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  • Küçük Resim
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    The effect of microwave on plasmids amplification and cell differentiation in bacillus subtilis
    (2005) Otludil, Birol; Tolan, Veysel; Otludil, Birgül Agüloğlu; Demir, R.; 0000-0002-3809-5987
    This study was carried out to investigate plasmid amplification and other differentiation by microwave irradiation (1 mV/cm2) and chloramphenicol treatment (170 ?g/ml) in Bacillus subtilis. The effect of microwave on B.subtilis was observed and the amounts of DNA, RNA, protein and plasmid were determined during the experiments. The data obtained were evaluated statistically and the variability of the amounts of DNA, Protein, RNA, Growth and plasmid exposed to microwave were less than those treated with chloramphenicol and these amounts significantly differ between microwave and chloramphenicol. Microwave exposure induces any broken fragment and the amounts of plasmids increased.
  • Küçük Resim
    Öğe
    The effects on extracellular and membrane in amylase production of the tetradentate schiff base, its mn(ii), ni(ii), cu(ii) and zn(ii) complexes and metal ions in bacillus subtilis
    (2005) Otludil, Birol; Otludil, Birgül Agüloğlu; Demir, R.; Tolan, Veysel; Temel, Hamdi; 0000-0001-9225-7425
    Bacillus subtilis was grown in mediums containing various schiff base (N, N'-Bis(salicylidene)-l,4-diaminobutane) that include transition metal complexes and metal ions. The effects of these substances on the production of secreted amylase, membrane bound amylase as well as the transport of amylase and bacterial growth were investigated. The results revelaed that the synthesis rate of amylase isolated from bacterial membranes was stimulated by growth mediums separately contains Mn(II) and Zn(II)complexes. In addition, Zn(II) complexes increased the transport amylase through the membrane, while Ni(II) and Cu(II) complexes inhibited it.