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    The effects of pulsed and sinusoidal electromagnetic fields on E-cadherin and type IV collagen in gingiva: A histopathological and immunohistochemical study
    (Wroclaw University of Medicine, 2013) Dogru A.G.; Tunik S.; Akpolat V.; Dogru M.; Saribas E.E.; Kaya F.A.; Nergiz Y.
    Background. The potential beneficial effects of extremely low frequency pulsed and sinusoidal electromagnetic fields have been shown on many tissues. Gingival epithelium plays an important role in immunosurveillance of the periodontal tissues. The epithelium acts as a mechanical barrier through cell junctions such as E-cadherin. Objectives. Investigation of the effects of extremely low frequency magnetic fields on gingiva. Material and Methods. Twenty-seven male Wistar albino rats were used. The rats were divided into three groups: control group (n = 9), SEMF group (n = 9), PEMF group (n = 9). The SEMF and PEMF (pulse time: 25 ?sn, pulse frequency: 50 Hz) groups were subjected to 1.5 mT, 50 Hz, exposure 6 h a day, 5 days a week for 28 days in methacrylate boxes. The gingival tissue pieces processed for routine histological and immunohistochemical examination and tissue sections were stained with H-E and Masson trichrome. In addition, E-cadherin and type IV collagen expressions were examined immunohistochemically. Results. Intraepithelial lymphocytes and proliferation of epithelial cells increased in both electromagnetic field groups. The over-expressions of E-cadherin on gingival epithelium was detected in the PEMF and SEMF groups. The expression level of type IV collagen was not significant between the control and electromagnetic field treated groups, except for a significant increase in the basal cell layer of the PEMF group, as compared to the control and SEMF groups. Conclusions. PEMF and SEMF have a local pro-inflammatory effect on gingiva, leading to an increase in E-cadherin level but not type IV collagen. Both PEMF and SEMF can be used as a supportive device in the treatment of gingival diseases, especially those which lead to defects in the epithelial barrier. © Copyright by Wroclaw Medical University.
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    TNF-?, IL-1? and IL-8 levels in tooth early levelling movement orthodontic treatment
    (2010) Kaya F.A.; Hamamci N.; Basaran G.; Dogru M.; Yildirim T.T.
    During orthodontic treatment, the early response of periodontal tissues to mechanical stress involves several metabolic changes that allow tooth movement. The aim of the present study was to determine tumor necrosis factor alpha (TNF-?), interleukin 1? (IL-1?) and interleukin 8 (IL-8) levels in GCF in tooth early levelling movement orthodontic treatment. Seventeen patients, 8 female and 9 male individuals (aged: 16-20 years; mean 18.2 ± 1.4 years), participated in this study. Each subject underwent a session of professional oral hygiene and received oral hygiene instructions. Three months later, a fixed orthodontic appliance was placed. The patients were seen at baseline and between 1-7 days levelling of the teeth. The concentration of TNF-?, IL-1? and IL-8 were shown in Tables 1. There were statistical differences between the observations at the 1-2 day of levelling for TNF-?, IL-1? and IL-8 (p<.05). Orthodontic forced induces rapid release of the TNF-?, 1L-1? and IL 8 levels during tooth movement in gingival crevicular fluid (GCF). The results of this study support the hypothesis that proinflammatory cytokines play a potent role in bone resorption after the application of orthodontic force in short time.
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    UHPLC-ESI-MS/MS analyses for quantification of phenolic compounds of Nepeta nuda subsp. Lydiae
    (Open Science Publishers LLP Inc., 2016) Aras A.; Bursal E.; Dogru M.
    Phenolic compounds of Nepeta nuda subsp. Lydiae leaves were identified by UHPLC-ESI-MS/MS. To the best of our knowledge, phenolic compounds in N. nuda subsp. Lydiae are reported for the first time. Chlorogenic acid, rosmarinic acid and quinic acid, were found to be the most abundant compound in the methanol extract of N. nuda subsp. Lydiae, among the twenty-seven compounds studied by LC-MS/MS. Also, smaller amounts of kaempferol, p-coumaric acid, tr-caffeic acid, apigenin, luteolin and rhamnetin were identified, quantitatively. In conclusion, leaves of N. nuda subsp. Lydiae have high potential of phenolic contents that mainly attributed with biological activities. © 2016 Abdulmelik Aras et al.