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    Investigation of some radiation interaction parameters for bacteria isolated from the soil in the low energy region
    (Canadian Science Publishing, 2020) Akman, F.; Enez, B.; Fincan, S. Aguloglu; Akdemir, F.; Gecibesler, I. H.
    The purpose of this work, investigation of radiation attenuation characteristics, such as mass attenuation coefficient (mu/rho), molar extinction coefficient (epsilon), effective atomic number (Z(eff)), and electron density (N-E), of isolated bacteria from soil samples taken from Diyarbakir in Turkey. The experiments were performed using a narrow beam geometry with the help of the a high-resolution Si(Li) detector and three different radioactive sources at 15 different photon energies. The epsilon, Z(eff), and N-E values for some bacteria isolated from soil, such as Bacillus megaterium, Bacillus simplex, Bacillus subtilis, and commercially purchased Geobacillus stearothermophilus bacterium, were obtained from the measured mu/rho results in the energy range from 13.94 to 88.04 keV. The experimental values were checked with two different theoretical results and good agreement was observed among them. The results show that Bacillus simplex bacterium has the highest values of mu/rho and Z(eff) and Bacillus subtilis bacterium has the biggest values of epsilon and N-E among the present bacteria.
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    Purification and characterization of thermostable ?-amylase from thermophilic Anoxybacillus flavithermus
    (Elsevier Sci Ltd, 2014) Fincan, S. Aguloglu; Enez, B.; Ozdemir, S.; Bekler, F. Matpan
    This study reports on the purification and characterization of thermostable alpha-amylase (alpha-1-4 D-glucan glucanohydrolase EC 3.2.1.1) from a newly isolated Anoxybacillus flavithennus. A. flavithermus was used, which was isolated from hot water springs of Omer, Afyonkarahisar, Turkey. The gram-positive, spore-forming, motile, moderately thermophilic bacteria were found to be a strain of A. flavithermus analysed by 16S rRNA comparison. The optimal conditions for bacterial growth were determined to be at 20th h, 55 degrees C and pH 6.0. Maximum alpha-amylase activity was obtained at 55 degrees C at pH 7.0 after 24h of incubation. Thermostable alpha-amylase from A. flavithermus was purified by 70% (NH4)(2)SO4 and ion-exchange chromatography (5.2-fold; 65.8% yield). The molecular weight of alpha-amylase was 60 kDa, as estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The alpha-amylase hydrolyzed soluble starch at 55 degrees C with K-m: 0.005 mM and V-max: 3.5 mu mol min(-1). (C) 2013 Elsevier Ltd. All rights reserved.
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    Removal of lead (II) and toluidine blue from wastewater with new magnetic Bacillus niacini nano-biosorbent
    (Springer, 2024) Tural, B.; Ertas, E.; Enez, B.; Tural, S.
    In this study, dead Bacillus niacini microorganisms were used to support the immobilization of magnetic iron nanoparticles, creating a magnetic nano-biosorbent for wastewater treatment through magnetic separation. Magnetic nano-biosorbent was characterized via scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectrophotometry, Brunauer-Emmett-Teller analysis, and vibration sample magnetometry techniques. The laser particle sizer confirmed a uniform distribution in the particle agglomerate sizes of magnetic iron nanoparticles and magnetic nano-biosorbent, affirming successful composite formation. Energy-dispersive X-ray spectroscopy confirmed Bacillus niacini specific elements, and Fourier transform infrared spectrophotometry indicated effective Bacillus niacini coating onto magnetic iron nanoparticles. Magnetic nano-biosorbent's efficacy for toluidine blue and lead (II) removal, considering pH, contact time, magnetic nano-biosorbent dosage, and initial pollutant concentrations, was assessed. Langmuir isotherms described toluidine blue and lead (II) biosorption optimally. Kinetic data matched the pseudo-first-order and pseudo-second-order models, implying multiple biosorption mechanisms. Magnetic nano-biosorbent displayed a biosorption capacity of 66.52 +/- 0.68 mg/g for lead (II) and 82.88 +/- 0.79 mg/g for toluidine blue. Reusability tests showed effective reuse for up to five cycles. The magnetic nano-biosorbent presents significant potential for wastewater treatment due to its high biosorption capacity, efficient removal, and cost-effective synthesis.