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Öğe Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium(Wiley, 2023) Aktepe, Necmettin; Baran, Ayse; Atalar, Mehmet Nuri; Baran, Mehmet Firat; Keskin, Cumali; Taskin, Abdullah; Yavuz, OemerCyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography-electrospray ionization-tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50 mg/ml, 5.0 mu g/ml). A 500 mu g/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.Öğe Determination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract(Springer Int Publ Ag, 2024) Kandemir, Sevgi İrtegun; Aktepe, Necmettin; Baran, Ayse; Baran, Mehmet Fırat; Atalar, Mehmet Nuri; Keskin, Cumali; Karadağ, MusaPurpose The phenolic composition, antioxidant, antimicrobial activity, enzyme inhibition activity, and cytotoxic activity potentials of the plant Rumex acetosella L. (R. acetosella) were examined in this study. Materials and Methods: The chemical composition of R. acetosella methanol extract was identified by the LC-MS/MS method. The antioxidant activity was tested using beta-carotene/linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC reducing power, and metal chelating activity methods. The cytotoxic activity was determined by the MTT assay using human ovarian adenocarcinoma (Skov-3), glioblastoma (U87), human dermal fibroblasts (HDF), and human colorectal adenocarcinoma (CaCo-2) cell lines. The antimicrobial activity of methanolic extracts was tested on gram-negative (Escherichia coli and Pseudomonas aeuriginosa) and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro minimum inhibition concentration method (MIC). Enzyme inhibition activity of R. acetosella methanol extract was measured spectrophotometrically against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Results: The findings showed that the major components of the methanol extract content were luteolin-7-O-glucoside (1.599 m/L), polydatin (91,024 m/L), and shikimic acid (0.773 m/L). It was determined that the extract and standard antioxidant (a-tocopherol) results in DPPH center dot, and ABTS center dot + tests performed to determine the antioxidant activity were close to each other, and this value was more effective than the standard antioxidant (alpha-tocopherol) in the CUPRAC test. These results suggested that the plant's antioxidant potential was higher when compared with reference antioxidant compounds. It was determined that the methanol extract of R. acetosella had a weaker effect on the growth of the tested microorganisms than the antibiotics used as standard. The activity of the GST and AChE enzymes was found to be severely inhibited by the methanol extract of R. acetosella. Conclusion: Based on these findings, R. acetosella L. is a medicinal and commercially beneficial plant that warrants further investigation.Öğe Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications(Taylor & Francis Inc, 2022) Atalar, Mehmet Nuri; Baran, Ayse; Baran, Mehmet Firat; Keskin, Cumali; Aktepe, Necmettin; Yavuz, Omer; Kandemir, Sevgi IrtegunIn this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.Öğe Green-synthesized nanoparticles for biomedical sensor technology(Elsevier, 2024) Baran, Ayse; Baran, Mehmet Firat; Ipek, Polat; Eftekhari, Aziz; Keskin, Cumali; Atalar, Mehmet Nuri; Alma, Mehmet HakkiSensor technology is an integral part of the many cost-effective and efficient factors possible in modern medical devices. Biosensors have good potential as they are easy, scalable, and effective in manufacturing processes. Nanotechnology has become one of the promising technologies applied in all fields of science. Biotechnologically produced metallic nanoparticles (NP) attract attention in scientific applications and technology platforms due to their extensive applications in biomedical and physiochemical fields. In recent years, the side effects caused by the use of synthetic drugs and the medical and economic problems caused by them have made the use of plants popular again. Ecofriendly, nontoxic metal-based NPs (such as gold, silver, palladium, manganese, and zinc) smaller than 100nm in size can be synthesized with extracts obtained from plants by different methods. As the sizes and shapes of NPs change, the physical, chemical, bioactive, optical, electrical, catalytic, and toxicity properties of the particles also change. Biological synthesis, also known as green synthesis, is a practical method to obtain NPs easily and ecologically without the need for high pressure, high-temperature values, and toxic chemicals. Green synthesis of NPs is carried out using different biomaterials such as bacteria, fungi, yeast, viruses, microalgae, and plant biomass/essence. Plant-mediated biosynthesis of metallic NPs occurs through biomolecules containing organic functional groups in the plant. Nanobiosensors, analytical devices combining a biologically sensitive element with a nanostructured transducer, are widely used for the molecular detection of biomarkers associated with the diagnosis of diseases and the detection of infectious organisms. Nanobiosensors show certain advantages over laboratory and many field methods due to their inherent specificity, simplicity, and rapid response. In this study, advancements in the development of nanobiosensors are illuminated. Considering all these aspects, it can be said that nanobiosensors enable diagnostic tools with increased sensitivity, specificity, and reliability for medical applications. © 2025 Elsevier Inc. All rights reserved.Öğe Potentilla fulgens root extract’s effect on breast cancer (MCF-7) and osteosarcoma (U2OS) cells proliferation and migration(2024) Ipek, Polat; Baran, AyseAim: This study evaluates the cytotoxic effect of Potentilla fulgens root extract on MCF7, U2OS, and RPE-1 cell lines and investigates its impact on cell migration. Materials and Methods: The effect of Potentilla fulgens root extract on the cell viability of MCF-7, U2OS, and RPE-1 cells was analyzed using an MTT assay. The effect of the compounds on cell migration was evaluated at 24 and 48 hours using the wound healing assay. A wound-healing assay was used to measure the metastatic ability of cells in vitro. Results: MTT assay showed that PRE had a cytotoxic effect on all three cell lines, depending on dose and time, and this effect was statistically significant. The wound healing assay results showed that PRE slowed the migration of live MCF-7 and U2OS cancer cells, and these effects increased over time. Conclusion: As a result, this study indicates that PRE may be very useful in treating human osteosarcoma and breast cancer.
