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    Effects of Formaldehyde and Vimentin Expression in Rat Skin
    (Soc Chilena Anatomia, 2013) Kalkanli, Sevgi; Kalkanli, Nevin; Deveci, Engin; Dal, Tuba; Baran, Ozlem
    The Wistar rats (9 weeks old, 180-200 g body weight) used in these trials were divided into two groups of 16 animals each (Control group and Experimental group). 100x65x100 in the sizes of the experimental group were taken into a glass vase. During the time period of 8 weeks, 5 days a week with 8 hours the inhalation of 10 ppm formaldehyde was made. Skin was removed and placed in 10% formaline. Sections were stained with Hematoxylene-Eosine, Trichrome-Masson and observed under light microscope. In this study, histopathological and immunohistochemical techniques due to the impact of the changes in formaldehyde and examined the distribution of vimentin.
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    Expression of Beta Human Chorionic Gonadotropin in the Placenta of Gestational Diabetic Mothers An Immunohistochemistry and Ultrastructural Study
    (Sci Printers & Publ Inc, 2013) Sak, Muhammed Erdal; Deveci, Engin; Evsen, Mehmet Siddik; Kalkanli, Sevgi; Baran, Ozlem; Ozekinci, Selver; Seker, Ugur
    OBJECTIVE: To investigate morphologic differences of the placenta in pregnancies complicated by gestational diabetes compared to nondiabetic pregnancies. STUDY DESIGN: This was a comparative morphological study of the placentas from 20 women with gestational diabetes and 20 healthy pregnancies at 28-35 weeks of gestation. RESULTS: The presence of lesions such as fibrinoid necrosis, villous edema, syncytial knot and vascular lesions like chorangiosis was apparent, mainly in the diabetes group. There was an apparent decrease in the intensity of the human chorionic gonadotropin (hCG) immunostaining in the syncytiotrophoblast from the 28th to 35th weeks of gestation in the placentas of the healthy control group. No hCG immunostaining was observed in the vinous or intervillous areas of any of the placentas. In diabetic placentas the expression of hCG was homogeneous with a moderate to intense immunoreactivity in the syncytiotrophoblast. Several syncytiotrophoblast cells showed dilations of both rough and smooth endoplasmic reticulum and loss and alteration of microvilli, and large vacuoles were observed just below the plasma membrane, as well as irregularities in the mitochondria. CONCLUSION: Syncytial cells play an important role in the placental transition. Increased expression of beta-hCG, deterioration, degeneration of organelles and cell structure and the basal membrane disorder in chorionic vessels were seen in placentas with gestational diabetes. These changes can affect placental transfer. However, further studies are needed to clarify this issue. (Anal Quant Cytopathol Histopathol 2013;35:52-56)
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    Human Placental Macrophages (Hofbauer Cells) in Severe Preeclampsia Complicated by HELLP Syndrome Immunohistochemistry of Chorionic Villi
    (Sci Printers & Publ Inc, 2013) Evsen, Mehmet Siddik; Kalkanli, Sevgi; Deveci, Engin; Sak, Muhammet Erdal; Ozler, Ali; Baran, Ozlem; Erdem, Elif
    OBJECTIVE: To evaluate Hofbauer cells in the placentas of women diagnosed with HELLP syndrome. STUDY DESIGN: The present study compared 20 patients with HELLP syndrome and 20 control patients with respect to demographics, hematological parameters and the presence of Hofbauer cells in placental samples. CD-68 antibody was used for immunohistochemical examination. The total number and size of Hofbauer cells were measured in the placental villi, and the proportion of Hofbauer cells relative to the vascular structure was also compared between groups. RESULT: The patient and control groups were similar according to baseline obstetric characteristics. White blood cell counts in patients with HELLP syndrome and the control group were 15,139 4,169 and 10,806 2,888, respectively, and were significantly increased among patients with HELLP syndrome (p < 0.001). Hofbauer cell numbers in the placental villi of patients with HELLP syndrome were significantly elevated in comparison to normotensive controls (p = 0.046). The proportion of Hofbauer cells in the placental villi according to proximity to the vascular structure were 3.85 +/- 1.66 in the HELLP group and 1.75 +/- 1.12 in controls (p < 0.001). Sizes of the Hofbauer cells were not statistically different between groups. CONCLUSION: Increased Hofbauer cells may be associated with increased inflammation or may have an adaptive mechanism at the fetal site of the placenta in patients with HELLP syndrome.