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    The relationship between Salmonella levels in chicken spleen and mechanically separated ground chicken
    (Elsevier Sci Ltd, 2016) Alali, Walid Q.; Celik, Cagatay; Wu, Diezhang; Cui, Yue; Guran, Husnu S.; Mann, David
    The objective of this study was to determine the relationship between Salmonella levels (presence and numbers) in chicken spleens and in mechanically separated chicken (MSC) at a commercial processing plant. Composite spleen and MSC samples were collected from 18 flocks at a commercial poultry processing plant. A total of 180 samples of each type were collected and tested for Salmonella using the most probable number (MPN) and enrichment methods. Overall, Salmonella was detected in 15.6% and 27.8% of spleen and MSC samples, respectively. The mean log MPN was 0.95 and 0.85 for the spleen and MSC samples, respectively. There was a significant relationship between Salmonella presence in spleen and MSC samples. However, the log MPN numbers in MSC samples were not significantly related to those in spleen samples. Salmonella presence in composite spleen samples (an indication of systemic infection in chickens) may predict MSC contamination with this pathogen, at the flock level. (C) 2016 Elsevier Ltd. All rights reserved.
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    Salmonella Levels in Turkey Neck Skins, Drumstick Bones, and Spleens in Relation to Ground Turkey
    (Int Assoc Food Protection, 2015) Cui, Yue; Guran, Husnu S.; Harrison, Mark A.; Hofacre, Charles L.; Alali, Walid Q.
    The objective of this study was to determine Salmonella levels (presence and numbers) in turkey drumstick bone, spleen, and neck skin samples in relation to Salmonella contamination levels in ground turkey at the flock level. Over a 10-month period, a total of 300 samples of each turkey part (i.e., neck skin, spleen, and drumstick) from 20 flocks were collected at a commercial turkey processing plant after the evisceration step. Turkey flocks included in this study were classified as targeted and nontargeted based on the company's historical ground turkey contamination data. A flock that originated from a turkey farm that had previously produced one or more flocks with >= 20% Salmonella prevalence in ground turkey was labeled as a targeted flock (n=13). The remaining seven flocks with <20% prevalence were labeled as nontargeted. All samples collected were tested for Salmonella presence and numbers by using most-probable-number and selective enrichment methods. Further genotypic analysis (pulsed-field gel electrophoresis) of the isolates was performed. Ground turkey samples were collected and analyzed for Salmonella levels by the cooperating turkey company. The outside surface of bone and spleen were sterilized prior to Salmonella analysis. The overall Salmonella prevalence in neck skin, drumstick bone, spleen, and ground turkey samples was 42.0, 9.3, 6.7, and 14.5%, respectively. Salmonella prevalence in neck skin, spleen, drumstick bone, and ground turkey from the targeted flocks was significantly (P < 0.05) higher than those from nontargeted flocks. There was a significant relationship between Salmonella presence in neck skin (when most probable numbers were >= 2 log) and Salmonella-positive ground turkey lot. Based on our findings, Salmonella was detected internally in drumstick bones and spleens at low levels, whereas Salmonella presence at higher levels in neck skin may indicate a flock with greater potential for Salmonella contamination of ground turkey.

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