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    Effects of Pulsed and Sinusoidal Electromagnetic Fields on Rat Testes
    (Sci Printers & Publ Inc, 2020) Tunik, Selcuk; Baran, Ozlem Pamukcu; Akpolat, Veysi; Ayaz, Ercan; Nergiz, Yusuf; Celik, M. Salih; Yumusak, Ozkan
    OBJECTIVE: To investigate the role of 50-Hz extremely low frequency pulsed (PEMF) and sinusoidal (SEMF) electromagnetic fields on the testis using a rat model. STUDY DESIGN: Twenty-seven male Wistar albino rats were used. The rats were divided into three groups (n=9 each): sham group, SEMF group, and PEMF group. The SEMF and PEMF groups (pulse time: 20 milliseconds, pulse frequence: 50 Hz) were subjected to 1.5 mT, 50 Hz exposure 6 hours a day, 5 days a week for 28 days in methacrylate boxes. Formalin-fixed, paraffin-embedded tissue sections were stained with H-E and PAS. In addition, E-cadherin and type IV collagen expressions were examined immunohistochemically. RESULTS: Seminiferous tubule basement membranes decreased with EMFs, especially those treated with SEMF. In addition, expression levels of E-cadherin on seminiferous epithelium decreased in PEMF and SEMF groups. The expression level of type IV collagen was also decreased in perivascular and seminiferous tubule basement membrane as compared to that in the sham group. CONCLUSION: PEMF and SEMF have adverse effects on the testis at a histopathological level. EMF leads to a decrease in E-cadherin and type IV collagen expression levels.
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    Immunohistochemical Analysis of MCC, TARC and CD 104 Antigens in Human Lung Tissue
    (Ortadogu Ad Pres & Publ Co, 2010) Korkmaz, Metin; Akkus, Murat; Yildirim, Ayse; Baran, Ozlem Pamukcu; Aktas, Ayfer
    Objective: The aim of this study was to investigate the the distribution of CMA 1 protein (MCC), CCL17 protein (TARC) and Integrin beta 4 (CD 104) monoclonal antibodies in normal adult human lung tissue. Material and Methods: In this study we examined the lung biopsy specimens obtained from the adult patients who underwent operations for different reasons in the Department of Lung Surgery at Dicle University Hospital, Diyarbakir, Turkey. The tissue samples were immediately frozen in liquid nitrogen at -196 degrees C. The samples were immunostained by indirect immune peroxidase technique. As primary antibodies, MCC, TARC and CD104 were used. As secondary antibodies, 1:200 rabbit anti - mouse IgG peroxidase diluted in PBS/BSA and 1:100 normal human serum solution were used. Negative control staining was performed using irrelevant mouse monoclonal antibodies omitting the primary antibody step. Sections were examined and photographed by Olympus BH2 light microscope. Results: We observed moderate reaction with vascular endothelial cells and with vascular smooth muscle cells with MCC which was aimed to show overall the presence of human lung mast cells. TARC, which was aimed to express the follicular dentritic cells, moderately reacted with follicular dentritic cells, bronchial smooth muscle cells and vascular endothelial cells. CD104, which is the integrin beta 4 subunit that plays an important role in the adhesion of epithelium to basement membranes, moderately reacted with vascular endothelial cells and mainly reacted with the follicular dentritic cells of bronchi. Conclusion: In our study, it was defined that the expressions of these molecules have important role in understanding the function of human lung tissue. We reached the conclusion that our study, by presenting the difference between the normal lung tissue and pathologic lung tissue with expression the related proteins immunohistochemically may constitute preliminary findings for excluding the diseases like interstisial lung disease or chronic obstructive lung disease for the aim of. Our preliminary findings may shed light on future studies.
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    An increase of elastic tissue fibers in blood vessel walls of placental stem villi and differences in the thickness of blood vessel walls in third trimester pre-eclampsia pregnancies
    (Versita, 2010) Baran, Ozlem Pamukcu; Tuncer, Mehmet Cudi; Nergiz, Yusuf; Akkus, Murat; Erdemoglu, Mahmut; Buyukbayram, H.
    This study has goals of examining whether pre-eclampsia may lead to an increase of elastic tissue fibers in blood vessel walls of placental stem villi or whether there are differences in the thickness of blood vessel walls within these villi when compared to normotensive pregnant women. Non-infarcted placental tissue samples from 28 participants with uncomplicated pregnancies and 26 patients with pre-eclampsia were obtained. After routine histological procedures, the sections were processed either for conventional Verhoeff staining for the demonstration of elastic fiber system. Paraffine sections from placenta biopsies prepared for light microscopic examination were gathered. In uncomplicated pregnancies, terminal villi blood vessels were observed with no stained elastic tissue fibers in most areas. In the pre-eclampsia pregnancy of human placenta, the elastic fibers significiantly increased in terminal villi blood vessel walls which were dark in color, using Verhoeff's tissue stain, when comparing with the uncomplicated pregnancy group. Our results indicate that an increase of elastic tissue fibers in blood vessels of placental stem villus and terminal villi, and also an increase of wall thickness during pre-eclampsia.
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    THE POTENTIAL BENEFICIAL EFFECTS OF ETHYL PYRUVATE ON DIABETIC NEPHROPATHY: AN EXPERIMENTAL AND ULTRASTRUCTURAL STUDY
    (Vesalius Univ Medical Publ, 2016) Bahadir, Mehmet Veysi; Yildirim, Yasar; Baran, Ozlem Pamukcu; Polat, Sait; Akkoc, Hasan; Tunik, Selcuk
    Oxidative stress is one of the main causes of diabetic nephropathy, which is a complication of diabetes mellitus (DM). The aim of this study was to investigate the possible role of ethyl pyruvate (EP) in streptozotocin-induced diabetic rats' kidney. Four groups (n = 8) of male Wistar albino rats were used as follows: control group rats received only sodium citrate buffer solution intraperitoneally (ip). The EP group was given 50 mg/kg EP ip. In the DM group, diabetes was induced by streptozotocin. The DM + EP group received 50 mg/kg EP ip. All animals received daily treatment for 14 days, and at the end of the study the kidneys were removed: the left kidney of the rats was used for malondialdehyde (MDA) analysis and the right kidney for histological examination. There was normal appearance of the kidney tissues in the control and the EP-administered groups. In the DM group, there was evident basement membrane thickening and enlargement of mesangial matrix; swelling in some tubular epithelial cells was also noticeable. In the DM+EP administered group, nearly the same appearance as the control group and relative thickening in the glomerular basal membrane were observed. The antioxidant effect of ethyl pyruvate improved the renal structures in the DM + EP group.
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    The Prophylactic Effects of Folic Acid and Vitamin E against Valproic Acid During Fetal Thymus Development: an Ultrastructural Study
    (Soc Chilena Anatomia, 2011) Baran, Ozlem Pamukcu; Tunik, Selcuk; Akkoc, Hasan; Devieci, Engin; Ayaz, Ercan; Soker, Sevda; Tas, Sevgi Kalkanli
    To evaluate histopathologic differences in the thymus of Wistar Albino rat fetuses prenatally exposed to valproic acid (VPA), folic acid (FA) and vitamin E (Vit-E). VPA (400 mg/kg), FA (400 mcg/kg) and Vit -E (250 mg/kg) were administered to rats on each of gestation days 8,9 and 10. The fetuses (n:24) were divided into four groups: control, VPA, VPA+Vit-E and VPA+FA groups. On the 20th day of gestation, all pregnant rats were sacrificed and the fetuses were extracted. Thin sections from thymus of live fetuses were stained with uranyl acetate-lead citrate and were examined under transmission electron microscope. The histopathological findings of control group was normal. In VPA group, it showed extensive degenerative changes by VPA were on all tissue compartments when compared to controls. In VPA-FA group, vacuoles, mitochondrial cristalysis and swelling were decreased in cytoplasm. In VPA-Vit-E group, lipid storage and vacuolization were observed. Mitochondrial cristalysis decreased. Our aim in the present study is to analyze histopathological changes which may occur in a high risk experimental model after giving of VPA. In addition, protective roles of the administration of FA and Vit-E are assessed.