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    DEMODEX SPECIES POSITIVITY AMONG PATIENTS WITH CANCER, ON HEMODIALYSIS AND WITH DIABETES MELLITUS
    (Southeast Asian Ministers Educ Organization, 2017) Mutlu, Fatma Yola; Cengiz, Zeynep Tas
    The objective of the present study was to determine the prevalence of Demodex sp among patients with cancer, on hemodialysis and diabetes mellitus and among controls in order to investigate if there are significance differences in Demodex sp positivity. The study was conducted at Ataturk University Research and Application Hospital, Turkey and the Yuzuncu Yil University Parasitology Laboratory, Turkey between 22 August 2011 and 31 May 2016. Study subjects consisted of 50 patients with cancer, 50 patients diagnosed with chronic renal failure and were on hemodialysis, 50 patients with diabetes mellitus and 75 healthy controls. Each patient and each control had a skin surface biopsy using a slide with cyanoacrylate applied wet to the skin until it dried and then was removed and examined under light microscopy at x10 and x40 magnification. A positive sample was one in which mites / 1 cm(2) of skin were identified. Twenty percent of the total patient group (n=150) and 5.3% of the control group (n=75) had a positive result. Among the patient groups, 26% of cancer patients, 22% of diabetes mellitus patients and 12% of patients on hemodialysis were positive. A significant association was seen between a positive skin biopsy for Demodex sp and cancer (p<0.01) and between a positive biopsy and diabetes mellitus (p<0.01). In conclusion, patients with cancer and diabetes mellitus are more likely to have a positive skin biopsy for Demodex sp than controls.
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    Human infection with Dicrocoelium dendriticum in Turkey
    (K Faisal Spec Hosp Res Centre, 2010) Cengiz, Zeynep Tas; Yilmaz, Hasan; Dulger, Ahmet Cumhur; Cicek, Mutalip
    Human dicrocoeliosis is reported sporadically in various parts of the world. We report a case in a 21-year-old male, who had right upper abdominal pain, weight loss, and chronic relapsing watery diarrhea three to four times daily for four weeks. The patient had abdominal tenderness to palpation in the right upper quadrant. Alkaline phosphatase, alanine aminotransferase, and serum immunoglobulin E levels were slightly elevated; all other biochemical and hematological findings were in their normal ranges. The duodenal biopsy samples were normal and an abdominal ultrasonography showed no biliary or hepatic abnormality. Stool microscopy revealed numerous eggs of Dicrocoelium dendriticum. As pseudoparasitosis can result from eating raw, infected animal liver, the patient was given a liver-free diet for three days, to rule out that possibility. Subsequent stool examinations showed eggs in each of the samples indicating that the infection was genuine. The patient was treated with triclabendazole 10 mg/kg in a single dose. Four weeks later, no parasite eggs were detected in the microscopic examination of the stool samples. The patient got better gradually and the symptoms disappeared. Physicians should keep in mind parasitic diseases such as the rarely encountered dicrocoeliosis.
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    Seroprevalence of human fascioliasis in Van province, Turkey
    (Aves, 2015) Cengiz, Zeynep Tas; Yilmaz, Hasan; Dulger, Ahmet Cumhur; Akdeniz, Hayrettin; Karahocagil, Mustafa Kasim; Cecek, Mutalip
    Background/Aims: Fasciola hepatica is a rare zoonotic parasite that infects the liver of many mammals including humans. The aim of this study was to determine the seroprevalence of fascioliasis in Van province by ELISA (antibody detection) on the assumption that not all cases could be detected by stool examination alone. Materials and Methods: A total of randomly selected 1,600 patients, directed from affiliated outpatient clinics to Yuzuncu Yil University Medical Faculty Parasitology Laboratory, were enrolled in the study. Their mean age was 4.44 +/- 19.00 years. Blood samples were collected from all the patients, and their stool samples were examined. For the stool examination, native-lugol and sedimentation (in formalin-ethyl acetate) methods were employed. ELISA for F. hepatica was performed on the blood samples from all patients. Seropositive patients were treated with triclabendazole. Results: F. hepatica was detected by ELISA in 89 (5.6%) of the 1,600 patients, but eggs were identified on the stool examination in only 29 (1.8%) patients. The prevalence of F. hepatica was higher in females (7.2%) than in males (4.2%) and was higher in the >= 36-year age group (6.7%) than in the <= 35-year age group (4.4%). Abdominal pain (93.3%), fatigue (88.8%), and weight loss (69.7%) were the most common symptoms. Eosinophilia was present in 89.9% of the patients. All seropositive patients had a history of eating raw aquatic plants. Conclusion: Stool examination alone is not sufficient to diagnose F. hepatica. Serological tests such as ELISA must be used together with stool examination.
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    Single-strand conformation polymorphism-based genetic characterization of the Cyclospora cayetanensis strains collected from different provinces in Turkey
    (Inst Agricultural Medicine, 2021) Cicek, Muttalip; Yildirim, Ibrahim Halil; Cengiz, Zeynep Tas; Karaman, Ulku
    Introduction and objective. Cyclospora cayetanensis, a coccidian protozoan species, has been recently found to cause diarrhea in all age groups in immunocompetent and immunocompromised individuals in most regions of the world. This study aimed to conduct the molecular detection of C. cayetanensis and to determine the genetic diversity of the 18S ribosomal RNA (rRNA) gene sequence of C. cayetanensis isolated from individuals living in different provinces in Turkey by using PCR-single-strand conformation polymorphism (SSCP). Materials and method. A total of 22 subjects were included in the study. Fourteen of the subjects were female and eight were male, with ages ranging between 7-65 years. Stool specimens were examined using wet mount and modified acid-fast staining methods, which revealed the presence of oocysts in the samples. The 18S rRNA ITS-1 Ccits37f-GCTTGCTATGTTTTAGCATGTGG and Ccits501r-GCACAATGAATGCACACACA gene regions were used as primers. The PCR products were analyzed by agarose gel electrophoresis and visualized on a UV transilluminator. For the SSCP, the PCR products were denatured with formamide, run for 16 h in 6% (49:1) polyacrylamide gel, and then imaged with silver staining. Results. SSCP assay was performed given that the DNA strands demonstrated different folds; the DNA strands contain different nucleotides based on the PCR-SSCP results for the Cyclospora strains collected in 4 provinces. Moreover, 3 different band profiles were observed in the investigated samples. A slight mutation difference was observed among the strains collected. Conclusions. Further comprehensive studies involving more C. cayetanensis-positive specimens and utilizing different mutation screening methods are warranted to demonstrate mutation differences in Cyclopora strains in Turkey.