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    Carvacrol prevents methotrexateinduced renal oxidative injury and renal damage in rats
    (2014) Bozkurt M.; Em S.; Oktayoglu P.; Turkcu G.; Yuksel H.; Sariyildiz M.A.; Caglayan M.
    Purpose: The purpose of this study was to investigate the effect of carvacrol (CAR) on methotrexate (MTX)-induced renal damage in rats. Methods: Twenty-four male rats were equally divided into three groups: group I, control treatment; group II, MTX-treated; and group III, MTX+CAR-treated. A single dose of CAR (73 mg/kg) was administered intraperitoneally to group III on the First day of the experiment and a single dose of MTX (20 mg/kg) was administered intraperitoneally to groups II and III on the second day of the experiment. Blood samples and kidney tissue were obtained from each animal on day 8 for the measurement of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI). Light microscopy was used for histopathological examination of kidney specimens. Results: MDA, TOS and OSI levels were significantly greater in the group receiving MTX alone relative to the control animals, while the TAS level was significantly reduced in the MTX group compared with the control group. The administration of CAR was associated with significantly decreased MDA, TOS, and OSI levels and increased TAS levels relative to the rats treated with MTX alone. Animals treated with CAR exhibited decreased tubular degeneration and architectural impairment relative to animals treated with MTX alone; however, the difference in histological scores did not meet the threshold of statistical significance. Conclusions: MTX treatment results in oxidative damage to the rat kidney; damage which is partially abrogated by the administration of CAR. © 2014 CIM.
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    Serum prolidase enzyme activity and oxidative status in patients with scleroderma
    (Acta Medica Mediterranea, 2014) Bozkurt M.; Dağ Ş.; Oktayoglu P.; Em S.; Yüksel H.; Çağlayan M.; Sariyildiz M.A.
    Objective: To assess serum prolidase enzyme activity and oxidative stress in patients with scleroderma (also known as systemic sclerosis, or SSc) and determine its relationship with serum oxidative status. Methods: The study population consisted of SSc patients (n = 21) and healthy participants (n =29). Serum prolidase enzyme activity, total antioxidant status (TAS), total oxidative status (TOS), oxidative stress index (OSI), and paraoxonase-1 (PON-1) levels were compared betweeen the two groups. Results: The mean duration of SSc involvement was 5 years; and the mean modified Rodnan skin score was 16. No statistically significant differences existed between the SSc groups and the control participants in terms of age, gender, TAS, PON-1, and haematocrit levels. Serum prolidase activity, OSI, and TOS levels were statistically significantly higher in SSc patients in comparison with controls participants. Also serum prolidase activity was statistically higher in Raynaud's phenomenon positive patients than Raynaud's phenomenon negative patients. But no correlation emerged between the serum prolidase activity and modified Rodnan Skin Score (P=0.235, r=0.304). Conclusion: High prolidase activity may indicate critical biological activities relevant to pathological events in SSc, and this activity may be a biological indicator of disease. Further studies are needed to verify these findings.