Yazar "Basaran, Davut" seçeneğine göre listele
Listeleniyor 1 - 8 / 8
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Adventitious shoot development from leaf and stem explants of Amygdalus communis L. cv. Yaltinski(Southern Cross Publ, 2010) Isikalan, Cigdem; Akbas, Filiz; Namli, Suereyya; Basaran, DavutIn the present research effect of plant growth regulators (PGRs) on callus formation and adventitious shoot from different explants of Amygdalus communis L.cv. Yaltinski was investigated. Callus was initiated from leaf and stem explants on MS medium supplemented with four different auxin (IAA, NAA, IBA and 2, 4-D) combined with BAP (1.0 mg l(-1)) under dark and light conditions. Among all the concentrations of auxin the best result from leaf explants was observed with the 1.0: 1.0 and 2.0:1.0 NAA plus BAP treatments (90%, 88% respectively) under light conditions. Only non-embryogenic callus on leaf explants obtained in the concentrations (1.0, 2.0, 4.0 mg l(-1)) of auxin. The obtained calli from leaf explants cultured on MS media containing different concentrations of BAP (1.0, 2.0, 4.0, 6.0, 8.0 mg l(-1)) for adventitious shoot development. Calli showed some proliferation after it was transferred to the media, but did not show any embryogenic response. In our research, the highest callus induction (80%) was obtained on medium containing 1.0: 1.0 2,4-D plus BAP, under the dark (7 days) conditions for stem explants. Moreover, at the same medium and under the same conditions embryogenic calli was obtained as well. Calli showed adventitious shoot development after it was transferred to medium containing 4.0 mg l(-1) BAP, but other concentrations of BAP (2.0, 6.0, 8.0 mg l(-1)) did not show any embryogenic response. The medium supplemented with 4.0: 1.0 IAA plus BAP provided root development directly from the yaltinski stem explants under the dark conditions (7 days).Öğe Biosorption method of removal of chromium(III) accumulated with Nastunium officinale in water(Asian Journal Of Chemistry, 2008) Basaran, Davut; Opak-Kara, YesimIn present studies, Nasturtium officinale was used as a biosorption plant to remove Cr3+ present in water. For these studies, 0.1, 0.3, 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 ppm solution of Cr3+ has been prepared in water. Nasturtium officinale was immersed in the experimental solutions for 24, 48 and 72 h. The general apperance of the plants have been observed before and after 24, 48 and 72 h. The plants have been analyzed with atomic absorption for measuring the concentration. The initial and final values of concentration of Cr3+ have been found by drawing the calibration lines in UV region. The wet weight has been measured with sensitive balance. The amount of Cr3+ absorption by plant has been calculated with the dependence of the wet weight.Öğe Direct plant regeneration from in vitro-derived leaf explants of Hypericum spectabile, a medicinal plant(Academic Journals, 2011) Akbas, Filiz; Isikalan, Cigdem; Namli, Sureyya; Karakus, Pinar; Basaran, DavutAn efficient and reproducible procedure is described for direct plant regeneration using in vitro regenerated leaf explants of Hypericum spectabile. The leaf explants were cultured on MS media supplemented with six different concentrations (0.25, 0.5, 1.0, 1.5, 2.0, 2.5 mg/L) of BAP and kinetin seperately. All of the BAP concentration, shoot regeneration occurred directly without callus formation, but the number of shoots changed, depending on the different concentrations of BAP. The highest and the lowest number of shoots were obtained on the medium supplemented with 1.0 and 2.5 mg/L of BAP (90 to 50%, respectively). In the present study, the medium containing 0.25, 0.5 and 2.5 mg/L Kin did not promote adventitious shoots formation. Among concentrations of Kin, the best results in terms of both shoots number and morphogenic properties were obtained from MS medium supplemented with 1.0 mg/L Kin (60%). In vitro regenerated plants induced roots on half-strength MS medium containing all the concentrations (0.25, 0.5, 1.0, 2.0 mg/L) of IAA. However, the medium supplemented with 0.5 mg/L IAA (100%) was found to be optimum for inducing root. Rooted 5- to 6-week-old in vitro regenerated plants were transferred into a greenhouse for acclimatization.Öğe The effect of different plant hormones (PGRs) on multiple shoots of Hypericum retusum Aucher(Southern Cross Publ, 2010) Namli, Suereyya; Akbas, Filiz; Isikalan, Cigdem; Tilkat, Emine Ayaz; Basaran, DavutUse of Hypericum species have increased in the past few years due to the antidepressant and antiviral activities found in extracts of those plants. As a result of its potential as a pharmaceutical, a new system was developed for in vitro culture of this species. The goal of this investigation was to produce multiple shoot via in vitro techniques for Hypericum retusum Aucher. In vitro germination of the seeds was standardized on Murashige and Skoog (MS) hormone-free medium. Cultures were initiated from shoots inoculated onto MS medium supplemented individually with nine different concentrations of 6-Benzylaminopurine (BAP) and Kinetin (Kn). The highest number of shoots was obtained on medium supplemented with 0.5 mg l(-1) BAP (64.25 shoot/explant). Out of all the investigated concentrations of Kn, the best result was obtained on medium supplemented with 1.5 mg l(-1) Kn (27.87 shoot/explant). In addition, shoots were cultured separately on the media containing BAP (0.5 mgl(-1)) and Kn (1.5 mgl(-1)) combined with three different auxins (0.25 mgl(-1) IAA, IBA, NAA). In view of number and length of shoot the best result was obtained on the medium supplemented with 0.5 mg l(-1) BAP + 0.25 mg l(-1) IBA (54.12 shoot/explant, 3.36 length of shoot). In the presented study, the use of the BAP alone was the most efficient for shoot propagation. Rooted plantlets were acclimatized and successfully transferred to soil.Öğe Improved in vitro rooting of almond (Amygdalus communis) cultivar 'Nonpareil'(Southern Cross Publ, 2011) Namli, Sureyya; Isikalan, Cigdem; Akbas, Filiz; Basaran, DavutAn efficient method was developed for rooting of almond (Amygdalus communis L.) cultivar, Nonpariel. Apical shoots of almond (A. communis L.) Nonpareil were cultured on Murashige and Skoog (MS) medium containing 1.0 mgl(-1) BA for micropropagation. After 3 weeks cultured elongated shoots were excised and their response to a range of rooting treatments investigated. Three experiments were conducted. 1) Elongated shoots were excised and their response to a range of rooting treatments investigated. Basal end of almond shoots were dipped 1.0 g/l of IBA at different times (10, 20, 30, 40, 50 seconds) and (10,15, 20, 25, 30, 35 minutes) for rooting of almond shoots. Then, the dipped shoots were cultured on modifiye Murashige and Skoog medium (1/2 and 1/4) free hormone respectively. 2) Shoots (2-3 cm in length) were excised and the basal end dipped in 2.5, 5.0, 7.5 and 10.0 mM IBA for 3 min, then placed in the modified half strength MS medium with 2% sucrose, 0,7 % w/v agar(Agar-Agar, sigma) without plant growth regulators. Cultures were placed in the dark for 4 days prior to transfer to a 25 +/- 2 degrees C with 16 h photo period (40 mu mol m(-2) s(-1)) provided with mercury fluorescent lamps. 3) Shoots were cultured basic MS culture medium (Murashige and Skoog, 1962) containing 2.5, 5.0, 7.5 and 10.0 mu M IBA. The best root formation observed on the MS media (half strength) and dipped shoots 10, 15, 30 and 35 minutes at 1.0 g/l of IBA.Öğe In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil)(Academic Journals, 2008) Isikalan, Cigdem; Akbas, Filiz Adiyaman; Namli, Suereyya; Tilkat, Engin; Basaran, DavutAn efficient in vitro propagation method was developed for almond (Amygdalus communis L. cv. Nonpareil). The effect of BA and kinetin (0.0, 0.5, 1.0, 2.0, 4.0 mgl(-1)) on the culture initiation of zygotic embryos isolated from mature seeds was investigated. A Murashige and Skoog (1962) (MS) medium containing 30 gl(-1) sucrose, 0.5 and 1.0 mgl(-1) N-6-benzylaminopurine (BA) and 7 gl(-1) agar resulted in a multiple shoot initiation at the rate of 11.0 +/- 1.32 and 14.7 +/- 2.12 shoot per explant, respectively, in 28 days of culture. The effects of a low concentration of BA (0.1, 0.5, 1.0 and 2.0 mgl(-1)) and different combinations of auxin + cytokinin were investigated for shoot proliferation. The best results for new shoot production were obtained from a MS culture medium which was supplemented with 1.0 mgl(-1) BA. The rooting was achieved in a MS medium supplemented with 8.0 mgl(-1) indole acetic acid (IAA). The in vitro raised plants were acclimatized in a growth room and successfully transplanted to the field. This method here in described will be useful for the rapid multiplication of almond (A. communis L. cv. Nonpareil) in commercial exploitation.Öğe In vitro propagation of Diyarbakir watermelons and comparison of direct-seeded and transplanted watermelon(Tubitak Scientific & Technological Research Council Turkey, 2011) Okumus, Veysi; Pirinc, Vedat; Onay, Ahmet; Basaran, DavutA rapid protocol using shoot tip explants for micropropagation of Diyarbakir watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] types Surme, Beyazkis and Karakis was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. The effects of the different concentrations of benzyl adenine (BA) and carbohydrate types on shoot proliferation were examined. The results confirmed that a range of 0.5-1.0 mg/L of BA was almost equally effective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 genotypes studied in terms of the number of proliferated shoots and the average shoot length obtained. The highest percentage of rooting was achieved when medium supplemented with 1.0 mg/L of indole-3-butyric acid (IBA) was used for the 3 genotypes. The highest frequency of acclimatized plantlets for the 3 genotypes was 85%, 85%, and 90%, respectively, in sterile compost, when the shoots of Beyazkis, Karakis, and Surme were rooted in the IBA-supplemented rooting treatments. The Surme genotype produced a significantly higher yield and mean fruit weight than the Beyazkis and Karakis genotypes in both direct-seeded and transplanted watermelons. The main soluble sugar of the experimental types was separated, identified, and quantified using high performance liquid chromatography (H PLC). Fructose was found to be the most abundant sugar and was highly detected in Beyazkis for the seeded watermelon and in Karakis for the transplanted watermelon. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakir watermelon for use in breeding lines.Öğe Studies on Accumulation of Mn2+ by Nasturtium officinale in Water by Biosorption(Asian Journal Of Chemistry, 2009) Opak-Kara, Yesim; Basaran, Davut; Isikalan, CigdemIn present study, Nasturtium officinale has been used as a plant. The plant has been collected from the campus of Dicle university. The study has been achieved in vitro. Air roots of the plant with the same number and morphology of leaf has been chosen. For biosorption of Mn2+ metal ions by Nasturtium officinale, the solutions were prepared as 0.1; 0.3; 0.5; 1; 2; 3; 4 and 5 ppm, respectively. Water samples has been prepared from 1000 ppm stock and put them to balon joje. After this process, the plants have been transferred to the erlens and wait for 24,48 and 72 h. The erlens have been tightly closed with parafilm. The general appearence of the plants have been observed at the beginning and after 24 h. The plants has been analyzed with atomic absorption spectrometry by receiving 2 mL of water samples for calculation of concentration. The A and B values have been found by drawing the calibration lines in UV. The wet weight has been measured with sensitive balance. The quantity of Mn2+ absorption of plants has been calculated with the dependence of the wet weight.
