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Öğe Chemical Composition of The Essential Oils of Three Centaurea Species Growing Wild in Anatolia and Their Anticholinesterase Activities(Taylor & Francis Ltd, 2014) Ertas, Abdulselam; Goren, Ahmet Ceyhan; Boga, Mehmet; Demirci, Serpil; Kolak, UfukThis report represents the first study on the chemical composition of essential oil of endemic Centaurea lycopifolia. This report also represents the first study on the anticholinesterase activity of essential oils of C. lycopifolia, C. balsamita and C. iberica. Essential oils were obtained using a Clevenger apparatus by hydrodistillation from the whole parts of C. lycopifolia, C. balsamita and C. iberica. The essential oils composition of the plants were determined by GC-FID and GC-MS (gas chromatograph/mass spectrometry) analysis. The major component of the essential oils was identified as caryophyllene oxide (9.7 %) and spathulenol (7.3 %) for C. lycopifolia, alpha-selinene (8.5 %) and hexatriacontane (8.3 %) for C. balsamita and arachidic acid (25.3 %) and hexadecanoic acid (5.9 %) for C. iberica. The essential oils of three Centaurea species indicated moderate inhibitory effect against butyrylcholinesterase and acetylcholinesterase enzyme, at 200 mu g/mL.Öğe Chemical profile and biological activities of Veronica thymoides subsp pseudocinerea(Taylor & Francis Ltd, 2015) Ertas, Abdulselam; Boga, Mehmet; Kizil, Murat; Ceken, Bircan; Goren, Ahmet Ceyhan; Hasimi, Nesrin; Demirci, SerpilContext: In Turkey, Veronica species (Plantaginaceae) have been used as a diuretic and for wound healing in traditional medicine. Objective: To examine the fatty acid and essential oil profiles, the antioxidant, anticholinesterase, antimicrobial, and DNA damage effects of Veronica thymoides P.H. Davis subsp. pseudocinerea M.A. Fischer as a potential source of natural active compounds. Materials and methods: GC/MS was used to analyze essential oil and fatty acid obtained from whole plant. The antioxidant activity was evaluated by the beta-carotene-linoleic acid test system, DPPH-free and ABTS cation radicals scavenging, and cupric reducing antioxidant capacity assays. The anticholinesterase and antimicrobial activities were determined by Ellman and broth macrodillution methods, respectively. The effect of the methanol extract on DNA cleavage was investigated. Results: Hexatriacontene (21.0%) was found to be the main constituent in essential oil, and linoleic acid (25.2%) and palmitic acid (20.6%) in fatty acid. Methanol extract demonstrated the best IC50 values in lipid peroxidation (49.81 +/- 0.31 mu g/ml) and DPPH-free radical scavenging activity (15.32 +/- 0.17 mu g/ml). Methanol and water extracts possessed strong ABTS cation radical scavenging activity with IC50 values 9.15 +/- 0.28 and 8.90 +/- 0.14 mu g/ml, respectively. The acetone extract exhibited moderate butyrylcholinesterase inhibitory activity. The highest antimicrobial activity was determined in methanol extract against Escherichia coli with 31.25 mu g/ml MIC value. Inhibition of methanol extract on plasmid DNA cleavage by OH radicals was found to be 93.32% at 500 mu g/ml. Conclusion: The methanol extract having strong antioxidant and DNA damage effects could be investigated phytochemically to find natural active compounds.Öğe LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolic-flavonoid contents and biological activities(Ersin YÜCEL, 2017) Haşimi, Nesrin; Ertaş, Abdulselam; Yılmaz, Mustafa Abdullah; Boğa, Mehmet; Temel, Hamdi; Demirci, Serpil; Özden, Tuğba Yılmaz-Present work aims to determine the chemical profile and some biological activity of three endemic Astragalus species from Anatolia. The chemical contents of Astragalus leporinus var. hirsutus, Astragalus distinctissimus and Astragalus schizopterus were characterised by LC-MS/MS and GC-MS. In terms of biological activity; the antioxidant, anticholinesterase, antimicrobial and cytotoxic activities were determined. Additionally, the antioxidant properties of the major components were also determined and compared to the antioxidant capacities of these extracts. The most abundant flavonoids in these Astragalus species were determined as rutin 1028.276-13351.76 µg/g extract and hesperidin 1604.348-9695.435 µg/g extract . A high amount of quinic acid 111302.774 µg/g extract was detected in A. schizopterus methanol extract. Palmitic acid C16:0 was found to be the major compound in A. leporinus var. hirsutus 32.9% , A. distinctissimus 32.5% , and A.schizopterus 23.4% . A. schizopterus methanol extract exhibited the highest antioxidant effect in lipid peroxidation 19.62±0.29 , DPPH free 54.61±0.38 and ABTS cation radicals scavenging activity 22.01±0.07 , and CUPRAC assays. Among all of the extracts, only A. leporinus var. hirsutus petroleum ether extract showed moderate inhibitory activity against acetyl- and butyryl-cholinesterase enzymes. The methanol extracts of the plants exhibited moderate activity against C. albicans. A. leporinus var. hirsutus methanol extract indicated the most viability against L929 fibroblast cells, and the highest cytotoxic effect against A549 cells. In consideration of our findings , these Astragalus species used as animal feed could be a source of naturally, biologically active agents that can be used in food and pharmaceutical industryÖğe Phenolic Profile, Fatty Acid and Essential Oil Composition Analysis and Antioxidant, Antialzheimer and Antibacterial Activities of Verbascum flavidum Extracts(Chiang Mai Univ, 2016) Boga, Mehmet; Ertas, Abdulselam; Hasimi, Nesrin; Demirci, Serpil; Yilmaz, Mustafa Abdullah; Temel, Hamdi; Kolak, UfukThis paper is the first report about phytochemical analysis with LC-MS/MS and biological activities of Verbascum flavidum (Boiss.) Freyn & Bornm. Rutin (1077.09 +/- 77.47 mu g analyte/g extract) and chlorogenic acid (1012.42 +/- 97.18 mu g analyte/g extract) were determined as the most abundant phenolics in V. flavidum. The main components of fatty acid were found to be palmitic (30.3%) and oleic acids (17.9%); while the major essential oils were determined as arachidic acid (16.4%) and alpha-selinene (8.9%) by GC-MS analysis. In DPPH free radical scavenging activity the methanol extract showed higher activity than BHT with 72.62% inhibition at 100 mu g/mL concentration. The methanol and water extracts of V. flavidum showed 86.01 and 87.39 % inhibition in ABTS cation radical scavenging assay at 100 mu g/mL concentration, respectively. The methanol extract showed moderate activity against E. coli with 250 +/- 0.3 MIC value and with 15 mm inhibition zone diameter at 30 mg/mL concentration.
