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Öğe The antioxidant effects of pomegranate extract on local and remote organs in a mesenteric ischemia and reperfusion model(Taylor & Francis Ltd, 2016) Kilic, Elif; Turkoglu, Ahmet; Keles, Aysenur; Ekinci, Aysun; Kesgin, Siddika; Gumus, MetehanObjectives: We investigated whether pomegranate extract plays a protective antioxidant role against mesenteric ischemia-reperfusion injury (IR), which can lead to a systemic response and damage distant organs, such as the lung, liver, and kidney. Methods: Forty female Wistar-Albino rats were separated into four groups: laparotomy, laparotomy + PG, mesenteric IR, and mesenteric IR and pomegranate (IR + PG). In the laparotomy + PG and IR + PG groups, pomegranate (225 mg/kg) was given by oral gavage at the beginning of the study. Ischemia was induced for 30 minutes, and reperfusion was subsequently allowed for 60 minutes in the IR and IR + PG groups. The malondialdehyde (MDA) and total antioxidant activity (AOA) levels were evaluated in blood samples. Additionally, all tissues were removed for the measurement of AOA and total oxidant status as well as for subsequent histopathological evaluation. The oxidative stress index was calculated. Results: Histopathological changes in all organs were significantly higher in the IR group and significantly lower in the IR + PG group vs. the other groups. Serum MDA levels were significantly lower in the IR + PG group than in the IR group. No significant difference was found in AOA levels of the groups. Discussion: These data may explain the positive protective effects of pomegranate based on the histopathologic findings in ischemic conditions in an intestinal IR injury model.Öğe Association between Serum Follistatin-Like Protein 1 Levels and Disease Activity in Behçet's Disease(Georg Thieme Verlag Kg, 2023) Demir, Mesut; Batmaz, Ibrahim; Sariyildiz, Mustafa Akif; Ekinci, Aysun; Akar, Zeynel Abidin; Batmaz, Oznur; Karakoc, MehmetIntroduction This study sought to determine the serum levels of follistatin-like protein 1 (FSTL-1), a glycoprotein of mesenchymal origin shown to be involved in the pathogenesis of many inflammatory diseases, for example Behcet's disease (BD), and to investigate its association with disease activity.Material and Methods The study included 45 patients followed for newly diagnosed Behcet's disease who met the 1990 International Study Group criteria for BD and a control group of 36 healthy volunteers who were age- and gender-matched. Peripheral blood samples were collected from the patients and control groups. Serum FSTL-1 levels were measured using an enzyme-linked immunosorbent assay. The disease activity was assessed using the Behcet's Disease Current Activity Form (BDCAF).Results A comparison of serum FSTL-1 levels in the patient and control groups found significantly higher levels of FSTL-1 in the patient group. Serum FSTL-1 levels were significantly higher in female patients compared with male patients. Female patients exhibited significantly higher BDCAF scores compared with male patients (p=0.008). Erythrocyte sedimentation rate and serum FSTL-1 levels were positively correlated.Conclusion Serum FSTL-1 levels may play a role in the aetiopathogenesis of BD and can also be a useful biomarker for determining disease activity.Öğe Comparison of fully automated urine sediment analyzers H800-FUS100 and labumat-urised with manual microscopy(John Wiley & Sons Inc, 2013) Yüksel, Hatice; Kılıç, Elif; Ekinci, Aysun; Evliyaoğlu, OsmanBackground: Recent technical developments have focused on the full automation of urinalyses, however the manual microscopic analysis of urine sediment is considered the reference method. The aim of this study was to compare the performances of the LabUMat-UriSed and the H800-FUS100 with manual microscopy, and with each other. Methods: The urine sediments of 332 urine samples were examined by these two devices (LabUMat-UriSed, H800-FUS100) and manual microscopy. Results: The reproducibility of the analyzers, UriSed and Fus100 (4.1-28.5% and 4.7-21.2%, respectively), was better than that with manual microscopy (8.5-33.3%). The UriSed was more sensitive for leukocytes (82%), while the Fus-100 was more sensitive for erythrocyte cell counting (73%). There were moderate correlations between manual microscopy and the two devices, UriSed and Fus100, for erythrocyte (r = 0.496 and 0.498, respectively) and leukocyte (r = 0.597 and 0.599, respectively) cell counting however the correlation between the two devices was much better for erythrocyte (r = 0.643) and for leukocyte (r = 0.767) cell counting. Conclusion: It can be concluded that these two devices showed similar performances. They were time-saving and standardized techniques, especially for reducing preanalytical errors such as the study time, centrifugation, and specimen volume for sedimentary analysis; however, the automated systems are still inadequate for classifying the cells that are present in pathological urine specimens. (C) 2013 Wiley Periodicals, Inc.Öğe Comparison of the effect of local microvibration and pulsed electromagnetic field application on bone fracture(Wiley-Blackwell, 2015) Bilgin, Hakki Murat; Celik, Ferhat; Akpolat, Veysi; Yildiz, Ismail; Ekinci, Aysun; Gem, Mehmet[Abstract Not Available]Öğe Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy(Taylor & Francis Ltd, 2017) Arslan, Demet; Ekinci, Aysun; Arici, Akgul; Bozdemir, Eda; Akil, Esref; Ozdemir, Hasan HuseyinDespite recent advances in antibiotic therapy, sepsis remains a major clinical challenge in intensive care units. Here we examined the anti-inflammatory and antioxidant effects of Ecballium elaterium (EE) on brain, and explored its therapeutic potential in an animal model of sepsis-associated encephalopathy (SAE) [induced by cecal ligation and puncture (CLP)]. Thirty rats were divided into three groups of 10 each: control, sepsis, and treatment. Rats were subjected to CLP except for the control group, which underwent laparatomy only. The treatment group received 2.5 mg/kg EE while the sepsis group was administered by saline. Twenty-four hours after laparotomy, animals were sacrificied and the brains were removed. Brain homogenates were prepared to assess interleukin 1beta (IL-1 beta), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha), total antioxidant capacity (TAC), and total oxidant status (TOS). Brain tissue sections were stained by hematoxylin and eosin (H&E) to semi-quantitatively examine the histopathologic changes such as neuron degeneration, pericellular/perivascular edema and inflammatory cell infiltration in the cerebral cortex. We found a statistically significant reduction in brain tissue homogenate levels of TNF-a 59.5 +/- 8.4/50.2 +/- 6.2 (p = 0.007) and TOS 99.3 +/- 16.9/82.3 +/- 7.8 (p = 0.01) in rats treated with EE; although interleukin 6 levels were increased in the treatment group compared to the sepsis group, this was not statistically significant. Neuronal damage (p = 0.00), pericellular/perivascular edema and inflammatory cell infiltration (p = 0.001) were also significantly lower in the treatment group compared to those in the sepsis group. These data suggest that Ecballium elaterium contains some components that exert protective effects against SAE in part by attenuating accumulation of proinflammatory cytokines, which may be important contributors to its anti-inflammatory effects during sepsis.Öğe Effects of local vibration and pulsed electromagnetic field on bone fracture: A comparative study(Wiley, 2017) Bilgin, Hakkı Murat; Çelik, Ferhat; Gem, Mehmet; Akpolat, Veysi; Yıldız, İsmail; Ekinci, Aysun; Özerdem, Mehmet Siraç; Tunik, Selçuk; 0000-0002-6040-9989; 0000-0003-2585-9981; 0000-0002-2435-7800; 0000-0002-0547-4139; 0000-0001-5505-838X; 0000-0002-9368-8902; 0000-0002-0549-8472The effectiveness of various therapeutic methods on bone fracture has been demonstrated in several studies. In the present study, we tried to evaluate the effect of local low-magnitude, high-frequency vibration (LMHFV) on rat tibia fracture in comparison with pulsed electromagnetic fields (PEMF) during the healing process. Mid-diaphysis tibiae fractures were induced in 30 Sprague-Dawley rats. The rats were assigned into groups such as control (CONT), LMHFV (15 min/day, 7 days/week), and PEMF (3.5 h/day, 7 days/week) for a three-week treatment. Nothing was applied to control group. Radiographs, serum osteocalcin levels, and stereological bone analyses of the three groups were compared. The X-rays of tibiae were taken 21 days after the end of the healing process. PEMF and LMHFV groups had more callus formation when compared to CONT group; however, the difference was not statistically significant (P = 0.375). Serum osteocalcin levels were elevated in the experimental groups compared to CONT (P <= 0.001). Stereological tests also showed higher osteogenic results in experimental groups, especially in LMHFV group. The results of the present study suggest that application of direct local LMHFV on fracture has promoted bone formation, showing great potential in improving fracture outcome. (C) 2017 Wiley Periodicals, Inc.Öğe Effects of Potentilla fulgens as a Prophylactic Agent for Ischemia/Reperfusion Injury in the Rat Ovary(Sci Printers & Publ Inc, 2015) Togrul, Cihan; Balsak, Deniz; Ekinci, Cenap; Seckin, Kerem Doga; Ekinci, Aysun; Tahaoglu, Ali Emre; Bademkiran, HanefiOBJECTIVE: To investigate the effects of Potentilla fulgens as a prophylactic agent on ischemia/reperfusion (I/R) injury in the rat ovary. STUDY DESIGN: A total of 32 Wistar rats were divided into 4 equal groups: (I) sham, (II) ischemia, (III) ischemia+reperfusion, and (IV) I/R+Potentilla fulgens. In groups I and II, ovary torsion was not performed and no drug was administered. In group III, 1 hour of ischemia and 2 hours of reperfusion were performed and no drug was given. Group IV received 400 mg/kg/day Potentilla fulgens intraperitoneally 5 days before I/R injury. RESULTS: The detorsion group showed preantral ovarian follicles and corpus luteum around the blood vessels and positive expression of vascular endothelial growth factor (VEGF). In the Potentilla fulgens group (IV) the stromal vascular endothelium with weak expression of VEGF was detected in small areas, and the ovarian follicles and the corpus luteum showed negative expression of VEGF. In the detorsion group the theca cells and apoptotic cells in preantral follicles showed positive expression of E-cadherin in the ovarian surface epithelium. Moreover, the E-cadherin expression was found to be positive in terms of follicular development, theca cells, granulosa cells, and corpus luteum. Potentilla fulgens, given after ischemic injury and apoptosis, was seen to decrease the effect of Bcl-2 expression. CONCLUSION: These results provide compelling evidence that the expression of E-cadherin in the ovary is an important component of ovarian function.Öğe Evaluation of New Biomarkers in the Prediction of Malignant Mesothelioma in Subjects with Environmental Asbestos Exposure(Springer, 2016) Demir, Melike; Kaya, Halide; Taylan, Mahsuk; Ekinci, Aysun; Yilmaz, Sureyya; Teke, Fatma; Sezgi, CengizhanThe purpose of this study was to investigate the potential value of certain biomarkers in predicting the presence of malignant pleural mesothelioma (MPM) in individuals environmentally exposed to asbestos. This prospective study investigated three groups; a control group composed of 41 healthy subjects, an asbestos exposure group consisting of 48 individuals, and a MPM group consisting of 42 patients. Serum levels of soluble mesothelin-related peptide (SMRP), thioredoxin-1 (TRX), epidermal growth factor receptor (EGFR), fibulin-3, syndecan-1 (SDC-1), and mesothelin were determined. Benign pleural plaques were present in 27 (58.3 %) of the individuals in the asbestos exposure group. The asbestos exposure group had significantly higher mean TRX, SMRP, and mesothelin levels compared to the control group (p = 0.023, p = 0.011, and p < 0.001, respectively). Compared to the asbestos exposure group, the MPM group had significantly higher mean EGFR, TRX, SMRP, and fibulin-3 levels (p = 0.041, p = 0.023, p = 0.002, and p = 0.001, respectively), and significantly lower mean SDC-1 levels (p = 0.002). Unlike the other biomarkers, SMRP and TRX levels increased in a graded fashion among the control, asbestos exposure, and MPM groups, respectively. Area under the curve values for SMRP and TRX were 0.86 and 0.72, respectively (95 % CI 0.79-0.92 and p < 0.001 for SMRP, and 95 % CI 0.62-0.81 and p < 0.001 for TRX). The cut-off value for SMRP was 0.62 nmol/l (sensitivity: 97.6 %, specificity: 68.9 %, positive predictive value (PPV): 56.2 %, and negative predictive value (NPV): 98.3 %) and for TRX was 156.67 ng/ml (sensitivity: 92.9 %, specificity: 77.6 %, PPV: 41.4 %, and NPV: 92.1 %). The combination of the biomarkers reached a sensitivity of 100 %, but had lower specificity (as high as 27.7 %). Serum biomarkers may be helpful for early diagnosis of MPM in asbestos-exposed cases. SMRP and TRX increased in a graded fashion from the controls to asbestos exposure and MPM groups. These two seem to be the most valuable biomarkers for the diagnosis of MPM, both individually and in combination.Öğe Expression of endothelin-1 and Ki-67 in normotensive and severe preeclamptic placentas(Universidad de la Frontera, 2018) Ekinci, Aysun; Aktaş, Ayfer; Dönder, Ahmet; Ekinci, Cenap; Turgut, Abdulkadir; Deveci, Şenay; Deveci, EnginSevere preeclampsia (HELLP syndrome) is a life-threatening pregnancy complication, usually a severe form of preeclampsia. In this study, we aimed to examine histopathologic changes and Endothelin-1 and KI-67 expression levels by immunohistochemical methods in severe preeclamptic placentas. Severe preeclampsia and obstetric characteristics and biochemical and hematological characteristics of healthy subjects were compared. Placenta sections were stained with hematoxylin-eosin for histopathological examination. In the histopathological examination of severe preeclamptic placenta, degeneration in synaptic and cytotrophoblastic cells, increase in insidious knots, fibrinoid necrosis, degeneration in endothelial cells, calcification and hyaline villous stains were observed. In the severe preeclampsia group, Ki-67 expression increased in decidua cells and inflammatory cells, while endothelial cells in the vessel wall and inflammatory cells in the villus and intervillous spaces increased. It is thought that angiogenetic and cellular proliferation is induced in a co-ordinated manner and significantly influences fetal development.Öğe Glutamine provides effective protection against deltamethrin-induced acute hepatotoxicity in rats but not against nephrotoxicity(International Scientific Literature Inc., 2015) Gündüz, Ercan; Ülger, Burak Veli; İbiloğlu, İbrahim; Ekinci, Aysun; Dursun, Recep; Zengin, Yılmaz; İçer, Mustafa; Uslukaya, Ömer; Ekinci, Cenap; Güloğlu, CahferBackground: The aim of this study was to investigate the protective effects of L-glutamine (GLN) against liver and kidney injury caused by acute toxicity of deltamethrin (DLM). Material/Methods: Thirty-two rats were indiscriminately separated into 4 groups with 8 rats each: control group (distilled water; 10 ml/kg, perorally[p.o.]), DLM group (35 mg/kg p.o. one dose.), GLN group (1.5 gr/kg, p.o. single dose.) and DLM (35 mg/kg p.o. one dose.) + GLN group (1.5 gr/kg, p.o. one dose after 4 hours.). Testing for total antioxidant status (TAS), total oxidant status (TOS), interleukin-1 beta (IL-1?), tumor necrosis factor-alpha (TNF-?), and interleukin-6 (IL-6) analyses were performed on tissue samples, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), urea, and creatinine were analyzed on serum samples. Liver and kidney samples were histopathologically analyzed. Results: The TOS level in liver was significantly higher in the DLM group than in the control group, and the level in DLM+GLN group was considerably lower than in the DLM group. The TAS level in the DLM+GLN group was considerably higher than in the control and DLM groups. The TAS level in kidney tissues was considerably lower in the DLM group than in controls, but was similar to other groups. Histopathological analyses of liver tissues established a significant difference between DLM and DLM+GLN groups in terms of grade 2 hepatic injury. However, no significant difference was found between DLM and DLM+GLN groups in terms of kidney injury. Conclusions: Glutamine leads to significant improvement in deltamethrin-induced acute hepatotoxicity in terms of histopathologic results, tissue oxidative stress parameters, and serum liver function marker enzymes.Öğe Histopathological and Biochemical Effects of Ecballium elaterium on Sepsis-Induced Lung Injury(Taylor & Francis Inc, 2016) Demir, Melike; Taylan, Mahsuk; Kaya, Halide; Ekinci, Aysun; Arslan, Demet; Aslan, Emel; Keles, AysenurPurpose: The aim of this study was to investigate the role of Ecballium elaterium (EE) on sepsis-induced lung injury. Materials and Methods: A total of 30 male rats were divided into three groups as follows: control, sepsis, and treatment groups (sepsis + EE) with each group containing 10 rats. A rat model of sepsis induced by cecal ligation and puncture (CLP) was used. In the treatment group, rats were injected intraperitoneally with 2.5mg/kg EE after CLP. Interleukin-6 (IL-6), tumor necrosis factor (TNF)-, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values after a 24-hr period were measured via cardiac puncture. Animals were harvested after the procedure and biochemical analysis was done and histopathological changes of the tissue sections of lungs were examined thereafter. Results: A statistically significant decrease was observed in the IL-6 (p < .05), TNF- (p < .05), and TOS (p < .01) levels in the sera of the treatment group compared to those of the sepsis group. Following the treatment, the TOS (p = .01) and OSI (p < .05) levels in the lung tissue of rats indicated a statistically significant decrease compared to those of the sepsis group. The histopathological follow-up undertaken after the administration of the EE treatment to septic rats showed significantly lower values of alveolar wall thickness (p < .001), interstitial edema (p = .018), and neutrophil infiltration (p = .047). Conclusion: EE treatment may have beneficial effects on sepsis-induced lung injury, and therefore has potential for clinical use.Öğe Hyperbaric Oxygen Preconditioning Provides Preliminary Protection Against Doxorubicin Cardiotoxicity(Taiwan Soc Cardiology, 2017) Tezcan, Orhan; Karahan, Oguz; Alan, Mustafa; Ekinci, Cenap; Yavuz, Celal; Demirtas, Sinan; Ekinci, AysunBackground: Doxorubicin (DOX) is generally recognized to have important cardiotoxic side effects. Studies are contradictory about the interaction between hyperbaric oxygen (HBO2) therapy and doxorubicin-induced cardiomyotoxicity. Recent data suggests that HBO2 therapy can lead to preconditioning of myocardium while generating oxidative stress. Herein we have investigated the effect of HBO2 therapy in a DOX-induced cardiomyocyte injury animal model. Methods: Twenty-one rats were divided into three equal groups as follows: 1) Group 1 is a control group (without any intervention), used for evaluating the basal cardiac structures and determining the normal value of cardiacs and serum oxidative markers; 2) Group 2 is the doxorubicin group (single dose i.p. 20 mg/kg doxorubicin) for detecting the cardiotoxic and systemic effects of doxorubicin; 3) Group 3 is the doxorubicin and HBO2 group (100% oxygen at 2.5 atmospheric for 90 minutes, daily), for evaluating the effect of HBO2 in doxorubicin induced cardiotoxicity. At the end of the protocols, the hearts were harvested and blood samples (2 ml) were obtained. Results: The doxorubicin treated animals (Group 2) had increased oxidative stress markers (both cardiac and serum) and severe cardiac injury as compared to the basal findings in the control group. Nevertheless, the highest cardiac oxidative stress index was detected in Group 3 (control vs. Group 3, p = 0.01). However, histological examination revealed that cardiac structures were well preserved in Group 3 when compared with Group 2. Conclusions: Our results suggest that HBO2 preconditioning appears to be protective in the doxorubicin-induced cardiotoxicity model. Future studies are required to better elucidate the basis of this preconditioning effect of HBO2.Öğe Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: A biochemical and histopathological study(Via Medica, 2020) Oǧuz, Abdullah; Böyük, Abdullah; Ekinci, Aysun; Alabalık, Ulaş; Türkoǧlu, Ahmet; Tuncer, Cudi M.; Ekingen, Arzu; Deveci, Engin; Gültürk, Barış; Aday, U.Background: The aim of the study was to investigate the protective effects of rosmarinic acid in rats exposed to hepatic ischaemia/reperfusion (I/R) injury. Materials and methods: Thirty-two rats were randomly classified into four groups of 8 rats each: laparotomy without medication, rosmarinic acid (dose of 50 mg/kg via oral gavage) followed by laparotomy, laparotomy followed by hepatic I/R, and hepatic I/R with rosmarinic acid. Serum aspartate aminotransferase, alanine aminotransferase, and malondialdehyde levels and total oxidant activity and total antioxidant capacity levels of the liver, lung, and kidney were assessed. The histopathologic assessment was also performed. Results: Rosmarinic acid significantly reduced liver function test parameters and decreased oxidative stress and abnormal histopathologic findings in the liver. The oxidative stress in the lung significantly increased in the I/R group but significantly decreased in the I/R + rosmarinic acid group due to the addition of rosmarinic acid. Rosmarinic acid led to no reduction in oxidative stress in kidney following hepatic I/R injury. There were no statistically significant differences among the groups regarding histopathologic changes in kidney and lung sections. Conclusions: Rosmarinic acid has antioxidant properties and is an effective hepatoprotective agent. However, although rosmarinic acid provides useful effects in the lung by increasing antioxidant capacity and reducing oxidative stress after I/R injury, it does not ameliorate histopathologic changes. These findings suggest that rosmarinic acid is likely to provide favourable outcomes in the treatment of hepatic I/R injury. CopyrightÖğe Laboratuvarımızın Preanalitik Numune Red Analizi ve Eğitimin Etkisi(2019) Ekinci, AysunAmaç: Preanalitik hataların yönetimi hasta sonuçlarınındaha etkin ve güvenilir olarak çalışılmasına katkısağlamada önemli bir yere sahiptir. Bu çalışmanın amacıpreanalitik döneme ait numune reddinin görülme sıklığınıtespit etmek, sınıflamak, eğitimin hatalar üzerine etkisinideğerlendirmektir.Gereç ve Yöntem: 2016’nın ilk altı ayı reddedilennumuneler analiz edildi. Red oranları preanalitik hatalarve analiz edildiği yere göre sınıflandırıldı. Eğitimdensonra yılın ikinci yarısında tekrar aynı analiz yapılarakeğitimin numune red oranlarına etkisi takip edildi.Bulgular: 2016 yılının ilk yarısı 641.218, son yarısı603.688 numune 1 yılda toplam 1.244.906 numune kabuledildi. İlk 6 ay 7908 son 6 ay 8150, 1 yılın sonunda16058 numune reddedildi. Toplam numune red oranı%1.29, ilk 6 ay %1.23, son 6 ay %1.35 idi. En sık nedenpıhtılı numune (%62.73) ikinci sırada yetersiz numune(%18.8) tespit edildi. Pıhtılı numuneler sıklıkla kan gazıve hemogram (tam kan sayımı) numunelerinde görüldü.Eğitim sonrasında biyokimya, hemogram ve HbA1cbiriminde çalışılan numunelerin red oranlarında azalmasaptandı (p< 0.05). Red nedenlerine göre azalma iseyanlış istem ve hatalı barkodlama olan numunelerdeistatistiksel olarak anlamlıydı (p< 0.05).Sonuç: Kaliteyi iyileştirmek için eğitimlerin sıklığınıartırmak tanımladığımız hataların tümünde beklenenazalmaya ulaşmamıza yardımcı olacaktır.Öğe The mechanism of apoptosis in human acute promyelocytic leukemia cells treated with sorafenib and lithium chloride(2019) Ekinci, Aysun; Kaya, SafiyeObjectives: This study was an investigation of the mechanisms of sorafenib (SOR) and lithium chloride (LiCl), whichcause apoptosis, in the acute promyelocytic leukemia (APL) HL-60 cell line.Methods: HL-60 cells were treated with 100 ?M of SOR, LiCl, and a combination of the 2 drugs, and a control group wasnot treated. Cells were collected after a period of 24, 48, and 72 hours, and cell proliferation and the apoptotic indexwere assessed with a hemocytometer and flow cytometry analysis. The level of caspase-3, phospho-glycogen synthasekinase-3 beta (p-GSK-3?), phospho-protein kinase B (p-AKT), phospho-extracellular-signal-regulated kinase (p-ERK),p38, phospho-c-Jun (p-c-Jun), and phospho-inhibitor kappa B (p-I?B?) were analyzed using the enzyme-linked immunosorbentassay method. The effects of the drugs on cell ultrastructure were evaluated with a transmission electronmicroscope (TEM).Results: Single and combination drug administration decreased cell proliferation and increased the apoptosis rate(p<0.01 for both). The increase in apoptosis in the SOR+LiCl group was greater than that of the SOR group (p<0.01);however, there was no significant increase compared with the LiCl group. While both drugs increased the caspase-3level (p<0.01 for both), LiCl increased caspase-3 activity more than SOR. Although p-GSK-3? levels decreased in theSOR group (p<0.01), levels increased in the LiCl group (p>0.05). Combined drug administration decreased the levelof p-AKT and p38 (p<0.01 for both); however, it did not significantly affect the level of p-ERK, p-I?B?, or p-c-Jun(p>0.05). TEM examination revealed severe lytic cytoplasmic damage and apoptotic morphology, an indication ofapoptosis.Conclusion: The results of this study demonstrated that in human APL cells treated with SOR and LiCl, increased apoptosisled to a decrease in tumor cells. This combination may become a preferred drug alternative for patients with APLand a mood disorder.Öğe Mide kanseri hastalarında survivin gen polimorfizmi araştırılması(Dicle Üniversitesi Tıp Fakültesi, 2012) Yamak, Nesibe; Yaykaşlı, Kürşat Oğuz; Soğuktaş, Hatice; Yaykaşlı, Emine; Oktay, Murat; Erdem, Havva; Kaya, Ertuğrul; Ekinci, Aysun; Kaya, Savaş; Kurman, YenerAmaç: Dünya genelinde mide kanserinin insidansı düşmesine rağmen hala önemli bir sağlık problemidir. Türkiye’de ise yılda 30.000 yeni mide kanseri vakasıyla 2. en sık görülen kanserdir. Mide kanseri genetik ve çevresel faktörlerin etkileşimiyle ortaya çıkan çok faktörlü karmaşık bir hastalıktır. Kanserli dokuda aşırı ifade edilen survivin, apoptozis inhibe edici proteinlerdendir. Bu çalışmada Survivin -31 G/C polimorfizmi ile mide kanseri arasındaki ilişki araştırıldı. Gereç ve yöntem: Çalışma Düzce Üniversitesi Araştırma ve Uygulama Hastanesi Patoloji Laboratuvarına gelen mide kanseri tanısı konmuş 46 hasta ve sağlıklı bireylerin oluşturduğu 42 kişilik kontrol grubu ile gerçekleştirildi. Bu bireylerin genotipi Düzce Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı Laboratuvarlarında PCR-RFLP yöntemiyle tayin edildi. Bulgular: Hasta grubunda, GG genotipi 16 (% 34,8), GC genotipi 21 (% 45,7) ve CC genotipi ise 9 (% 19,6) olguda saptandı. Kontrol grubunda ise, genotip dağılımı sırasıyla 13 (% 31), 26 (% 61,9) ve 3 (% 7,1) bulundu. Hasta ve kontrol grubu karşılaştırıldığında istatistiksel olarak anlamlı bir fark saptanamadı. Fakat CC genotipine sahip bireylerin mide kanserine yakalanma riskinin GG (OR=1,52) daha fazla risk oluşturduğu bulundu. Sonuç: Bu çalışma bildiğimiz kadarıyla Türk toplumunda mide kanseri ile Survivin -31G/C polimorfizmini araştıran ilk çalışmadır. Elde ettiğimiz sonuçlar hasta ve kontrol gruplarımızın temsil ettiği toplum kesitinde mide kanseri ile Survivin -31 G/C polimorfizmi arasında anlamlı bir ilişki olmadığını göstermekle birlikte CC genotipinin mide kanserine yatkınlık oluşturduğu düşünülebilir.Öğe Nebivolol Ameliorates Hepatic Ischemia/Reperfusion Injury on Liver But Not on Distant Organs(Taylor & Francis Inc, 2015) Ulger, Burak Veli; Erbis, Halil; Turkcu, Gul; Ekinci, Aysun; Turkoglu, Mehmet Akif; Ekinci, Cenap; Yilmaz, Vural TanerIntroduction: Hepatic ischemia/reperfusion injury may occur after large tumor resection and liver transplantation procedures. Nitric oxide was shown to have protective effects on ischemia/reperfusion injury. Nebivolol is a compound that has been reported to improve nitric oxide release. We evaluated the effects of nebivolol in a rat liver ischemia/reperfusion model. Methods: A total of 40 rats were randomly divided into four groups (n = 10 each). Group I underwent only laparotomy, Group II was administered nebivolol and then underwent laparotomy, Group III underwent laparotomy and hepatic ischemia/reperfusion, and Group IV was administered nebivolol and then underwent laparotomy and hepatic ischemia/reperfusion. Serum AST, ALT, urea, and creatinine levels, and TAS and TOS levels of liver, lung, and kidney tissues were determined. Histopathological determination was also performed. Results: Nebivolol significantly reduced liver function tests in group IV, but it did not improve renal functions. Oxidative stress and abnormal histopathological findings were found to be reduced in liver tissue in group IV. Although the oxidative stress was increased after hepatic ischemia/reperfusion, nebivolol could not reduce the oxidative stress in kidney tissue. There were no significant differences between group III and group IV in terms of the histopathological changes in kidney tissue. There were no significant differences in lung tissue between the groups. Conclusions: The results of this study suggest that nebivolol has protective effects on liver but not on distant organs in a hepatic ischemia/reperfusion injury model. These experimental findings indicate that nebivolol may be useful in the treatment of hepatic ischemia/reperfusion injury.Öğe Neuroprotective effects of ganoderma lucidum on spinal cord injury(Universidad de la Frontera, 2018) Ekinci, Aysun; Özevren, Hüseyin; Bilgiç, Bülent Emre; Ekinci, Cenap; Deveci, Şenay; Deveci, EnginTraumatic injury to the spinal cord results in the delayed dysfunction and neuronal death. Impaired mitochondrial function, generation of reactive oxygen species (ROS), and lipid peroxidation occur soon after traumatic spinal cord injury (SCI), while the activation of compensatory molecules that neutralize ROS occurs at later time points. The aim of the current study was to investigate the putative neuroprotective effect of Ganoderma lucidum in a rat model of SCI. In order to induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10, was used. Injured animals were given either 20 mL/kg Ganoderma lucidum or saline 30 min post injury per day by gastric gavage. At seven days postinjury, rats were decapitated. Spinal cord samples were taken for histological examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity. SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in MDA levels, MPO activity. On the other hand, Ganoderma lucidum treatment reversed all these biochemical parameters as well as SCI-induced histopathological alterations. Furthermore, impairment of the neurological functions due to SCI was improved by meloxicam treatment. The present study suggests that Ganoderma Lucidum, reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, GSH depletion.Öğe Novel markers of endothelial dysfunction and inflammation in Beh‡et's disease patients with ocular involvement: epicardial fat thickness, carotid intima media thickness, serum ADMA level, and neutrophil-to-lymphocyte ratio(Springer London Ltd, 2016) Yuksel, Murat; Yildiz, Abdulkadir; Oylumlu, Mustafa; Turkcu, Fatih Mehmet; Bilik, Mehmet Zihni; Ekinci, Aysun; Elbey, BilalThe etiology of Beh double dagger et's disease (BD) has not been fully elucidated. However, immunological and environmental factors, endothelial dysfunction (ED), and genetic susceptibility have been proposed to play a role. In this study, we aimed to evaluate epicardial fat thickness (EFT) together with serum asymmetric dimethylarginine (ADMA), carotid intima media thickness (CIMT), and neutrophil-to-lymphocyte ratio (NLR) in BD patients with ocular involvement. Thirty-six ocular BD patients (17 active and 19 inactive ocular involvement), and 35 age and sex-matched healthy controls were enrolled to this cross-sectional study. All patients underwent examinations with transthoracic echocardiography and carotid Doppler ultrasound. Serum ADMA levels, CIMT, EFT, and NLR were compared between groups, and their association with disease activity was evaluated. Beh double dagger et's disease patients had higher WBC counts, neutrophil counts, NLR, CIMT, EFT values, and serum ADMA levels than do healthy controls. The other biochemical, hematological, and echocardiographic parameters were comparable between the two groups. Beh double dagger et's disease duration was positively correlated with EFT and CIMT. Multivariate logistic regression analysis revealed that increased serum ADMA concentration and CIMT are independently associated with BD. Neutrophil counts, NLR, and serum ADMA level were higher, and lymphocyte count was lower in patients with active ocular BD compared to those of inactive ocular BD group. Carotid intima media thickness, serum ADMA level, EFT, and NLR were increased in ocular BD patients compared to healthy subjects. In addition, both serum ADMA level and NLR were associated with disease activity of ocular involvement. Increase in disease duration was associated with increase in CIMT and EFT which suggests that anatomical changes occur in time during the disease course. Increased CIMT, serum ADMA level, EFT, and NLR may provide new clues about the role of ED and inflammation in the etiopathogenesis of BD.Öğe Paraoxonase and Arylesterase Activities in Dipper and Non-Dipper Prehypertensive Subjects(Lippincott Williams & Wilkins, 2015) Yuksel, Murat; Yildiz, Abdulkadir; Tekbas, Ebru; Gunduz, Ercan; Ekinci, Aysun; Bilik, Mehmet Zihni; Ozaydogdu, NecdetParaoxonase-1, a high-density lipoprotein linked enzyme complex, was shown to be decreased in several cardiovascular diseases. We aimed to explore whether serum paraoxonase and arylesterase activities differ in dipper and non-dipper prehypertensive subjects compared to healthy controls. Sixty prehypertensive subjects and 30 controls were enrolled. All subjects underwent echocardiographic assessment and 24-hour ambulatory blood pressure monitoring (ABPM). According to the blood pressure (BP) course on ABPM, prehypertensive subjects were categorized into two: non-dipper prehypertensive (NDPH) and dipper prehypertensive (DPH) groups. Serum paraoxonase and arylesterase activities were detected spectrophotometrically. Paraoxonase and arylesterase activities were significantly lower in patients with NDPH compared to both DPH and control groups. Both paraoxonase and arylesterase activities showed significant negative correlations with BP and left ventricular mass index. We have demonstrated that NDPH subjects have lower paraoxonase and arylesterase activities compared to DPH subjects and normotensives. Further prospective studies are needed to clarify the role of paraoxonase and arylesterase activities in the development of overt hypertension in prehypertensive subjects.
