients. Conclusion: Our findings indicated that distribution of COMT genetic polymorphism were not different significantly between the patients and controls. No allele was found to be a predictor for treatment outcome by antidepressant therapy or for clinical manifestations in MDD.
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    Long term and excessive use of 900 MHz radiofrequency radiation alter microRNA expression in brain
    (Taylor & Francis Ltd, 2015) Dasdag, Suleyman; Akdag, Mehmet Zulkuf; Erdal, Mehmet Emin; Erdal, Nurten; Ay, Ozlem Izci; Ay, Mustafa Ertan; Yilmaz, Senay Gorucu
    Purpose : We still do not have any information on the interaction between radiofrequency radiation (RF) and miRNA, which play paramount role in growth, differentiation, proliferation and cell death by suppressing one or more target genes. The purpose of this study was to bridge this gap by investigating effects of long-term 900 MHz mobile phone exposure on some of the miRNA in brain tissue. Materials and methods : The study was carried out on 14 Wistar Albino adult male rats by dividing them into two groups: Sham (n = 7) and exposure (n = 7). Rats in the exposure group were exposed to 900 MHz RF radiation for 3 h per day (7 days a week) for 12 months (one year). The same procedure was applied to the rats in the sham group except the generator was turned off. Immediately after the last exposure, rats were sacrificed and their brains were removed. rno-miR-9-5p, rno-miR-29a-3p, rno-miR-106b-5p, rno-miR-107 and rno-miR-125a-3p in brain were investigated in detail. Results : Results revealed that long-term exposure of 900 MHz RF radiation only decreased rno-miR107 (adjP* = 0 .045) value where the whole body (rms) SAR value was 0.0369 W/kg. However, our results indicated that other microRNA evaluated in this study was not altered by 900 MHz RF radiation. Conclusion : 900 MHz RF radiation can alter some of the miRNA, which, in turn, may lead to adverse effects. Therefore, further studies should be performed.
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    MicroRNA dysregulation in manic and euthymic patients with bipolar disorder
    (Elsevier, 2020) Camkurt, Mehmet Akif; Karababa, Ibrahim Fatih; Erdal, Mehmet Emin; Kandemir, Sultan Basmaci; Fries, Gabriel R.; Bayazit, Huseyin; Ay, Mustafa Ertan
    Background: Bipolar disorder (BPD) is a major psychiatric disorder with an unclear pathophysiology. Peripheral blood samples are easily drawn, making them are good candidates for diagnosing diseases. MicroRNAs are small non-coding RNA transcripts that regulate gene expression by binding to the 3'-UTR of mRNAs and directing their degradation. The aim of this study was to use blood plasma to investigate microRNA dysregulations in bipolar manic and euthymic patients. Subjects and Methods: Blood samples were collected from 58 patients with bipolar I disorder (19 manic, 39 euthymic) and 51 healthy controls. Results: Four microRNAs (miR-29a-3p, p= 0.035; miR-106b-5p, p= 0.014; miR-107, p= 0.011; and miR-125a-3p, p= 0.014) were upregulated in the entire bipolar group, compared to the healthy controls. Seven microRNAs (miR-9-5p, p= 0.032; miR-29a-3p, p= 0.001; miR-106a-5p, p= 0.034; miR-106b-5p, p= 0.003; miR-107, p< 0.001; miR-125a-3p, p= 0.016; and miR-125b-5p, p= 0.004) were more upregulated in bipolar manic patients compared to the healthy controls, and two microRNAs (miR-106a-5p, p= 0.013, and miR-107, p= 0.021) showed statistically significant upregulation in the manic patients compared to the euthymic patients. Conclusions: Our results showed greater miRNA dysregulation in the manic patients than in the euthymic patients. Two microRNAs could be more selective for bipolar manic episodes. Future studies should include depressive patients along with euthymic and manic patients.
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    Possible Association of FAS and FASLG Polymorphisms with the Risk of Idiopathic Azoospermia in Southeast Turkey
    (Mary Ann Liebert, Inc, 2014) Balkan, Mahmut; Atar, Murat; Erdal, Mehmet Emin; Rustemoglu, Aydin; Yildiz, Ismail; Gunesacar, Ramazan; Hatipoglu, Namik Kemal
    To investigate the association of the genetic variants of FAS/FASLG cell death pathway genes in male infertility, we genotyped the FAS -670A/G, -1377G/A, and FASLG -124A/G single-nucleotide polymorphisms (SNPs) by real-time polymerase chain reaction in 108 infertile men with idiopathic azoospermia and in 125 proven fertile controls. The distribution of genotypes and alleles for SNPs at FAS -1377G/A and FASLG -124A/G loci were determined not to be statistically different between the case and control groups. However, the genotype frequencies of SNPs, FAS -670AA and FAS -670AG, were found to be significantly different between the case and control groups. Whereas the FAS -670AA genotype might be regarded as a higher predisposition for idiopathic azoospermia, FAS -670AG could be interpreted to mean that this genotype provides protection against idiopathic azoospermia. The study of combined genotype and haplotype frequencies has found statistically significant differences between case and control subjects for some combinations. The AA-GG binary genotype for the FAS670 and FAS1377 loci couple, in particular, may have a high degree of predisposition to idiopathic azoospermia. Our results suggest that FAS -670A/G SNP may be a genetic predisposing factor of idiopathic azoospermia among southeastern Anatolian men. Larger studies are needed to verify these findings. Furthermore, our data indicated a possible linkage between the FAS and FASLG genes and idiopathic azoospermia.
  • Küçük Resim
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    Role of 2.4 GHz radiofrequency radiation emitted from Wi-Fi on some miRNA and faty acids composition in brain
    (Taylor & Francis, 2022) Daşdağ, Süleyman; Akdağ, Mehmet Zülküf; Başhan, Mehmet; Kızmaz, Veysi; Erdal, Nurten; Erdal, Mehmet Emin
    The purpose of this study is to investigate the effects of 2.4 GHz Wi-Fi exposure, which is continuously used in the internet connection by mobile phones, computers and other wireless equipment, on microRNA and membrane and depot fatty acid composition of brain cells. Sixteen Wistar Albino rats were divided equally into two groups such as sham and exposure. The rats in the experimental group (n = 8) were exposed to 2.4 GHz RFR emitted from a Wi-Fi generator for 24 h/day for one year. The animals in the control group (n = 8) were kept under the same conditions as the experimental group, but the Wi-Fi generator was turned off. At the end of the study, rats were sacrificed and brains were removed to analyze miRNA expression and membrane and depot fatty acids of brain cells. We analyzed the situation of ten different miRNA expressions and nineteen fatty acid patterns in this study. We observed that long-term and excessive exposure of 2.4 GHz Wi-Fi radiation increased rno-miR-181a-5p, phosphatidylserine (PS) and triacylglycerol (TAG) in the brain. In conclusion, 2.4 GHz Wi-Fi exposure has the potential to alter rno-miR-181a-5p expression and the fatty acid percentage of some membrane lipids such as phospholipid (PL), phosphatidylserine (PS) and triacylglycerol (TAG), which are depot fats in the brain. However, the uncontrolled use of RFRs, whose use and diversity have reached incredible levels with each passing day and which are increasing in the future, may be paving the way for many diseases that we cannot connect with today.