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Öğe Color stability of provisional restorative materials in different mouthwash solutions(Hindawi Limited, 2022) Değer, Yalçın; Bakır, Metin; Demirci, Fatih; Deveci, BüşraPurpose. This study is a comparison of the effects of temporary restorative materials (PRMs) on the color change that occurs due to the use of different mouthwash solutions in two time periods. Material and Methods. One hundred fifty disc-shaped specimens (10 mm × 2 mm) were fabricated with three PRMs chemically polymerized PMMA (Imident-I), chemically polymerized bisacrylic composite resin (Acrytemp-A), and CAD/CAM PMMA-based polymer (TelioCAD-T) according to manufacturers' instruction and using a CAD/CAM milling system (n = 10). CIE L∗a∗b∗ values of specimens were recorded before immersion. Samples were immersed in solutions (Distilled water-DW, Kloroben-CHX, Listerine Advanced White-LAW, Listerine Total Care-TC, and Listerine Zero-TCZ) in two time periods (t1, t2). Color measurements of the samples were made with the help of a spectrophotometer before and after they were removed in mouthwashes (VITA Easyshade V). Results. According to the statistical analysis results, significant differences were observed between the results obtained in our study. The highest roughness values in ΔE1 were seen in A-LAW (1.83) and the lowest in T-DW (0.61). In ΔE2, the highest roughness values were observed in I-LAW (2.70), and the lowest in T-DW (1.05). ΔE values of all obtained groups were found within clinically acceptable limits (ΔE < 3.7). Conclusions. The content of temporary restorative materials, the production technique, the type of mouthwash, and the immersion time of the restoration affect the color stability. Mouthwash with a whitening effect caused the most discoloration. Among the temporary restorative materials, it was the CAD/CAM material that best preserved its color stability.Öğe Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane(Via Medica, 2018) Deveci, Büşra; Ayna, Buket Erol; Tacir, İbrahim Halil; Tuncer, Cudi Mehmet; Pala, A.Background: Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of matrix metalloproteinases 2 (MMPs2) and vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane. Materials and methods: In this experimental study, rats were divided into nicotine and control groups. While the rats in the nicotine group (n = 6) were administered 2 mg/kg nicotine sulphate for 28 days, the animals in the control group (n = 6) were only administered 1.5 mL physiologic saline solution subcutaneously for 28 days. Results: Histological sections were prepared and immunohistochemically stained for MMP2 and VEGF. The sections stained with Trichrome-Masson were observed under light microscope. VEGF and MMP2 immunoreactivity of periodontal gingiva and dentin was assessed by immunohistochemical staining. Conclusions: Nicotine reduces MMP production, disrupts collagen synthesis and causes periodontitis. We observed that nicotine increases periodontitis by disrupting periodontal membrane and prevents tooth to anchor in dental alveoli by disrupting epithelial structure.Öğe The effects of nicotine on the incisive teeth and expression of vimentin in rats(2013) Faruk, E. R.; Deveci, Engin; Kalkanlı, Sevgi; Deveci, BüşraNicotine is an alkaloid toxic effects of oral cavity. In this study,14 adult Sprague-Dawley rats weighing 230-260 mg (±10 mg) were used as experimental animal. The rats of experimental group (n=6) were nicotinized systemically with nicotine sulphate, 2mg/kg subcutaneously, daily in period of 28 days. Pulp, alveolar bone tissue, periodontal membrane and gingival epithelial junction were investigated in these regions in incisive teeth longitudinal cross-section. Thinning of the collagen fibers in the pulp tissue, vascular congestion, and inflammatory cell infiltration were observed. Mesenchymal tissues that is stained positive for vimentin lay underneath the epithelium. A strong expression of vimentin can be observed in formed periodontal ligament.Öğe Sıçanların kalvaryal kemiğinde oluşturulan kemik defektlerine alloplastik kemik greft materyali kullanımı ile birlikte rosmarinik asit ve hyaluronik asit uygulamalarının histopatolojik ve immünuhistokimyasal yöntemlerle incelenmesi(Dicle Üniversitesi, Sağlık Bilimleri Enstitüsü, 2021) Deveci, Büşra; Dağ, AhmetAmaç: Greft materyalleri ile osteogenezisin stimüle edilmesini ve osteokondüktif özellikle hyaluronik asit (HA) ve rosmarinik asitin (RA) osteogenezis üzerine etkilerini ve iyileştirici özelliğini histopatolojik ve immünohistokimyasal olarak değerlendirilmesi hedeflenmiştir. Gereç ve yöntem: 48 Wistar Albino rat defekt, defekt+greft, defekt+HA, defekt+RA, defekt+greft+HA, defekt+greft+RA grupları olarak ayrıldı. Rat kalvaryumunda 8 mm çapında defekt açıldı. Alloplastik greft uygulamasından sonra HA ve RA ayrı ayrı gruplara uygulandı. 6 hafta sonunda hayvanlar sakrifiye edildi. Biyokimya, histopatoloji ve immünohistokimyasal istatistik ve değerlendirme yapıldı. Bulgular: Biyokimyasal bulgularda ALP değerleri defekt grubuna göre defekt+greft, greft+HA, greft+RA grubunda 6 hafta sonunda arttı. Kemik oluşumunu etkileyen kalsiyum, kreatin kinaz aktivitesi defekt grubunda defekt+greft, greft+HA ve greft+RA’ya göre düşük bulunmuştur. TAS değeri defekt+greft+HA ve defekt+greft+RA gruplarında defekt ve defekt+HA gruplarına göre yüksek olarak kaydedildi. OSI defekt+greft+HA ve defekt+greft+RA gruplarında defekt grubuna göre anlamlı olarak düşük bulunmuştur. Histopatolojik olarak, defekt grubunda skar doku, mononükleer hücre infiltrasyonunda artış, dejeneratif kollajen lifleri, nekrosis izlendi. Defekt + HA ve defekt+RA gruplarında yer yer küçük kemik trabekülleri ve osteoblast hücrelerinin göründüğü kemik matriks alanları gözlendi. Defekt+greft grubunda yoğun kollajen ve lif artışı, küçük kemik trabekülleri ve osteoblast hücreleri izlendi. Defekt+greft+HA ve defekt+greft+RA gruplarında greft alanı içerisindeki özellikle kemik trabeküllerinin şekillenmeye başladığı, bazı alanlarda osteoblastların belirginleştiği, osteosit lakünalarının oluştuğu gözlendi. BMP (kemik morfogenik protein)-2 ve BMP-4 ekspresyonları yoğun olarak defek+greft+HA ve defekt+greft+RA gruplarında görüldü. Sonuç: Greft sonrası HA ve RA kullanımının kemikiçi defektlerin iyileşmesinde tamamlayıcı uygulama olarak tedavi verimini arttıracağı ve yeni kemik oluşumuna katkı sağlayacağı düşüncesindeyiz.
