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    LC/MS-MS analysis of phenolic compounds in Hyoscyamus albus L. extract: In vitro antidiabetic activity, in silico molecular docking, and in vivo investigation against STZ-induced diabetic mice
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023) Lekmine, Sabrina; Benslama, Ouided; Kadi, Kenza; Garcia, Antonio Ignacio Martín; Yılmaz, Mustafa Abdullah; Akkal, Salah
    This study aimed to investigate the chemical composition and antidiabetic properties of cultivated Hyoscyamus albus L. The ethanol extract was analyzed using LC-MS/MS, and 18 distinct phenolic compounds were identified. Among these, p-coumaric acid (6656.8 ± 3.4 µg/g), gallic acid (6516 ± 1.7 µg/g), luteolin (6251.9 ± 1.3 µg/g), apigenin (6209.9 ± 1.1 µg/g), and rutin (5213.9 ± 1.3 µg/g) were identified as the most abundant polyphenolic molecules. In the in vitro antidiabetic experiment, the ability of the plant extract to inhibit α-glucosidase and α-amylase activities was examined. The results indicated that the extract from H. albus L. exhibited a higher inhibitory effect on α-amylase compared to α-glucosidase, with an IC50 of 146.63 ± 1.1 µg/mL and 270.43 ± 1.1 µg/mL, respectively. Docking simulations revealed that luteolin, fisetin, and rutin exhibited the most promising inhibitory activity against both enzymes, as indicated by their high contrasting inhibition scores. To further investigate the in vivo antidiabetic effects of H. albus L., an experiment was conducted using STZ-induced diabetic mice. The results demonstrated that the plant extract effectively reduced the levels of cholesterol and triglycerides. These findings suggest that H. albus L. may have therapeutic potential for managing hyperlipidemia, a common complication associated with diabetes. This highlights its potential as a natural remedy for diabetes and related conditions.
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    Therapeutic potential of Hyoscyamus niger-derived compounds: Targeting ovarian cancer through antioxidant activity and EGFR tyrosine kinase inhibition
    (Elsevier B.V., 2024) Lekmine, Sabrina; Benslama, Ouided; Kadi, Kenza; Yılmaz, Mustafa Abdullah; Ali, Ahmad; Ola, Mohammad Shamsul; García, Antonio Ignacio Martín
    Ovarian cancer poses a significant challenge to women's health, necessitating innovative therapeutic approaches. This study investigates the therapeutic potential of compounds derived from Hyoscyamus niger, a medicinal plant with a history of traditional use, focusing on their antioxidant properties and their ability to inhibit epidermal growth factor-receptor (EGFR) tyrosine kinase a promising target in cancer therapy. LC-ESI-MS/MS analysis of H. niger extract revealed the presence of 21 phenolic compounds, with luteolin and p-coumaric acid notably exhibiting the highest concentrations. The extract displayed robust antioxidant activity across multiple assays, and its performance in the Reducing Power assay highlights its potential in reducing oxidative stress. Molecular docking results indicate that several phenolic compounds may serve as EGFR inhibitors, offering promising avenues for further investigation in cancer therapy. Notably, hyperoside emerged as a strong candidate due to its favorable binding energy and interactions within the EGFR active site. This study demonstrates the therapeutic promise of H. niger-derived compounds in targeting ovarian cancer through antioxidant activity and EGFR inhibition. Further research is warranted to validate their efficacy and safety, potentially opening up new avenues for ovarian cancer treatment.