Yazar "Bacalan, Fatma" seçeneğine göre listele
Listeleniyor 1 - 5 / 5
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Culture and culture-independent diagnostic tests in Campylobacter enteritis(Journal of Infection Developing Countries, 2022) Özcan, Nida; Bacalan, Fatma; Çakır, Fatih; Bilden, Alican; Genişel, Neslihan; Dal, TubaIntroduction: Campylobacter infections are among the most common causes of bacterial enteritis. This study aims to determine the sensitivity, specificity and positive predictive values (PPV) of culture and culture-independent tests for the diagnosis of Campylobacter enteritis. Methodology: A total of 400 stool samples were included in the study. BD MAX enteric bacterial panel (BD Diagnostics, Franklin Lakes, NJ, USA) and EntericBio Gastro Panel II (Serosep, Limerick, Ireland) were used as commercial molecular tests. RIDA??QUICK Campylobacter (R-Biopharm, Darmstadt,, Germany) and CerTest (Biotec, Zaragoza, Spain) were used to detect Campylobacter antigens. Samples were cultured in CCDA media and subjected to bacterial identification by mass spectrometry. Results: Among the 400 specimens, 41 (10.2%) were evaluated as Campylobacter positive; 21 were culture-positive and 20 were detected as positive by both PCR methods. Of the 21 isolates grown in culture, 16 (76.2%) were identified as C. jejuni and 5 (23.8%) as C. coli. While all 21 culture-positive specimens were detected as positive by both molecular tests, 18 of the specimens were found positive by RidaQuick, and 16 by Certest ICA. Of the 20 culture-negative Campylobacter cases, 18 were positive by RidaQuick and 12 by Certest ICA. Sensitivities of culture, ICA-RidaQuick and ICA-CerTest were 51.2%, 87.8 and 68.3, respectively. The specificities of all tests were in the range of 90-100 %. PPV of molecular tests, ICA-RidaQuick and ICA-CerTest were > 95%, 72 % and 48.3 %, respectively. Conclusions: Molecular tests were superior to culture and ICA in terms of sensitivity, specificity, and positive predictive value.Öğe Gastroenterit etkeni olan Campylobacter türlerinin immünokromatografi, kültür-MALDI-TOF MS ve moleküler yöntemler ile saptanması(2018) Dal, Tuba; Bacalan, Fatma; Tuncel, Elif Tuğba; Özcan, NidaCampylobacter spp. bakteriyel gastroenteritlerin (GE) önde gelen ajanlarındandır. Bu çalışmada, GE vakalarından kampilobakter türlerinin kültür ile izolasyonu, immünokromatografik testler (İKT) ve moleküler yöntemlerle tanımlanması ve izolatların in vitro antibiyotik duyarlılıklarının araştırılması amaçlandı. Çalışmaya, Aralık 2016- Ocak 2018 tarihleri arasındaki 13 aylık sürede Dicle Üniversitesi Tıp Fakültesi Hastaneleri polikliniklerine ishal yakınması ile başvuran hastalara ait toplam 400 dışkı örneği dahil edildi. Örneklerde kampilobakter antijen varlığı RIDA ® QUICK Campylobacter( Biopharma, Almanya) ve CerTest (Biotec, İspanya) İKT kitleri ile, Campylobacter spp. DNA varlığı EntericBio Gastro Panel II (Serosep, İrlanda) ve BD MAX enteric bacterial panel (BD Diagnostics, ABD) ile örneklerde araştırıldı. Toplam 400 örneğin 41’i (%10.2) kültür ve/veya moleküler testlerde pozitiflik saptanarak kampilobakter GE olarak değerlendirildi. Toplam 21(%51.2) örnekte kültürde kampilobakter türleri üredi. Kültürde üreyen 21 izolatın 16 (%76.2)’sı C. jejuni, 5 (%23.8)’i C. coli olarak tanımlandı. Kampilobakter prevalansı 0-14 yaş grubunda %10.7 (24/225), 15-45 yaş aralığında %6.9 (9/131), 46 yaş üstündeki hastalarda %18.2 (8/44) olarak saptandı. İzolatlarda siprofloksasin, tetrasiklin ve eritromisin direnç oranları sırasıyla %66.7, %47.6 ve %9.5 olarak saptandı. Campylobacter spp. saptamada BD MAX EBP’nin duyarlılığı %100, özgüllüğü %96.2; EntericBio GP II’nin duyarlılığı %100, özgüllüğü %98.1 olarak bulundu. CerTest (Biotec, İspanya)’in duyarlılığı %68.3, özgüllüğü %91.6, pozitif prediktif değer (PPD) ve negatif prediktif değerleri(NPD) sırasıyla %56 ve %86 olarak saptandı. RIDA ® QUICK Campylobacter( Biopharma, Almanya) testinin duyarlılığı ve özgüllüğü sırasıyla %87.8 ve %96.1 olarak, PPD ve NPDleri %72 ve %98.6 olarak hesaplandı. Sonuç olarak tanıda kullanılan İKT’lerin kültürle doğrulanması gerekir. Yüksek özgüllük ve duyarlıkta olan moleküler sistemler ile bakteri izole edilemediği için ileri çalışma ve antibiyotik duyarlılıkları yapılamamaktadır.Öğe Microbial profile and antibiotic sensitivity pattern in bile cultures from endoscopic retrograde cholangiography patients(Baishideng Publ Grp Co Ltd, 2012) Kaya, Muhsin; Bestas, Remzi; Bacalan, Fatma; Bacaksiz, Ferhat; Arslan, Esma Gulsun; Kaplan, Mehmet AllAIM: To identify the frequency of bacterial growth, the most commonly grown bacteria and their antibiotic susceptibility, and risk factors for bacterial colonization in bile collected from patients with different biliary diseases. METHODS: This prospective study was conducted between April 2010 and August 2011. Patients with various biliary disorders were included. Bile was aspirated by placing a single-use, 5F, standard sphincterotome catheter into the bile duct before the injection of contrast agent during endoscopic retrograde cholangiopancreaticography (ERCP). Bile specimens were transported to the microbiology laboratory in blood culture bottles within an anaerobic transport system. Bacteria were cultured and identified according to the standard protocol used in our clinical microbiology laboratory. The susceptibilities of the organisms recovered were identified using antimicrobial disks, chosen according to the initial gram stain of the positive cultures. RESULTS: Ninety-one patients (27% male, mean age 53.7 +/- 17.5 years, range: 17-86 years) were included in the study. The main indication for ERCP was benign biliary disease in 79 patients and malignant disease in 12 patients. The bile culture was positive for bacterial growth in 46 out of 91 (50.5%) patients. The most frequently encountered organisms were Gram-negative bacteria including Escherichia coli (28.2%), Pseudomonas (17.3%) and Stenotrophomonas maltophilia (15.2%). There were no significant differences between patients with malignant and benign disease (58% vs 49%, P = 0.474), patients with acute cholangitis and without acute cholangitis (52.9% vs 50%, P = 0.827), patients who were empirically administered antibiotics before intervention and not administered (51.4% vs 60.7%, P = 0.384), with regard to the bacteriobilia. We observed a large covering spectrum or low resistance to meropenem, amikacin and imipenem. CONCLUSION: We did not find a significant risk factor for bacteriobilia in patients with biliary obstruction. A bile sample for microbiological analysis may become a valuable diagnostic tool as it leads to more accurate selection of antibiotics for the treatment of cholangitis. (c) 2012 Baishideng. All rights reserved.Öğe Molecular investigation of the transmission dynamics of brucellosis observed among children in the province of south - East Anatolia, Turkey(Kowsar Medical Institute, 2018) Dal, Tuba; Durmaz, Rıza; Ceylan, Ali; Bacalan, Fatma; Karagöz, Alper; Çelebi, Bekir; Yaşar, Ekrem; Kılıç, Selçuk; Açıkgöz, CibaliBackground: Brucellosis is a zoonotic disease caused by Brucella species. Although brucellosis is considered as an occupational disease in adults, recently it has become an infectious disease affecting all age groups, including children. Molecular epidemiological studies are crucial for control and treatment of disease in children. Objectives: This study aimed at identifying Brucella species, to detect antibiotic susceptibilities and define transmission dynamics between the Brucella isolates in children. Methods: A total of 77 Brucella isolates were identified by conventional and polymerase chain reaction methods. Anti - biotic susceptibilities were investigated by E - test strips. The isolates were genotyped by using multiple locus variable number tandem repeat analysis (MLVA) (MLVA - 16 Orsay), including 8 mini - satellite (panel 1) and 8 microsatellite (panel 2A and 2B) markers. Results: The mean age was 9.14±3.4 years. All patients had been consuming unpasteurized milk. All isolates were Brucella melitensis biovar 3. Only 2 isolates were resistant to ceftriaxone, while the other isolates were susceptible to other antimicrobial agents. The MLVA - 16 typing revealed 42 MLVA profiles. Eighteen profiles included 2 or more isolates, indicating a clustering rate of 66.7%. Twenty - four isolates showed a unique profile. Single locus, double locus, and 3 locus variants were detected in 32, 26, and 15 isolates, respectively. Bruce 30, Bruce 16, Bruce 9, Bruce 7, and Bruce 4 were highly discriminatory loci, respectively. All strains were defined as genotype 122, according to MLVA - 11, and genotype 43 according to MLVA - 8, and were in the Eastern Mediterranean genotype. Conclusions: High clustering rate revealed that brucellosis among the children mainly resulted from common sources. Controlling animal movements and avoiding contaminated milk products have an importance to interrupt spread of brucellosis in children.Öğe Viral and Parasitic Gastroenteritis Agents and Metronidazole Treatment in Diyarbakir Children's Hospital(Bilimsel Tip Yayinevi, 2019) Bacalan, Fatma; Cakir, Fatih; Demirkaya, Safinaz; Ozcan, NidaIntroduction: Acute gastroenteritis (AGE) agents vary according to the hygiene and sanitation conditions of the regions and age groups of the patients. Rotavirus is the most commonly reported agent in all ages, whereas adenovirus is usually detected in sporadic cases. Giardia lamblia is a common gastroenteritis protozoa worldwide. The incidence of giardiasis approaches 40% in regions with insufficient sanitation and hygiene conditions. Amibic colitis is a parasitic infection caused by Entamoeba histolytica. Metronidazole is a synthetic 5-nitroimidazole affecting anaerobic bacteria and protozoa. Unnecessary usage of metronidazole, which is widely prescribed in the treatment of amebiasis and giardiasis, may lead to antibiotic resistance as well as intestinal dysbiosis. In this study, we investigated rotavirus, adenovirus, G. lamblia and E. histolytica in the stool samples and metronidazole presciption of AGE patients admitted to our hospital. Materials and Methods: The fecal samples of 8874 patients (6 months-16 years) admitted to Diyarbakir Pediatrics Hospital with the diagnosis of AGE were retrospectively analyzed. Rotavirus, adenovirus, G. lamblia and E. histolytica/dispar agents were investigated by Enzyme Immun Assay (EIA) method using RIDASCREEN (R) (R-biopharm AG, Germany) commercial kits. Fresh stool samples were also evaluated for G. lamblia and Entamoeba cyst and trophozoites by native-lugol (NL) method. Results: Among the 8874 samples, G. lamblia (1730 samples, 19.5%) was the most common agent followed by rotavirus (1083 samples, 12.2%). Adenovirus and E. histolytica/dispar were detected in 276 (3.1%) and 189 (2.2%) samples, respectively. Among the 3162 patients who were prescribed with metronidazole, G. lamblia, rotavirus, E. histolytica/dispar and adenovirus was found in 1730, 124, 194 and 35 patients, respectively. Metronidazole was prescribed in 1238 (17.8%) of the 6950 patients who were not diagnosed as giardiasis or amoebiasis by laboratory methods. Conclusion: Metronidazole prescription rate without laboratory findings of giardiasis or amoebiasis was high in our hospital. The proper use of laboratory methods in the diagnosis of acute gastroenteritis and the regulation of treatment after laboratory-based diagnosis will reduce unnecessary antibiotics usage.
